Gene description for FCGR2A
Gene name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Gene symbol FCGR2A
Other names/aliases CD32
CD32A
CDw32
FCG2
FCGR2
FCGR2A1
FcGR
IGFR2
Species Homo sapiens
 Database cross references - FCGR2A
ExoCarta ExoCarta_2212
Entrez Gene 2212
HGNC 3616
MIM 146790
UniProt P12318  
 FCGR2A identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for FCGR2A
Molecular Function
    protein binding GO:0005515 IPI
    IgG binding GO:0019864 IEA
Biological Process
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    innate immune response GO:0045087 TAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    extracellular exosome GO:0070062 IDA
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified FCGR2A in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FCGR2A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LYN 4067
Affinity Capture-Western Homo sapiens
2 LGALS3 3958
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 PIK3R1 5295
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 LAT 27040
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
5 SYK 6850
Reconstituted Complex Homo sapiens
6 SHC1 6464
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
7 HCK 3055
Affinity Capture-MS Homo sapiens
8 CRP  
Invivo Homo sapiens
View the network image/svg+xml



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