Gene description for CBL
Gene name Cbl proto-oncogene, E3 ubiquitin protein ligase
Gene symbol CBL
Other names/aliases C-CBL
CBL2
FRA11B
NSLL
RNF55
Species Homo sapiens
 Database cross references - CBL
ExoCarta ExoCarta_867
Vesiclepedia VP_867
Entrez Gene 867
HGNC 1541
MIM 165360
UniProt P22681  
 CBL identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Neuroblastoma cells 25944692    
 Gene ontology annotations for CBL
Molecular Function
    protein binding GO:0005515 IPI
    signal transducer activity GO:0004871 IEA
    ubiquitin-protein transferase activity GO:0004842 TAS
    ephrin receptor binding GO:0046875 IPI
    sequence-specific DNA binding transcription factor activity GO:0003700 TAS
    SH3 domain binding GO:0017124 IPI
    ligase activity GO:0016874 IEA
    calcium ion binding GO:0005509 IEA
    phosphotyrosine binding GO:0001784 IEA
    zinc ion binding GO:0008270 IEA
    ubiquitin protein ligase activity GO:0061630 TAS
Biological Process
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 TAS
    negative regulation of apoptotic process GO:0043066 IMP
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    protein ubiquitination GO:0016567 TAS
    positive regulation of receptor-mediated endocytosis GO:0048260 TAS
    cell surface receptor signaling pathway GO:0007166 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    regulation of transcription, DNA-templated GO:0006355 TAS
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 IMP
    transforming growth factor beta receptor signaling pathway GO:0007179 TAS
Subcellular Localization
    flotillin complex GO:0016600 ISS
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CBL in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|CD9|FLOT1
EV Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 224
MISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for CBL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARHGEF7 8874
Two-hybrid Homo sapiens
2 SYK 6850
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 KRT18 3875
Two-hybrid Homo sapiens
4 LYN 4067
Affinity Capture-Western Homo sapiens
5 FGR 2268
Invivo Homo sapiens
6 LCK 3932
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
7 PXN  
Co-purification Homo sapiens
8 FRS2 10818
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 SPRY2 10253
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
10 GRB2 2885
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
11 SHC1 6464
Invitro Homo sapiens
12 PTPN22  
Invivo Homo sapiens
Invitro Homo sapiens
13 CSK 1445
Invivo Homo sapiens
14 SORBS1 10580
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 PTPN6 5777
Affinity Capture-Western Homo sapiens
16 Pik3r1  
Affinity Capture-Western Mus musculus
17 YWHAB 7529
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
18 INPPL1 3636
Invivo Homo sapiens
19 BLK 640
Invitro Homo sapiens
20 UBE2L3 7332
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
21 PDGFRA 5156
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
22 CD2AP 23607
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 VAV1 7409
Invivo Homo sapiens
Invitro Homo sapiens
24 TLN1 7094
Co-purification Homo sapiens
25 Grap2 17444
Reconstituted Complex Mus musculus
26 ABL1 25
Invivo Homo sapiens
Invitro Homo sapiens
27 EGFR 1956
Affinity Capture-Western Homo sapiens
28 SLA2 84174
Invivo Homo sapiens
Invitro Homo sapiens
29 HCK 3055
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 APS  
Reconstituted Complex Homo sapiens
31 MYO1C 4641
Two-hybrid Homo sapiens
32 LTK 4058
Invivo Homo sapiens
Invitro Homo sapiens
33 SH3GL3  
Affinity Capture-Western Homo sapiens
34 SH3BP2  
Reconstituted Complex Homo sapiens
35 UBE2D2 7322
Invitro Homo sapiens
36 CD5 921
Invitro Homo sapiens
37 YWHAZ 7534
Two-hybrid Homo sapiens
38 FYN 2534
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
39 FLOT1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
40 TYK2 7297
Affinity Capture-Western Homo sapiens
41 CRKL 1399
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
42 LCP2 3937
Invivo Homo sapiens
43 PTK2B 2185
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
44 ZAP70 7535
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
45 PIK3CA 5290
Co-purification Homo sapiens
46 MET 4233
Biochemical Activity Homo sapiens
47 YWHAQ 10971
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
48 PIK3R2 5296
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
49 ITK 3702
Invivo Homo sapiens
Invitro Homo sapiens
50 BTK 695
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
51 VAV2 7410
Invivo Homo sapiens
52 SLA  
Reconstituted Complex Homo sapiens
53 PLCG1 5335
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
54 ARGBP2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
55 CSF1R  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
56 SH3KBP1 30011
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
57 CD19 930
Invivo Homo sapiens
58 BCR 613
Co-purification Homo sapiens
View the network image/svg+xml



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