Gene description for AUP1
Gene name ancient ubiquitous protein 1
Gene symbol AUP1
Other names/aliases -
Species Homo sapiens
 Database cross references - AUP1
ExoCarta ExoCarta_550
Vesiclepedia VP_550
Entrez Gene 550
HGNC 891
MIM 602434
UniProt Q9Y679  
 AUP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for AUP1
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin conjugating enzyme binding GO:0031624 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    ubiquitin binding GO:0043130 IEA
Biological Process
    response to virus GO:0009615 IMP
    retrograde protein transport, ER to cytosol GO:0030970 IMP
    lipid droplet organization GO:0034389 IDA
    ERAD pathway GO:0036503 IBA
    ERAD pathway GO:0036503 IMP
    lipophagy GO:0061724 IMP
    lipid droplet formation GO:0140042 IMP
    protein localization to lipid droplet GO:1990044 IDA
    protein localization to lipid droplet GO:1990044 IMP
Subcellular Localization
    autophagosome GO:0005776 IDA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    lipid droplet GO:0005811 IDA
    membrane GO:0016020 HDA
    cytoplasmic vesicle GO:0031410 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified AUP1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for AUP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RAMP3  
Affinity Capture-MS Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 ITGA2B 3674
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
5 UBL4A 8266
Affinity Capture-MS Homo sapiens
6 METTL7A 25840
Proximity Label-MS Homo sapiens
7 LAMP3  
Proximity Label-MS Homo sapiens
8 OS9 10956
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
9 GJA1 2697
Proximity Label-MS Homo sapiens
10 OPRD1  
Affinity Capture-Western Homo sapiens
11 ATP12A 479
Affinity Capture-MS Homo sapiens
12 SLC2A1 6513
Affinity Capture-MS Homo sapiens
13 SURF4 6836
Affinity Capture-MS Homo sapiens
14 SPNS1 83985
Affinity Capture-MS Homo sapiens
15 HSD17B11 51170
Proximity Label-MS Homo sapiens
16 UBC 7316
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 LAMP2 3920
Proximity Label-MS Homo sapiens
18 DNAJC25 548645
Proximity Label-MS Homo sapiens
19 PTPN1 5770
Proximity Label-MS Homo sapiens
20 SYVN1 84447
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 STX4 6810
Proximity Label-MS Homo sapiens
22 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
23 FFAR1  
Affinity Capture-MS Homo sapiens
24 CAPRIN2  
Affinity Capture-MS Homo sapiens
25 LPCAT1 79888
Affinity Capture-MS Homo sapiens
26 UBE2G2  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
27 S1PR4  
Affinity Capture-MS Homo sapiens
28 Kcnk1  
Affinity Capture-MS Mus musculus
29 PTP4A1 7803
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 FNTB 2342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 RAB3B 5865
Proximity Label-MS Homo sapiens
32 SEL1L 6400
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
33 OPRM1 4988
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
34 STX7 8417
Proximity Label-MS Homo sapiens
35 MDC1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
36 GPR55  
Affinity Capture-MS Homo sapiens
37 SSR1 6745
Proximity Label-MS Homo sapiens
38 DDOST 1650
Affinity Capture-MS Homo sapiens
39 SLC25A46 91137
Proximity Label-MS Homo sapiens
40 F2RL1  
Affinity Capture-MS Homo sapiens
41 KPTN  
Affinity Capture-MS Homo sapiens
42 ATP13A3 79572
Affinity Capture-MS Homo sapiens
43 FAF2 23197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
44 FAM20C 56975
Affinity Capture-MS Homo sapiens
45 MARCKS 4082
Proximity Label-MS Homo sapiens
46 RNF11 26994
Two-hybrid Homo sapiens
47 ATP1A3 478
Affinity Capture-MS Homo sapiens
48 LMAN1 3998
Proximity Label-MS Homo sapiens
49 NUP155 9631
Proximity Label-MS Homo sapiens
50 KIAA1715 80856
Proximity Label-MS Homo sapiens
51 PXMP2  
Proximity Label-MS Homo sapiens
52 RAB5A 5868
Proximity Label-MS Homo sapiens
53 TMEM248 55069
Affinity Capture-MS Homo sapiens
54 LMNB1 4001
Proximity Label-MS Homo sapiens
55 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
56 OPRK1  
Affinity Capture-Western Homo sapiens
57 EMD 2010
Proximity Label-MS Homo sapiens
58 AMFR 267
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
59 TMEM159 57146
Affinity Capture-MS Homo sapiens
60 P2RY8  
Affinity Capture-MS Homo sapiens
61 TSPAN3 10099
Affinity Capture-MS Homo sapiens
62 GPR35  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
63 TUBA1C 84790
Affinity Capture-MS Homo sapiens
64 VCP 7415
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
65 NTRK1 4914
Affinity Capture-MS Homo sapiens
66 GPR182  
Affinity Capture-MS Homo sapiens
