Gene description for PAG1
Gene name phosphoprotein membrane anchor with glycosphingolipid microdomains 1
Gene symbol PAG1
Other names/aliases CBP
PAG
Species Homo sapiens
 Database cross references - PAG1
ExoCarta ExoCarta_55824
Vesiclepedia VP_55824
Entrez Gene 55824
HGNC 30043
MIM 605767
UniProt Q9NWQ8  
 PAG1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PAG1
Molecular Function
    protein binding GO:0005515 IPI
    signaling adaptor activity GO:0035591 NAS
    SH2 domain binding GO:0042169 IDA
Biological Process
    adaptive immune response GO:0002250 IEA
    signal transduction GO:0007165 TAS
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 IDA
    regulation of T cell activation GO:0050863 IDA
    negative regulation of T cell activation GO:0050868 IBA
Subcellular Localization
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    membrane raft GO:0045121 IBA
    membrane raft GO:0045121 IDA
 Experiment description of studies that identified PAG1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PAG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DUSP22  
Affinity Capture-MS Homo sapiens
2 TAS2R19  
Affinity Capture-MS Homo sapiens
3 SERHL2  
Affinity Capture-MS Homo sapiens
4 LCK 3932
Reconstituted Complex Homo sapiens
5 RAB35 11021
Proximity Label-MS Homo sapiens
6 GPR141  
Affinity Capture-MS Homo sapiens
7 NCSTN 23385
Affinity Capture-MS Homo sapiens
8 GRB2 2885
Reconstituted Complex Homo sapiens
9 SHC1 6464
Reconstituted Complex Homo sapiens
10 PMS1  
Affinity Capture-MS Homo sapiens
11 CSK 1445
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 MARCKS 4082
Proximity Label-MS Homo sapiens
13 PTPN6 5777
Reconstituted Complex Homo sapiens
14 S1PR2 9294
Affinity Capture-MS Homo sapiens
15 HOXB5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ZNF517  
Affinity Capture-MS Homo sapiens
17 GFAP 2670
Affinity Capture-MS Homo sapiens
18 RABIF  
Affinity Capture-MS Homo sapiens
19 GSTK1 373156
Affinity Capture-MS Homo sapiens
20 CYP2S1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 VAV1 7409
Reconstituted Complex Homo sapiens
22 EFNB2 1948
Affinity Capture-Western Homo sapiens
23 PCK2 5106
Affinity Capture-MS Homo sapiens
24 ABL1 25
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
25 PCDHGC3 5098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 AGO2 27161
Affinity Capture-RNA Homo sapiens
27 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 LYN 4067
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
29 MCAM 4162
Proximity Label-MS Homo sapiens
30 SRC 6714
Affinity Capture-Western Homo sapiens
31 RPS27 6232
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 GJB7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 MED21  
Affinity Capture-MS Homo sapiens
34 PAG1 55824
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 NTRK3 4916
Affinity Capture-MS Homo sapiens
36 CDH1 999
Proximity Label-MS Homo sapiens
37 FYN 2534
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
38 TIGD5  
Affinity Capture-MS Homo sapiens
39 ACPP 55
Affinity Capture-MS Homo sapiens
40 LCP2 3937
Reconstituted Complex Homo sapiens
41 SLC9A3R1 9368
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
42 ZAP70 7535
Reconstituted Complex Homo sapiens
43 EEF1A2 1917
Affinity Capture-MS Homo sapiens
44 EPHA2 1969
Proximity Label-MS Homo sapiens
45 DIXDC1  
Affinity Capture-MS Homo sapiens
46 ACBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 KRAS 3845
Proximity Label-MS Homo sapiens
48 CLPB 81570
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 SYK 6850
Reconstituted Complex Homo sapiens
50 MTUS2 23281
Affinity Capture-MS Homo sapiens
51 CCR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 CAV1 857
Proximity Label-MS Homo sapiens
53 RHOB 388
Proximity Label-MS Homo sapiens
54 FKBP11 51303
Affinity Capture-MS Homo sapiens
55 C1QL4  
Affinity Capture-MS Homo sapiens
56 CERS2 29956
Affinity Capture-MS Homo sapiens
57 TMEM185A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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