Gene description for GFAP
Gene name glial fibrillary acidic protein
Gene symbol GFAP
Other names/aliases ALXDRD
Species Homo sapiens
 Database cross references - GFAP
ExoCarta ExoCarta_2670
Vesiclepedia VP_2670
Entrez Gene 2670
HGNC 4235
MIM 137780
UniProt P14136  
 GFAP identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for GFAP
Molecular Function
    integrin binding GO:0005178 IEA
    structural constituent of cytoskeleton GO:0005200 IBA
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 IEA
    identical protein binding GO:0042802 IPI
Biological Process
    intracellular protein transport GO:0006886 IEA
    gene expression GO:0010467 IEA
    positive regulation of Schwann cell proliferation GO:0010625 IEA
    negative regulation of neuron projection development GO:0010977 IEA
    astrocyte development GO:0014002 IEA
    Schwann cell proliferation GO:0014010 IEA
    extracellular matrix organization GO:0030198 IEA
    neuron projection regeneration GO:0031102 IEA
    regulation of protein-containing complex assembly GO:0043254 TAS
    intermediate filament organization GO:0045109 IBA
    intermediate filament organization GO:0045109 IDA
    regulation of neurotransmitter uptake GO:0051580 IEA
    Bergmann glial cell differentiation GO:0060020 IEA
    long-term synaptic potentiation GO:0060291 IEA
    D-aspartate import across plasma membrane GO:0070779 IEA
    regulation of chaperone-mediated autophagy GO:1904714 IBA
    regulation of chaperone-mediated autophagy GO:1904714 ISS
    regulation of chaperone-mediated autophagy GO:1904714 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    lysosome GO:0005764 ISS
    cytosol GO:0005829 TAS
    intermediate filament GO:0005882 IBA
    cell projection GO:0042995 IBA
    cell body GO:0044297 IEA
    intermediate filament cytoskeleton GO:0045111 IDA
    astrocyte end-foot GO:0097450 IEA
    cytoplasmic side of lysosomal membrane GO:0098574 IEA
 Experiment description of studies that identified GFAP in exosomes
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GFAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EFNA1  
Affinity Capture-MS Homo sapiens
2 GRAP2 9402
Two-hybrid Homo sapiens
3 ENKD1  
Two-hybrid Homo sapiens
4 BPIFA1 51297
Affinity Capture-MS Homo sapiens
5 ABLIM1 3983
Proximity Label-MS Homo sapiens
6 EPM2AIP1 9852
Two-hybrid Homo sapiens
7 KRT1 3848
Proximity Label-MS Homo sapiens
8 LGALS14  
Two-hybrid Homo sapiens
9 SHC1 6464
Affinity Capture-MS Homo sapiens
10 CABS1  
Affinity Capture-MS Homo sapiens
11 C3orf58 205428
Affinity Capture-MS Homo sapiens
12 AES 166
Two-hybrid Homo sapiens
13 MYO15B 80022
Two-hybrid Homo sapiens
14 CARD10  
Proximity Label-MS Homo sapiens
15 FBXO25  
Affinity Capture-MS Homo sapiens
16 ALB 213
Affinity Capture-MS Homo sapiens
17 GFAP 2670
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 EMD 2010
Proximity Label-MS Homo sapiens
19 GOLGA6A  
Affinity Capture-MS Homo sapiens
20 C1orf198 84886
Proximity Label-MS Homo sapiens
21 POM121  
Two-hybrid Homo sapiens
22 CT55  
Two-hybrid Homo sapiens
23 PSEN2 5664
Two-hybrid Homo sapiens
24 UFD1L 7353
Proximity Label-MS Homo sapiens
25 CWF19L2  
Two-hybrid Homo sapiens
26 EFNA3  
Affinity Capture-MS Homo sapiens
27 LMO2  
Two-hybrid Homo sapiens
28 ALG3 10195
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 TNRC6A 27327
Proximity Label-MS Homo sapiens
30 SIRT2 22933
Proximity Label-MS Homo sapiens
31 KIF23 9493
Affinity Capture-MS Homo sapiens
32 FAM83B  
Proximity Label-MS Homo sapiens
33 SMPD4 55627
Proximity Label-MS Homo sapiens
34 LENG1  
Two-hybrid Homo sapiens
35 PPP1R18 170954
Two-hybrid Homo sapiens
36 SDCCAG3  
Proximity Label-MS Homo sapiens
