Gene description for PIK3C2B
Gene name phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 beta
Gene symbol PIK3C2B
Other names/aliases C2-PI3K
Species Homo sapiens
 Database cross references - PIK3C2B
ExoCarta ExoCarta_5287
Vesiclepedia VP_5287
Entrez Gene 5287
HGNC 8972
MIM 602838
UniProt O00750  
 PIK3C2B identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Thymus 23844026    
Urine 15326289    
 Gene ontology annotations for PIK3C2B
Molecular Function
    1-phosphatidylinositol-3-kinase activity GO:0016303 IBA
    protein binding GO:0005515 IPI
    1-phosphatidylinositol-4-phosphate 3-kinase activity GO:0035005 NAS
    lipid kinase activity GO:0001727 IEA
    ATP binding GO:0005524 IEA
    phosphatidylinositol binding GO:0035091 IEA
Biological Process
    inflammatory response GO:0006954 IBA
    cell chemotaxis GO:0060326 IBA
    phosphatidylinositol-3-phosphate biosynthetic process GO:0036092 IBA
    protein phosphorylation GO:0006468 IBA
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    small molecule metabolic process GO:0044281 TAS
    protein kinase B signaling GO:0043491 IEA
    innate immune response GO:0045087 IBA
    platelet activation GO:0030168 IBA
    biological_process GO:0008150 ND
    phosphatidylinositol phosphorylation GO:0046854 IBA
    phospholipid metabolic process GO:0006644 TAS
    phosphatidylinositol 3-kinase signaling GO:0014065 IBA
    adaptive immune response GO:0002250 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    phosphatidylinositol 3-kinase complex GO:0005942 IBA
    endocytic vesicle GO:0030139 IEA
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    endoplasmic reticulum GO:0005783 IEA
 Experiment description of studies that identified PIK3C2B in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 13
MISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for PIK3C2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRB2 2885
Invitro Homo sapiens
2 EGFR 1956
Invitro Homo sapiens
3 SFN 2810
Affinity Capture-MS Homo sapiens
4 LTK 4058
Invivo Homo sapiens
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which PIK3C2B is involved
PathwayEvidenceSource
Synthesis of PIPs at the plasma membrane TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here