Gene description for UFD1L
Gene name ubiquitin fusion degradation 1 like (yeast)
Gene symbol UFD1L
Other names/aliases UFD1
Species Homo sapiens
 Database cross references - UFD1L
ExoCarta ExoCarta_7353
Entrez Gene 7353
HGNC 12520
MIM 601754
UniProt Q92890  
 UFD1L identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for UFD1L
Molecular Function
    ubiquitin-specific protease activity GO:0004843 TAS
    receptor binding GO:0005102 IEA
    protein binding GO:0005515 IPI
    protein complex binding GO:0032403 IEA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 TAS
    translesion synthesis GO:0019985 TAS
    error-free translesion synthesis GO:0070987 TAS
    DNA repair GO:0006281 TAS
    ER-associated misfolded protein catabolic process GO:0071712 IMP
    skeletal system development GO:0001501 TAS
    retrograde protein transport, ER to cytosol GO:0030970 IMP
Subcellular Localization
    VCP-NPL4-UFD1 AAA ATPase complex GO:0034098 ISS
    cytosol GO:0005829 IEA
    nucleus GO:0005634 IDA
    protein complex GO:0043234 IPI
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified UFD1L in exosomes
1
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UFD1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SELS  
Affinity Capture-Western Homo sapiens
2 NPLOC4 55666
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which UFD1L is involved
PathwayEvidenceSource
Translesion Synthesis by POLH TAS Reactome





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