Gene description for CD33
Gene name CD33 molecule
Gene symbol CD33
Other names/aliases SIGLEC-3
Species Homo sapiens
 Database cross references - CD33
ExoCarta ExoCarta_945
Entrez Gene 945
HGNC 1659
MIM 159590
UniProt P20138  
 CD33 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for CD33
Molecular Function
    receptor activity GO:0004872 TAS
    protein binding GO:0005515 IPI
    carbohydrate binding GO:0030246 IEA
Biological Process
    signal transduction GO:0007165 TAS
    cell-cell signaling GO:0007267 TAS
    cell adhesion GO:0007155 IEA
    negative regulation of cell proliferation GO:0008285 TAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    extracellular exosome GO:0070062 IDA
    integral component of plasma membrane GO:0005887 TAS
    external side of plasma membrane GO:0009897 IDA
 Experiment description of studies that identified CD33 in exosomes
Experiment ID 224
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for CD33
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPN6 5777
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 PTPN11 5781
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which CD33 is involved
No pathways found

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