Gene description for SYK
Gene name spleen tyrosine kinase
Gene symbol SYK
Other names/aliases p72-Syk
Species Homo sapiens
 Database cross references - SYK
ExoCarta ExoCarta_6850
Vesiclepedia VP_6850
Entrez Gene 6850
HGNC 11491
MIM 600085
UniProt P43405  
 SYK identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Hepatocytes 26054723    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for SYK
Molecular Function
    ATP binding GO:0005524 IEA
    integrin binding GO:0005178 IPI
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IEA
    protein tyrosine kinase activity GO:0004713 TAS
    protein kinase activity GO:0004672 NAS
    non-membrane spanning protein tyrosine kinase activity GO:0004715 TAS
    receptor signaling protein tyrosine kinase activity GO:0004716 IEA
    protein serine/threonine kinase activity GO:0004674 IDA
Biological Process
    peptidyl-tyrosine autophosphorylation GO:0038083 IBA
    regulation of tumor necrosis factor-mediated signaling pathway GO:0010803 IMP
    activation of JUN kinase activity GO:0007257 IEA
    viral process GO:0016032 IEA
    neutrophil activation involved in immune response GO:0002283 ISS
    positive regulation of alpha-beta T cell proliferation GO:0046641 IBA
    lymph vessel development GO:0001945 ISS
    positive regulation of interleukin-3 biosynthetic process GO:0045401 IEA
    neutrophil chemotaxis GO:0030593 IDA
    organ morphogenesis GO:0009887 TAS
    transcription factor import into nucleus GO:0042991 IMP
    leukocyte activation involved in immune response GO:0002366 ISS
    defense response to bacterium GO:0042742 ISS
    protein phosphorylation GO:0006468 ISS
    blood vessel morphogenesis GO:0048514 ISS
    regulation of platelet aggregation GO:0090330 ISS
    regulation of ERK1 and ERK2 cascade GO:0070372 ISS
    positive regulation of gamma-delta T cell differentiation GO:0045588 IEA
    positive regulation of B cell differentiation GO:0045579 IMP
    blood coagulation GO:0007596 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    positive regulation of mast cell degranulation GO:0043306 IBA
    positive regulation of calcium-mediated signaling GO:0050850 IEA
    macrophage activation involved in immune response GO:0002281 ISS
    positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process GO:0045425 IEA
    beta selection GO:0043366 IEA
    leukocyte cell-cell adhesion GO:0007159 IDA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    regulation of superoxide anion generation GO:0032928 ISS
    regulation of platelet activation GO:0010543 ISS
    serotonin secretion by platelet GO:0002554 ISS
    leukotriene biosynthetic process GO:0019370 IEA
    adaptive immune response GO:0002250 ISS
    B cell receptor signaling pathway GO:0050853 ISS
    regulation of sequence-specific DNA binding transcription factor activity GO:0051090 IMP
    cellular response to molecule of fungal origin GO:0071226 ISS
    innate immune response GO:0045087 TAS
    positive regulation of cytokine secretion GO:0050715 IEA
    positive regulation of cell adhesion mediated by integrin GO:0033630 ISS
    positive regulation of alpha-beta T cell differentiation GO:0046638 IEA
    regulation of arachidonic acid secretion GO:0090237 ISS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    angiogenesis GO:0001525 IEA
    cell proliferation GO:0008283 TAS
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    platelet activation GO:0030168 TAS
    positive regulation of bone resorption GO:0045780 ISS
    integrin-mediated signaling pathway GO:0007229 NAS
    regulation of phagocytosis GO:0050764 ISS
    regulation of neutrophil degranulation GO:0043313 ISS
    cell differentiation GO:0030154 IBA
Subcellular Localization
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IBA
    early phagosome GO:0032009 ISS
    cytosol GO:0005829 TAS
    T cell receptor complex GO:0042101 IDA
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 TAS
    B cell receptor complex GO:0019815 IEA
    nucleus GO:0005634 IDA
    protein complex GO:0043234 IDA
 Experiment description of studies that identified SYK in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SYK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BLNK  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
2 LYN 4067
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 FCGR1A  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
4 VAV1 7409
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
5 LAT 27040
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
6 CBLB 868
Affinity Capture-MS Homo sapiens
7 CSF3R  
Invitro Homo sapiens
8 FGR 2268
Invivo Homo sapiens
Invitro Homo sapiens
9 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
10 PXN  
Invivo Homo sapiens
Invitro Homo sapiens
11 SLA  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 CD22 933
Invitro Homo sapiens
13 PTPN6 5777
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
14 STAT5A 6776
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
15 CD79B 974
Reconstituted Complex Homo sapiens
16 CD72  
Biochemical Activity Homo sapiens
17 PAG1 55824
Reconstituted Complex Homo sapiens
18 EZR 7430
Affinity Capture-MS Homo sapiens
19 FCER1G 2207
Reconstituted Complex Homo sapiens
20 LCK 3932
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
21 TRAF6 7189
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
22 CTTN 2017
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
23 PRKD1 5587
Affinity Capture-Western Homo sapiens
24 FCGR2A 2212
Reconstituted Complex Homo sapiens
25 MS4A2  
Reconstituted Complex Homo sapiens
26 CD79A  
Reconstituted Complex Homo sapiens
27 RPS6KB2  
Biochemical Activity Homo sapiens
28 TUBA1A 7846
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
29 CBL 867
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
30 UBB 7314
Affinity Capture-MS Homo sapiens
31 RPS6KB1 6198
Biochemical Activity Homo sapiens
32 PTK2 5747
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
33 FYN 2534
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
34 JAK1 3716
Invitro Homo sapiens
35 CRKL 1399
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
36 LCP2 3937
Affinity Capture-MS Homo sapiens
37 PTK2B 2185
Affinity Capture-Western Homo sapiens
38 SIT1 27240
Invivo Homo sapiens
39 SRC 6714
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
40 STAT3 6774
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
41 SYK 6850
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
42 SH3BP2  
Two-hybrid Homo sapiens
43 CD3E 916
Invivo Homo sapiens
Invitro Homo sapiens
44 ITGB2 3689
Affinity Capture-Western Homo sapiens
45 PLCG1 5335
Affinity Capture-Western Homo sapiens
46 CD19 930
Invivo Homo sapiens
47 EPOR  
Invivo Homo sapiens
Invitro Homo sapiens
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