Gene description for NDN
Gene name necdin, melanoma antigen (MAGE) family member
Gene symbol NDN
Other names/aliases HsT16328
PWCR
Species Homo sapiens
 Database cross references - NDN
ExoCarta ExoCarta_4692
Vesiclepedia VP_4692
Entrez Gene 4692
HGNC 7675
MIM 602117
UniProt Q99608  
 NDN identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NDN
Molecular Function
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    gamma-tubulin binding GO:0043015 IEA
    promoter-specific chromatin binding GO:1990841 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IBA
    neuron migration GO:0001764 IEA
    respiratory system process GO:0003016 IEA
    axonal fasciculation GO:0007413 IEA
    central nervous system development GO:0007417 IEA
    glial cell migration GO:0008347 IEA
    post-embryonic development GO:0009791 IEA
    sensory perception of pain GO:0019233 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 IEA
    axon extension GO:0048675 IEA
    multicellular organismal-level homeostasis GO:0048871 IEA
    genomic imprinting GO:0071514 IEA
    positive regulation of protein deacetylation GO:0090312 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    centrosome GO:0005813 IEA
    cytosol GO:0005829 IDA
    protein-containing complex GO:0032991 IEA
    cell projection GO:0042995 IEA
    perikaryon GO:0043204 IEA
 Experiment description of studies that identified NDN in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NDN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NUCB2 4925
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 P4HA3 283208
Two-hybrid Homo sapiens
3 FSD2 123722
Two-hybrid Homo sapiens
4 RPS11 6205
Affinity Capture-MS Homo sapiens
5 BLOC1S6  
Two-hybrid Homo sapiens
6 TFIP11  
Two-hybrid Homo sapiens
7 TP53 7157
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 FAM131C  
Two-hybrid Homo sapiens
9 DHCR7 1717
Affinity Capture-MS Homo sapiens
10 PPFIA1 8500
Two-hybrid Homo sapiens
11 SRPRB 58477
Affinity Capture-MS Homo sapiens
12 LAX1  
Two-hybrid Homo sapiens
13 EPAS1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
14 IL32  
Two-hybrid Homo sapiens
15 EID2  
Affinity Capture-Western Homo sapiens
16 E2F1 1869
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 ARSE 415
Two-hybrid Homo sapiens
18 ZBTB1  
Two-hybrid Homo sapiens
19 PPARGC1A  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
20 IFFO1  
Two-hybrid Homo sapiens
21 EGFR 1956
PCA Homo sapiens
22 TNPO1 3842
Affinity Capture-Western Homo sapiens
23 U2AF1 7307
Two-hybrid Homo sapiens
24 SIRT1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
25 LPXN 9404
Two-hybrid Homo sapiens
26 GOLGA2 2801
Two-hybrid Homo sapiens
27 AUP1 550
Affinity Capture-MS Homo sapiens
28 CERS2 29956
Affinity Capture-MS Homo sapiens
29 EID2B  
Affinity Capture-Western Homo sapiens
30 DTNBP1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 TRIM28 10155
Affinity Capture-MS Homo sapiens
32 MKRN2 23609
Two-hybrid Homo sapiens
33 NEFL 4747
Two-hybrid Homo sapiens
34 MYC  
Affinity Capture-MS Homo sapiens
35 GKAP1  
Two-hybrid Homo sapiens
36 FXR2 9513
Two-hybrid Homo sapiens
37 NGFR 4804
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
38 EID3  
Affinity Capture-Western Homo sapiens
39 SSX2IP  
Two-hybrid Homo sapiens
40 CDR2  
Two-hybrid Homo sapiens
41 ZBTB7B  
Two-hybrid Homo sapiens
42 NUBP1 4682
Two-hybrid Homo sapiens
43 KPNB1 3837
Affinity Capture-Western Homo sapiens
44 EID1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
45 MPP6 51678
Reconstituted Complex Homo sapiens
46 HNRNPU 3192
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
47 CCDC136  
Two-hybrid Homo sapiens
48 NSMCE4A  
Affinity Capture-Western Homo sapiens
49 CALCOCO2  
Two-hybrid Homo sapiens
50 MIER2  
Two-hybrid Homo sapiens
51 N6AMT2 221143
Two-hybrid Homo sapiens
52 TMEM33 55161
Affinity Capture-MS Homo sapiens
53 NDN 4692
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
54 APBB1IP 54518
Two-hybrid Homo sapiens
55 SERTAD3  
Two-hybrid Homo sapiens
56 IL1A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
57 SEC61A1 29927
Affinity Capture-MS Homo sapiens
58 HIF1A 3091
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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