Gene description for LCP2
Gene name lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
Gene symbol LCP2
Other names/aliases SLP-76
SLP76
Species Homo sapiens
 Database cross references - LCP2
ExoCarta ExoCarta_3937
Entrez Gene 3937
HGNC 6529
MIM 601603
UniProt Q13094  
 LCP2 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for LCP2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 TAS
    T cell receptor signaling pathway GO:0050852 TAS
    mast cell activation GO:0045576 IEA
    intracellular signal transduction GO:0035556 IBA
    innate immune response GO:0045087 TAS
    platelet activation GO:0030168 TAS
    positive regulation of protein kinase activity GO:0045860 IEP
    immune response GO:0006955 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    blood coagulation GO:0007596 TAS
    cytokine secretion GO:0050663 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    cell-cell junction GO:0005911 IEA
 Experiment description of studies that identified LCP2 in exosomes
1
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LCP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PLCG2 5336
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
2 FYB 2533
Invitro Homo sapiens
3 GRAP2 9402
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 TRAT1 50852
Reconstituted Complex Homo sapiens
5 LYN 4067
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 ITK 3702
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 LAT 27040
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
8 SYK 6850
Affinity Capture-MS Homo sapiens
9 VAV1 7409
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
10 PRAM-1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
11 NCK1 4690
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 PLCG1 5335
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 GRB2 2885
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 SHC1 6464
Affinity Capture-MS Homo sapiens
15 PIK3R1 5295
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
16 PTPRC 5788
Invivo Homo sapiens
17 CBL 867
Invivo Homo sapiens
18 PAG1  
Reconstituted Complex Homo sapiens
19 PTPN6 5777
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
20 SHB  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
21 WAS 7454
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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