Gene description for NGFR
Gene name nerve growth factor receptor
Gene symbol NGFR
Other names/aliases CD271
Gp80-LNGFR
TNFRSF16
p75(NTR)
p75NTR
Species Homo sapiens
 Database cross references - NGFR
ExoCarta ExoCarta_4804
Vesiclepedia VP_4804
Entrez Gene 4804
HGNC 7809
MIM 162010
UniProt P08138  
 NGFR identified in sEVs derived from the following tissue/cell type
Melanocytes 34957415    
Melanoma cells 25950383    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
 Gene ontology annotations for NGFR
Molecular Function
    amyloid-beta binding GO:0001540 TAS
    transmembrane signaling receptor activity GO:0004888 TAS
    death receptor activity GO:0005035 IBA
    death receptor activity GO:0005035 IGI
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IDA
    coreceptor activity GO:0015026 IBA
    coreceptor activity GO:0015026 IDA
    small GTPase binding GO:0031267 ISS
    ubiquitin protein ligase binding GO:0031625 IPI
    signaling receptor activity GO:0038023 TAS
    neurotrophin binding GO:0043121 ISS
    nerve growth factor binding GO:0048406 IBA
    nerve growth factor binding GO:0048406 ISS
Biological Process
    intracellular glucose homeostasis GO:0001678 ISS
    intracellular protein transport GO:0006886 ISS
    Rho protein signal transduction GO:0007266 IBA
    Rho protein signal transduction GO:0007266 IDA
    axon guidance GO:0007411 IEA
    central nervous system development GO:0007417 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    detection of temperature stimulus GO:0016048 IEA
    nerve development GO:0021675 IEA
    negative regulation of cell migration GO:0030336 IMP
    hair follicle morphogenesis GO:0031069 IEA
    circadian regulation of gene expression GO:0032922 ISS
    dorsal aorta development GO:0035907 IEA
    negative regulation of fibroblast growth factor receptor signaling pathway GO:0040037 IEA
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    positive regulation of odontogenesis of dentin-containing tooth GO:0042488 IEA
    glucose homeostasis GO:0042593 ISS
    fibroblast proliferation GO:0048144 IEA
    positive regulation of fibroblast proliferation GO:0048146 IEA
    neuron apoptotic process GO:0051402 IGI
    negative regulation of hair follicle development GO:0051799 IEA
    extrinsic apoptotic signaling pathway GO:0097191 IDA
    presynaptic modulation of chemical synaptic transmission GO:0099171 IEA
    positive regulation of protein localization to nucleus GO:1900182 IDA
    positive regulation of miRNA transcription GO:1902895 IDA
    negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis GO:1903588 IGI
    cellular response to amyloid-beta GO:1904646 IDA
    positive regulation of endothelial cell apoptotic process GO:2000353 IMP
    positive regulation of apoptotic signaling pathway GO:2001235 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    endosome GO:0005768 TAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IEA
    cell surface GO:0009986 IBA
    cell surface GO:0009986 ISS
    postsynaptic density GO:0014069 IEA
    membrane GO:0016020 NAS
    growth cone GO:0030426 IEA
    neuromuscular junction GO:0031594 IEA
    dendritic spine GO:0043197 IEA
    perikaryon GO:0043204 IEA
    presynapse GO:0098793 IEA
 Experiment description of studies that identified NGFR in sEVs
1
Experiment ID 1110
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Homo sapiens
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanocytes
Sample name Melano3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 259
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1107
MISEV standards
EM
Biophysical techniques
CD63|CD81|GAPDH
Enriched markers
GOLGA2|CANX
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
Flow cytometry
PubMed ID 34957415    
Organism Homo sapiens
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name SK-MEL-147
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Flow cytometry
4
Experiment ID 1113
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
Flow cytometry
PubMed ID 34957415    
Organism Homo sapiens
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name SK-MEL-28
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Flow cytometry
5
Experiment ID 1114
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Homo sapiens
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name SK-MEL-103
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
6
Experiment ID 1115
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Homo sapiens
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name SK-MEL-147
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
7
Experiment ID 1116
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Homo sapiens
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name SK-MEL-147
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
 Protein-protein interactions for NGFR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BFAR  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
2 UFD1L 7353
Two-hybrid Homo sapiens
3 TRAF2 7186
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 SMN1 6606
Two-hybrid Homo sapiens
5 RPN2 6185
Two-hybrid Homo sapiens
6 SHC1 6464
Affinity Capture-Western Homo sapiens
7 NGF  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
8 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
9 STMN2  
Two-hybrid Homo sapiens
10 TRAF4 9618
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
11 PDLIM1 9124
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 TTR 7276
Two-hybrid Homo sapiens
13 ANXA7 310
Two-hybrid Homo sapiens
14 TRAF6 7189
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
15 TRADD 8717
Affinity Capture-Western Homo sapiens
16 NDNL2 56160
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
17 CDKN1A  
Two-hybrid Homo sapiens
18 TRAF3 7187
Affinity Capture-Western Homo sapiens
19 APP 351
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
20 TCF19  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
21 TRIM37  
Reconstituted Complex Homo sapiens
22 PRKACB 5567
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
23 MAGED1 9500
Affinity Capture-Western Homo sapiens
24 MAGEH1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 NGFR 4804
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
26 NTRK1 4914
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 TRAF5  
Affinity Capture-Western Homo sapiens
28 FSCN1 6624
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
29 CAV1 857
Reconstituted Complex Homo sapiens
30 TRAF1 7185
Affinity Capture-Western Homo sapiens
31 NDN 4692
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 MDM2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
33 RCC1 1104
Two-hybrid Homo sapiens
34 H2AFX 3014
Two-hybrid Homo sapiens
35 TK1 7083
Two-hybrid Homo sapiens
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