Gene description for GFAP
Gene name glial fibrillary acidic protein
Gene symbol GFAP
Other names/aliases ALXDRD
Species Homo sapiens
 Database cross references - GFAP
ExoCarta ExoCarta_2670
Vesiclepedia VP_2670
Entrez Gene 2670
HGNC 4235
MIM 137780
UniProt P14136  
 GFAP identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for GFAP
Molecular Function
    kinase binding GO:0019900 IEA
    protein binding GO:0005515 IPI
    structural constituent of cytoskeleton GO:0005200 IEA
    integrin binding GO:0005178 IEA
Biological Process
    response to wounding GO:0009611 IEA
    positive regulation of Schwann cell proliferation GO:0010625 IEA
    negative regulation of neuron projection development GO:0010977 IEA
    neuron projection regeneration GO:0031102 IEA
    astrocyte development GO:0014002 IEA
    intermediate filament organization GO:0045109 IEA
    Bergmann glial cell differentiation GO:0060020 IEA
    regulation of neurotransmitter uptake GO:0051580 IEA
    long-term synaptic potentiation GO:0060291 IEA
    extracellular matrix organization GO:0030198 IEA
Subcellular Localization
    astrocyte end-foot GO:0097450 IEA
    cytoplasm GO:0005737 IDA
    cell body GO:0044297 IEA
    cytosol GO:0005829 TAS
    intermediate filament GO:0005882 IEA
    membrane GO:0016020 IEA
 Experiment description of studies that identified GFAP in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 834
ISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
ISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix|CD81|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GFAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSEN2 5664
Two-hybrid Homo sapiens
2 TUBGCP4 27229
Two-hybrid Homo sapiens
3 RIBC2  
Two-hybrid Homo sapiens
4 GRAP2 9402
Two-hybrid Homo sapiens
5 S100B 6285
Affinity Capture-MS Homo sapiens
6 C18orf9  
Two-hybrid Homo sapiens
7 SRC 6714
Affinity Capture-MS Homo sapiens
8 MEN1 4221
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
9 S100A1 6271
Affinity Capture-MS Homo sapiens
10 PDZK1 5174
Two-hybrid Homo sapiens
11 VIM 7431
Two-hybrid Homo sapiens
12 PDLIM7 9260
Two-hybrid Homo sapiens
13 PSEN1 5663
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
14 POM121  
Two-hybrid Homo sapiens
15 PDLIM1 9124
Two-hybrid Homo sapiens
16 KIAA0408  
Two-hybrid Homo sapiens
17 MYO15B 80022
Two-hybrid Homo sapiens
18 SH3YL1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which GFAP is involved
PathwayEvidenceSource
Nuclear signaling by ERBB4 TAS Reactome





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