Gene description for AGPAT6
Gene name 1-acylglycerol-3-phosphate O-acyltransferase 6
Gene symbol AGPAT6
Other names/aliases 1-AGPAT 6
LPAAT-zeta
LPAATZ
TSARG7
Species Homo sapiens
 Database cross references - AGPAT6
ExoCarta ExoCarta_137964
Entrez Gene 137964
HGNC 20880
MIM 608143
UniProt Q86UL3  
 AGPAT6 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for AGPAT6
Molecular Function
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 IDA
    glycerol-3-phosphate O-acyltransferase activity GO:0004366 IDA
Biological Process
    diacylglycerol metabolic process GO:0046339 IEA
    lipid biosynthetic process GO:0008610 ISS
    triglyceride biosynthetic process GO:0019432 TAS
    phosphatidic acid biosynthetic process GO:0006654 TAS
    CDP-diacylglycerol biosynthetic process GO:0016024 IEA
    fatty acid metabolic process GO:0006631 IEA
    glandular epithelial cell maturation GO:0002071 IEA
    glycerophospholipid biosynthetic process GO:0046474 TAS
    phosphatidylcholine biosynthetic process GO:0006656 IDA
    regulation of multicellular organism growth GO:0040014 IEA
    cellular lipid metabolic process GO:0044255 TAS
    small molecule metabolic process GO:0044281 TAS
    lactation GO:0007595 ISS
    phospholipid metabolic process GO:0006644 TAS
    acyl-CoA metabolic process GO:0006637 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 ISS
    integral component of membrane GO:0016021 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 IDA
 Experiment description of studies that identified AGPAT6 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for AGPAT6
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which AGPAT6 is involved
PathwayEvidenceSource
Synthesis of PA TAS Reactome
Triglyceride Biosynthesis TAS Reactome





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