67 APOB 338
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 RPN1 6184
Proximity Label-MS Homo sapiens
69 PRKDC 5591
Affinity Capture-MS Homo sapiens
70 RHOT2 89941
Proximity Label-MS Homo sapiens
71 ELOVL5 60481
Proximity Label-MS Homo sapiens
72 CMKLR1 1240
Affinity Capture-MS Homo sapiens
73 SEC61B 10952
Proximity Label-MS Homo sapiens
74 RMDN3 55177
Proximity Label-MS Homo sapiens
75 APLNR  
Affinity Capture-MS Homo sapiens
76 REEP5 7905
Proximity Label-MS Homo sapiens
77 SEC63 11231
Proximity Label-MS Homo sapiens
78 TAP1 6890
Affinity Capture-MS Homo sapiens
79 CKAP5 9793
Affinity Capture-MS Homo sapiens
80 DIP2B 57609
Affinity Capture-MS Homo sapiens
81 VIPR2  
Affinity Capture-MS Homo sapiens
82 SPTLC1 10558
Affinity Capture-MS Homo sapiens
83 CLN3 1201
Affinity Capture-MS Homo sapiens
84 AGPAT6 137964
Affinity Capture-MS Homo sapiens
85 RPN2 6185
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 CUL3 8452
Affinity Capture-MS Homo sapiens
87 CANX 821
Affinity Capture-MS Homo sapiens
88 KCND3 3752
Affinity Capture-Western Homo sapiens
89 LRRC59 55379
Proximity Label-MS Homo sapiens
90 PANX1 24145
Proximity Label-MS Homo sapiens
91 LAMP1 3916
Proximity Label-MS Homo sapiens
92 Bmpr1a  
Affinity Capture-MS Mus musculus
93 ATG12  
Affinity Capture-MS Homo sapiens
94 BCAP31 10134
Proximity Label-MS Homo sapiens
95 HSD3B7 80270
Proximity Label-MS Homo sapiens
96 GPR12  
Affinity Capture-MS Homo sapiens
97 NR3C1 2908
Proximity Label-MS Homo sapiens
98 STIM1 6786
Proximity Label-MS Homo sapiens
99 EGFR 1956
Negative Genetic Homo sapiens
100 FKBP8 23770
Proximity Label-MS Homo sapiens
101 ARF6 382
Proximity Label-MS Homo sapiens
102 FBXO6 26270
Affinity Capture-MS Homo sapiens
103 AQP3  
Affinity Capture-MS Homo sapiens
104 RAB35 11021
Proximity Label-MS Homo sapiens
105 ITGA5 3678
Two-hybrid Homo sapiens
106 ITGA1 3672
Two-hybrid Homo sapiens
107 ACSF3 197322
Affinity Capture-MS Homo sapiens
108 FASN 2194
Negative Genetic Homo sapiens
109 Nup107  
Affinity Capture-MS Mus musculus
110 RPA3 6119
Proximity Label-MS Homo sapiens
111 ATP2A1 487
Proximity Label-MS Homo sapiens
112 GPR45  
Affinity Capture-MS Homo sapiens
113 RAB9A 9367
Proximity Label-MS Homo sapiens
114 DHFRL1  
Proximity Label-MS Homo sapiens
115 SLC12A6 9990
Affinity Capture-MS Homo sapiens
116 ILK 3611
Affinity Capture-MS Homo sapiens
117 RNF114 55905
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 RNF26  
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
119 LMNA 4000
Proximity Label-MS Homo sapiens
120 PEX3 8504
Proximity Label-MS Homo sapiens
121 AKAP1 8165
Proximity Label-MS Homo sapiens
122 ITGAM 3684
Two-hybrid Homo sapiens
123 HUWE1 10075
Affinity Capture-MS Homo sapiens
124 ACBD5 91452
Proximity Label-MS Homo sapiens
125 UBE2J1 51465
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
126 HMGCR  
Affinity Capture-Western Homo sapiens
127 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
128 FBXW11  
Affinity Capture-MS Homo sapiens
129 FAM175B 23172
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
130 ABCB6 10058
Affinity Capture-MS Homo sapiens
131 KIF14 9928
Affinity Capture-MS Homo sapiens
132 TUBG1 7283
Affinity Capture-MS Homo sapiens
133 MFSD4  
Affinity Capture-MS Homo sapiens
134 EDEM1  
Affinity Capture-MS Homo sapiens
135 PCMTD2  
Affinity Capture-MS Homo sapiens
136 NUP35 129401
Proximity Label-MS Homo sapiens
137 ERGIC1 57222
Proximity Label-MS Homo sapiens
138 PTPRO 5800
Affinity Capture-MS Homo sapiens
139 ITGA2 3673
Two-hybrid Homo sapiens
140 ITGAV 3685
Two-hybrid Homo sapiens
141 ERGIC2 51290
Proximity Label-MS Homo sapiens
142 SEC62 7095
Proximity Label-MS Homo sapiens
143 B3GAT1  
Proximity Label-MS Homo sapiens
144 C16orf72 29035
Affinity Capture-MS Homo sapiens
145 RAB7A 7879
Proximity Label-MS Homo sapiens
146 RAB11A 8766
Proximity Label-MS Homo sapiens
147 LPAR2 9170
Affinity Capture-MS Homo sapiens
148 GRSF1 2926
Two-hybrid Homo sapiens
149 STT3B 201595
Affinity Capture-Western Homo sapiens
150 DNAJC1 64215
Proximity Label-MS Homo sapiens
151 VIPR1  
Affinity Capture-MS Homo sapiens
152 RAB5C 5878
Proximity Label-MS Homo sapiens
153 C9orf72  
Affinity Capture-MS Homo sapiens
154 CKAP4 10970
Proximity Label-MS Homo sapiens
155 SLC1A1 6505
Affinity Capture-MS Homo sapiens
156 NDN 4692
Affinity Capture-MS Homo sapiens
157 EP300 2033
Affinity Capture-MS Homo sapiens
158 DERL1 79139
Proximity Label-MS Homo sapiens
159 RNF139  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which AUP1 is involved
No pathways found





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