Gene description for DPH5
Gene name diphthamide biosynthesis 5
Gene symbol DPH5
Other names/aliases AD-018
CGI-30
HSPC143
NPD015
Species Homo sapiens
 Database cross references - DPH5
ExoCarta ExoCarta_51611
Vesiclepedia VP_51611
Entrez Gene 51611
HGNC 24270
MIM 611075
UniProt Q9H2P9  
 DPH5 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DPH5
Molecular Function
    diphthine synthase activity GO:0004164 NAS
Biological Process
    methylation GO:0032259 IEA
    peptidyl-diphthamide biosynthetic process from peptidyl-histidine GO:0017183 TAS
    cellular protein metabolic process GO:0044267 TAS
    post-translational protein modification GO:0043687 TAS
Subcellular Localization
    cellular_component GO:0005575 ND
    cytosol GO:0005829 TAS
 Experiment description of studies that identified DPH5 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DPH5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which DPH5 is involved
PathwayEvidenceSource
Synthesis of diphthamide-EEF2 TAS Reactome





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