37 HGS 9146
Reconstituted Complex Homo sapiens
38 TMEM201 199953
Proximity Label-MS Homo sapiens
39 TEKT2  
Affinity Capture-MS Homo sapiens
40 TFCP2 7024
Affinity Capture-MS Homo sapiens
41 MYCN  
Affinity Capture-MS Homo sapiens
42 WBP2 23558
Affinity Capture-MS Homo sapiens
43 SYNM 23336
Proximity Label-MS Homo sapiens
44 PDLIM1 9124
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
45 NEIL3  
Protein-RNA Homo sapiens
46 KIF14 9928
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
47 EXOC4 60412
Proximity Label-MS Homo sapiens
48 HSPA8 3312
Affinity Capture-MS Homo sapiens
49 UNC13D 201294
Proximity Label-MS Homo sapiens
50 ZC2HC1C  
Two-hybrid Homo sapiens
51 KRT8 3856
Proximity Label-MS Homo sapiens
52 KRT19 3880
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
53 IDE 3416
Affinity Capture-MS Homo sapiens
54 USP10 9100
Affinity Capture-MS Homo sapiens
55 LHB  
Affinity Capture-MS Homo sapiens
56 PRPH 5630
Proximity Label-MS Homo sapiens
57 LPP 4026
Proximity Label-MS Homo sapiens
58 LUZP1 7798
Affinity Capture-MS Homo sapiens
59 MOS 4342
Two-hybrid Homo sapiens
60 ERBB3 2065
Affinity Capture-MS Homo sapiens
61 FAM50B  
Two-hybrid Homo sapiens
62 P2RY8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 TUBGCP4 27229
Two-hybrid Homo sapiens
64 PPP1R16B  
Two-hybrid Homo sapiens
65 GOLGA2 2801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
66 PRKCG 5582
Reconstituted Complex Homo sapiens
67 KRT39  
Two-hybrid Homo sapiens
68 PAG1 55824
Affinity Capture-MS Homo sapiens
69 CCNB1 891
Affinity Capture-MS Homo sapiens
70 ESRRB  
Affinity Capture-MS Homo sapiens
71 UBASH3B 84959
Affinity Capture-MS Homo sapiens
72 CHMP4B 128866
Affinity Capture-MS Homo sapiens
73 YAP1 10413
Affinity Capture-MS Homo sapiens
74 WDYHV1  
Two-hybrid Homo sapiens
75 SIPA1L2  
Proximity Label-MS Homo sapiens
76 RORA  
Two-hybrid Homo sapiens
77 SYNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 PIAS2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
79 PDP2  
Affinity Capture-MS Homo sapiens
80 PLK1 5347
Proximity Label-MS Homo sapiens
81 ALDH3B1 221
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 ZNF655  
Two-hybrid Homo sapiens
83 MYBPHL  
Two-hybrid Homo sapiens
84 CEP76  
Two-hybrid Homo sapiens
85 TMEM131  
Proximity Label-MS Homo sapiens
86 DNM1L 10059
Affinity Capture-MS Homo sapiens
87 NFATC4 4776
Affinity Capture-MS Homo sapiens
88 INTS4P2  
Affinity Capture-MS Homo sapiens
89 UBR2 23304
Affinity Capture-MS Homo sapiens
90 BAG3 9531
Proximity Label-MS Homo sapiens
91 ANLN 54443
Affinity Capture-MS Homo sapiens
92 BIRC2  
Two-hybrid Homo sapiens
93 GAN 8139
Affinity Capture-MS Homo sapiens
94 HSPA5 3309
Affinity Capture-MS Homo sapiens
95 EFNA4  
Affinity Capture-MS Homo sapiens
96 PSEN1 5663
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
97 KIF20A 10112
Affinity Capture-MS Homo sapiens
98 ECT2 1894
Affinity Capture-MS Homo sapiens
99 SH3YL1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
100 CCDC57 284001
Two-hybrid Homo sapiens
101 NKAIN3  
Affinity Capture-MS Homo sapiens
102 CRYAB 1410
Reconstituted Complex Homo sapiens
103 NT5DC1 221294
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 HDAC9  
Affinity Capture-MS Homo sapiens
105 ZNF143  
Affinity Capture-MS Homo sapiens
106 SKP2  
Affinity Capture-MS Homo sapiens
107 DES 1674
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 IFFO1  
Proximity Label-MS Homo sapiens
109 XPNPEP3 63929
Affinity Capture-MS Homo sapiens
110 HOOK3 84376
Affinity Capture-MS Homo sapiens
111 KCNF1  
Affinity Capture-MS Homo sapiens
112 KRT27 342574
Two-hybrid Homo sapiens
113 NXF1 10482
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
