Gene description for SPNS1
Gene name spinster homolog 1 (Drosophila)
Gene symbol SPNS1
Other names/aliases HSpin1
LAT
PP2030
SPIN1
SPINL
nrs
Species Homo sapiens
 Database cross references - SPNS1
ExoCarta ExoCarta_83985
Vesiclepedia VP_83985
Entrez Gene 83985
HGNC 30621
MIM 612583
UniProt Q9H2V7  
 SPNS1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SPNS1
Molecular Function
    protein binding GO:0005515 IPI
    transmembrane transporter activity GO:0022857 IBA
Biological Process
    phospholipid efflux GO:0033700 IBA
    regulation of lysosomal lumen pH GO:0035751 IEA
    lysophospholipid transport GO:0051977 IBA
    transmembrane transport GO:0055085 IEA
Subcellular Localization
    mitochondrial inner membrane GO:0005743 IEA
    lysosomal membrane GO:0005765 HDA
    membrane GO:0016020 IBA
 Experiment description of studies that identified SPNS1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 254
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1|CD81
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 255
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 256
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 257
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SPNS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAMP3  
Affinity Capture-MS Homo sapiens
2 Cep152  
Affinity Capture-MS Mus musculus
3 SEC63 11231
Affinity Capture-MS Homo sapiens
4 Rab5c 19345
Affinity Capture-MS Mus musculus
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 FASN 2194
Negative Genetic Homo sapiens
7 CYB5B 80777
Co-fractionation Homo sapiens
8 KLK15  
Affinity Capture-MS Homo sapiens
9 CA10  
Affinity Capture-MS Homo sapiens
10 RRBP1 6238
Affinity Capture-MS Homo sapiens
11 COIL  
Proximity Label-MS Homo sapiens
12 LAMTOR1 55004
Proximity Label-MS Homo sapiens
13 SYNGR1 9145
Affinity Capture-MS Homo sapiens
14 OPALIN  
Affinity Capture-MS Homo sapiens
15 TNFSF8  
Affinity Capture-MS Homo sapiens
16 HLA-B 3106
Affinity Capture-MS Homo sapiens
17 NPTN 27020
Co-fractionation Homo sapiens
18 AUP1 550
Affinity Capture-MS Homo sapiens
19 CLEC2D  
Affinity Capture-MS Homo sapiens
20 CUL3 8452
Affinity Capture-MS Homo sapiens
21 MECP2 4204
Affinity Capture-MS Homo sapiens
22 TP53 7157
Affinity Capture-MS Homo sapiens
23 LAMP2 3920
Proximity Label-MS Homo sapiens
24 UNC93B1 81622
Affinity Capture-MS Homo sapiens
25 ARF6 382
Proximity Label-MS Homo sapiens
26 HTR2C  
Two-hybrid Homo sapiens
27 AQP3  
Affinity Capture-MS Homo sapiens
28 P2RY8  
Affinity Capture-MS Homo sapiens
29 KLRB1  
Affinity Capture-MS Homo sapiens
30 NLRX1 79671
Affinity Capture-MS Homo sapiens
31 VSIG4  
Affinity Capture-MS Homo sapiens
32 GZMH  
Affinity Capture-MS Homo sapiens
33 ARMC6 93436
Affinity Capture-MS Homo sapiens
34 CCR9  
Two-hybrid Homo sapiens
35 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
36 PLD3 23646
Affinity Capture-MS Homo sapiens
37 BCL2  
Affinity Capture-Western Homo sapiens
38 RPA3 6119
Proximity Label-MS Homo sapiens
39 IL12RB1  
Affinity Capture-MS Homo sapiens
40 SPPL2B 56928
Affinity Capture-MS Homo sapiens
41 CHRM5  
Two-hybrid Homo sapiens
42 GPR182  
Affinity Capture-MS Homo sapiens
43 BCL2L1 598
Affinity Capture-Western Homo sapiens
44 ADORA2A  
Two-hybrid Homo sapiens
45 BDKRB2  
Two-hybrid Homo sapiens
46 GPRC5B 51704
Affinity Capture-MS Homo sapiens
47 RC3H1 149041
Affinity Capture-MS Homo sapiens
48 MAP3K15  
Affinity Capture-MS Homo sapiens
49 CLEC4E  
Affinity Capture-MS Homo sapiens
50 FFAR1  
Affinity Capture-MS Homo sapiens
51 CCR8  
Two-hybrid Homo sapiens
52 TMEM192 201931
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SPNS1 is involved
No pathways found





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