Gene description for FCGR2A
Gene name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Gene symbol FCGR2A
Other names/aliases CD32
CD32A
CDw32
FCG2
FCGR2
FCGR2A1
FcGR
IGFR2
Species Homo sapiens
 Database cross references - FCGR2A
ExoCarta ExoCarta_2212
Vesiclepedia VP_2212
Entrez Gene 2212
HGNC 3616
MIM 146790
UniProt P12318  
 FCGR2A identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for FCGR2A
Molecular Function
    protein binding GO:0005515 IPI
    IgG receptor activity GO:0019770 IBA
    IgG binding GO:0019864 IBA
Biological Process
    antibody-dependent cellular cytotoxicity GO:0001788 IBA
    cell surface receptor signaling pathway GO:0007166 IBA
    positive regulation of tumor necrosis factor production GO:0032760 IBA
    Fc-gamma receptor signaling pathway GO:0038094 IEA
    positive regulation of phagocytosis GO:0050766 IBA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    secretory granule membrane GO:0030667 TAS
 Experiment description of studies that identified FCGR2A in sEVs
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FCGR2A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDIPT 10423
Two-hybrid Homo sapiens
2 UPK2 7379
Two-hybrid Homo sapiens
3 STAMBP 10617
Affinity Capture-MS Homo sapiens
4 LAT 27040
Affinity Capture-Western Homo sapiens
5 PTPN11 5781
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 PLS1 5357
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PPP3R1 5534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 MIP  
Two-hybrid Homo sapiens
9 BLVRB 645
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TES 26136
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CMTM5  
Two-hybrid Homo sapiens
12 SCRN1 9805
Affinity Capture-MS Homo sapiens
13 EEA1 8411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 CRYAB 1410
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
15 NFYC 4802
Affinity Capture-MS Homo sapiens
16 LGALS3 3958
Reconstituted Complex Homo sapiens
17 PRTFDC1 56952
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 BID  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 AGFG1 3267
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 NSG1  
Two-hybrid Homo sapiens
21 ADPRHL2 54936
Affinity Capture-MS Homo sapiens
22 SERP2  
Two-hybrid Homo sapiens
23 LYN 4067
Affinity Capture-Western Homo sapiens
24 CBL 867
Co-fractionation Homo sapiens
25 APEX1 328
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 EFHD2 79180
Affinity Capture-MS Homo sapiens
27 CSGALNACT2 55454
Two-hybrid Homo sapiens
28 UBE2D2 7322
Affinity Capture-MS Homo sapiens
29 TRIM21 6737
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 ADAM33 80332
Two-hybrid Homo sapiens
31 GIMAP1  
Two-hybrid Homo sapiens
32 FCGR2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 FCGR2C 9103
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CXCL9  
Two-hybrid Homo sapiens
35 PIK3R1 5295
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
36 SYK 6850
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
37 ARPIN  
Affinity Capture-MS Homo sapiens
38 UBQLN1 29979
Two-hybrid Homo sapiens
39 FAHD2A 51011
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ITGA4 3676
Affinity Capture-MS Homo sapiens
41 PROSC 11212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 CHMP4A 29082
Affinity Capture-MS Homo sapiens
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