114 AP2M1 1173
Affinity Capture-MS Homo sapiens
115 AURKB 9212
Biochemical Activity Homo sapiens
116 IFFO2 126917
Proximity Label-MS Homo sapiens
117 LZTS2 84445
Proximity Label-MS Homo sapiens
118 KRT15 3866
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
119 INA 9118
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 SOAT1 6646
Affinity Capture-MS Homo sapiens
121 UBR1 197131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 STAT1 6772
Two-hybrid Homo sapiens
123 NEFL 4747
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
124 NEFM 4741
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 UBASH3A 53347
Two-hybrid Homo sapiens
126 COA5  
Affinity Capture-MS Homo sapiens
127 CLEC4M  
Affinity Capture-MS Homo sapiens
128 TRIM27  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
129 RIBC2  
Two-hybrid Homo sapiens
130 KIAA1671  
Proximity Label-MS Homo sapiens
131 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 CTSF 8722
Affinity Capture-MS Homo sapiens
133 PDZK1 5174
Two-hybrid Homo sapiens
134 TMEM209 84928
Proximity Label-MS Homo sapiens
135 HEATR1 55127
Affinity Capture-MS Homo sapiens
136 KRTAP3-2  
Affinity Capture-MS Homo sapiens
137 TLR4  
Co-localization Homo sapiens
138 TCHP  
Proximity Label-MS Homo sapiens
139 SIPA1L3  
Proximity Label-MS Homo sapiens
140 DSP 1832
Proximity Label-MS Homo sapiens
141 APP 351
Reconstituted Complex Homo sapiens
142 NES 10763
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 KRT18 3875
Proximity Label-MS Homo sapiens
144 PDLIM7 9260
Two-hybrid Homo sapiens
145 UBE3A 7337
Reconstituted Complex Homo sapiens
146 TBC1D21 161514
Two-hybrid Homo sapiens
147 TP53BP2  
Two-hybrid Homo sapiens
148 ALDH3A2 224
Proximity Label-MS Homo sapiens
149 TP53 7157
Proximity Label-MS Homo sapiens
150 TMEM57  
Proximity Label-MS Homo sapiens
151 GRB2 2885
Affinity Capture-MS Homo sapiens
152 HMGB1 3146
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
153 HOMEZ  
Two-hybrid Homo sapiens
154 NEK6 10783
Two-hybrid Homo sapiens
155 PDCD6IP 10015
Affinity Capture-Western Homo sapiens
156 PSMC4 5704
Proximity Label-MS Homo sapiens
157 MEN1 4221
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
158 RSPRY1 89970
Affinity Capture-MS Homo sapiens
159 IKBKG 8517
Two-hybrid Homo sapiens
160 HLTF  
Affinity Capture-MS Homo sapiens
161 Htt  
Affinity Capture-MS Mus musculus
162 TRIM9  
Affinity Capture-MS Homo sapiens
163 GBA 2629
Affinity Capture-MS Homo sapiens
164 NUDT19 390916
Proximity Label-MS Homo sapiens
165 FAM83H 286077
Proximity Label-MS Homo sapiens
166 DDX58 23586
Affinity Capture-RNA Homo sapiens
167 CDK1 983
Affinity Capture-MS Homo sapiens
168 NFKBID  
Two-hybrid Homo sapiens
169 COQ2  
Affinity Capture-MS Homo sapiens
170 CEBPA  
Affinity Capture-MS Homo sapiens
171 ABI2 10152
Two-hybrid Homo sapiens
172 PIH1D2  
Two-hybrid Homo sapiens
173 CEP152  
Proximity Label-MS Homo sapiens
174 ERRFI1 54206
Affinity Capture-MS Homo sapiens
175 UBALD2  
Affinity Capture-MS Homo sapiens
176 LBX2  
Affinity Capture-MS Homo sapiens
177 ZUFSP 221302
Affinity Capture-MS Homo sapiens
178 EHMT1  
Co-localization Homo sapiens
179 KRT13 3860
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
180 C6orf165  
Two-hybrid Homo sapiens
181 YES1 7525
Two-hybrid Homo sapiens
182 ATOH1  
Affinity Capture-MS Homo sapiens
183 VIM 7431
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
184 ZNF774  
Two-hybrid Homo sapiens
185 KIAA0408  
Two-hybrid Homo sapiens
186 CDC37 11140
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
187 IARS2 55699
Proximity Label-MS Homo sapiens
188 DNAH14  
Affinity Capture-MS Homo sapiens
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