Gene description for MTMR12
Gene name myotubularin related protein 12
Gene symbol MTMR12
Other names/aliases 3-PAP
PIP3AP
Species Homo sapiens
 Database cross references - MTMR12
ExoCarta ExoCarta_54545
Vesiclepedia VP_54545
Entrez Gene 54545
HGNC 18191
MIM 606501
UniProt Q9C0I1  
 MTMR12 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for MTMR12
Molecular Function
    phosphatidylinositol-3-phosphate phosphatase activity GO:0004438 IBA
    protein binding GO:0005515 IPI
Biological Process
    phosphatidylinositol dephosphorylation GO:0046856 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IBA
    sarcoplasmic reticulum GO:0016529 IEA
    sarcomere GO:0030017 IEA
 Experiment description of studies that identified MTMR12 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MTMR12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNX27 81609
Affinity Capture-MS Homo sapiens
2 CGA  
Affinity Capture-MS Homo sapiens
3 TIMM21  
Affinity Capture-MS Homo sapiens
4 DUSP1  
Affinity Capture-MS Homo sapiens
5 NPLOC4 55666
Co-fractionation Homo sapiens
6 GMPPB 29925
Affinity Capture-MS Homo sapiens
7 Mtmr2  
Affinity Capture-MS Mus musculus
8 VAPB 9217
Affinity Capture-MS Homo sapiens
9 VAPA 9218
Affinity Capture-MS Homo sapiens
10 NDUFA13 51079
Affinity Capture-MS Homo sapiens
11 YWHAB 7529
Affinity Capture-MS Homo sapiens
12 PARD3 56288
Proximity Label-MS Homo sapiens
13 MAPK3 5595
Co-fractionation Homo sapiens
14 UFD1L 7353
Co-fractionation Homo sapiens
15 MTMR12 54545
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 NDUFS8 4728
Affinity Capture-MS Homo sapiens
17 RPA3 6119
Proximity Label-MS Homo sapiens
18 HAUS8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 RTCB 51493
Co-fractionation Homo sapiens
20 S100B 6285
Affinity Capture-MS Homo sapiens
21 AKR7A2 8574
Affinity Capture-MS Homo sapiens
22 SCO1  
Affinity Capture-MS Homo sapiens
23 CYC1 1537
Affinity Capture-MS Homo sapiens
24 MTMR2 8898
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SFXN4  
Affinity Capture-MS Homo sapiens
26 THEM6  
Affinity Capture-MS Homo sapiens
27 NPPC  
Affinity Capture-MS Homo sapiens
28 SCO2  
Affinity Capture-MS Homo sapiens
29 HLA-C 3107
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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