Gene description for SHC1
Gene name SHC (Src homology 2 domain containing) transforming protein 1
Gene symbol SHC1
Other names/aliases SHC
SHCA
Species Homo sapiens
 Database cross references - SHC1
ExoCarta ExoCarta_6464
Vesiclepedia VP_6464
Entrez Gene 6464
HGNC 10840
MIM 600560
UniProt P29353  
 SHC1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Prostate cancer cells 25844599    
 Gene ontology annotations for SHC1
Molecular Function
    insulin-like growth factor receptor binding GO:0005159 IPI
    insulin receptor binding GO:0005158 IPI
    ephrin receptor binding GO:0046875 IPI
    protein tyrosine kinase activity GO:0004713 TAS
    phospholipid binding GO:0005543 TAS
    transmembrane receptor protein tyrosine kinase adaptor activity GO:0005068 TAS
    epidermal growth factor receptor binding GO:0005154 ISS
    neurotrophin TRKA receptor binding GO:0005168 IPI
    protein kinase binding GO:0019901 IBA
    protein binding GO:0005515 IPI
Biological Process
    activation of MAPK activity GO:0000187 IDA
    regulation of growth GO:0040008 IEA
    actin cytoskeleton reorganization GO:0031532 IEA
    regulation of epidermal growth factor-activated receptor activity GO:0007176 TAS
    single organismal cell-cell adhesion GO:0016337 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    insulin receptor signaling pathway GO:0008286 TAS
    Ras protein signal transduction GO:0007265 TAS
    platelet activation GO:0030168 TAS
    IRE1-mediated unfolded protein response GO:0036498 TAS
    angiogenesis GO:0001525 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    heart development GO:0007507 IEA
    innate immune response GO:0045087 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    leukocyte migration GO:0050900 TAS
    cellular protein metabolic process GO:0044267 TAS
    peptidyl-tyrosine phosphorylation GO:0018108 TAS
    MAPK cascade GO:0000165 IDA
    blood coagulation GO:0007596 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    endoplasmic reticulum unfolded protein response GO:0030968 TAS
    viral process GO:0016032 IEA
    positive regulation of DNA replication GO:0045740 ISS
    positive regulation of cell proliferation GO:0008284 NAS
Subcellular Localization
    mitochondrial matrix GO:0005759 IEA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    Shc-EGFR complex GO:0070435 ISS
 Experiment description of studies that identified SHC1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70|FLOT1
EV Enriched markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
9
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 275
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
EV Enriched markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for SHC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRAP2 9402
Affinity Capture-MS Homo sapiens
2 AXL 558
Affinity Capture-MS Homo sapiens
3 CSK 1445
Reconstituted Complex Homo sapiens
4 PRKCD 5580
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 NDP52  
Two-hybrid Homo sapiens
6 DAG1 1605
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 MME 4311
Invitro Homo sapiens
8 ABL1 25
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 FLT3  
Invitro Homo sapiens
10 EPS8 2059
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
11 RET 5979
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 APP 351
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
13 CD247 919
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
14 LRP1 4035
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
15 PTPN12  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 FCGR3A  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
17 PLCG2 5336
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 IGF1R 3480
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
19 AP2A1 160
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
20 ZAP70 7535
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
21 CALD1 800
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
22 SRC 6714
Affinity Capture-MS Homo sapiens
23 MST1R 4486
Invivo Homo sapiens
24 KDR  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 PLCG1 5335
Affinity Capture-Western Homo sapiens
26 ALK 238
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
27 EPOR  
Affinity Capture-Western Homo sapiens
28 CBLB 868
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
29 ERBB2 2064
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
30 FGFR1 2260
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
31 CEACAM1 634
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
32 MAPK1 5594
Invivo Homo sapiens
Invitro Homo sapiens
33 DDR2 4921
Invivo Homo sapiens
Two-hybrid Homo sapiens
34 PLSCR1 5359
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
35 PDGFRB 5159
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
36 ERBB3 2065
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 LYN 4067
Invitro Homo sapiens
38 CBL 867
Invitro Homo sapiens
39 CSF2RB  
Affinity Capture-Western Homo sapiens
40 PAG1 55824
Reconstituted Complex Homo sapiens
41 NTRK3 4916
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
42 FLT1 2321
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
43 CDH5  
Affinity Capture-Western Homo sapiens
44 NGFR 4804
Invivo Homo sapiens
45 NTRK1 4914
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
46 CRKL 1399
Invivo Homo sapiens
47 PTK2B 2185
Invivo Homo sapiens
Invitro Homo sapiens
48 PIK3R1 5295
Reconstituted Complex Homo sapiens
49 SHCBP1 79801
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
50 RAPGEF1 2889
Invivo Homo sapiens
51 Cd3e  
Reconstituted Complex Mus musculus
52 DOK2 9046
Affinity Capture-Western Homo sapiens
53 PTPN11 5781
Affinity Capture-MS Homo sapiens
54 CD22 933
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
55 ERBB4 2066
Affinity Capture-MS Homo sapiens
56 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
57 BCL3  
Invivo Homo sapiens
58 PTK2 5747
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
59 AP2A2 161
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
60 ITGB4 3691
Far Western Homo sapiens
61 MET 4233
Invivo Homo sapiens
62 IL2RB  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
63 PPP2R5A 5525
Invitro Homo sapiens
64 INPP5D 3635
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
65 Ptpn12 19248
Two-hybrid Mus musculus
66 MAPKAPK2 9261
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
67 GRB2 2885
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
68 INPPL1 3636
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
69 FLT4  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
70 IL2RG  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
71 IRS2 8660
Invitro Homo sapiens
72 Grap2 17444
Reconstituted Complex Mus musculus
Co-purification Mus musculus
73 SOS1 6654
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
74 FCGR2A 2212
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
75 GAB2 9846
Affinity Capture-Western Homo sapiens
76 ESR1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
77 LTK 4058
Invitro Homo sapiens
78 IL4R  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
79 DOK1 1796
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
80 LCP2 3937
Affinity Capture-MS Homo sapiens
81 EPHA2 1969
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
82 SOS2 6655
Affinity Capture-Western Homo sapiens
83 GHR  
Invivo Homo sapiens
84 CSF3R  
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
85 NTRK2 4915
Reconstituted Complex Homo sapiens
86 VAV3 10451
Affinity Capture-Western Homo sapiens
87 JAK2 3717
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
88 IRS1 3667
Reconstituted Complex Homo sapiens
89 BCR 613
Invitro Homo sapiens
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