Gene description for AGPAT6
Gene name 1-acylglycerol-3-phosphate O-acyltransferase 6
Gene symbol AGPAT6
Other names/aliases 1-AGPAT 6
LPAAT-zeta
LPAATZ
TSARG7
Species Homo sapiens
 Database cross references - AGPAT6
ExoCarta ExoCarta_137964
Vesiclepedia VP_137964
Entrez Gene 137964
HGNC 20880
MIM 608143
UniProt Q86UL3  
 AGPAT6 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for AGPAT6
Molecular Function
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 IDA
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 TAS
    glycerol-3-phosphate O-acyltransferase activity GO:0004366 IDA
    glycerol-3-phosphate O-acyltransferase activity GO:0004366 TAS
Biological Process
    glandular epithelial cell maturation GO:0002071 IEA
    fatty acid metabolic process GO:0006631 IEA
    acyl-CoA metabolic process GO:0006637 IDA
    phosphatidic acid biosynthetic process GO:0006654 TAS
    phosphatidylcholine biosynthetic process GO:0006656 IDA
    lactation GO:0007595 ISS
    lipid biosynthetic process GO:0008610 ISS
    CDP-diacylglycerol biosynthetic process GO:0016024 IEA
    triglyceride biosynthetic process GO:0019432 IDA
    triglyceride biosynthetic process GO:0019432 ISS
    regulation of multicellular organism growth GO:0040014 IEA
    diacylglycerol metabolic process GO:0046339 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    membrane GO:0016020 NAS
 Experiment description of studies that identified AGPAT6 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
7
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for AGPAT6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STARD3NL 83930
Affinity Capture-MS Homo sapiens
2 RAMP3  
Affinity Capture-MS Homo sapiens
3 HSD17B11 51170
Proximity Label-MS Homo sapiens
4 TSPAN17 26262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 MSH6 2956
Affinity Capture-MS Homo sapiens
6 METTL7A 25840
Proximity Label-MS Homo sapiens
7 TMEM184A  
Affinity Capture-MS Homo sapiens
8 GJD3  
Proximity Label-MS Homo sapiens
9 DNAJC25 548645
Proximity Label-MS Homo sapiens
10 PTPN1 5770
Proximity Label-MS Homo sapiens
11 STX4 6810
Proximity Label-MS Homo sapiens
12 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
13 FFAR1  
Affinity Capture-MS Homo sapiens
14 B3GAT1  
Proximity Label-MS Homo sapiens
15 CHP1 11261
Affinity Capture-MS Homo sapiens
16 HLA-B 3106
Affinity Capture-MS Homo sapiens
17 SSR1 6745
Proximity Label-MS Homo sapiens
18 EHD2 30846
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ATP2A3 489
Affinity Capture-MS Homo sapiens
20 TTYH1  
Affinity Capture-MS Homo sapiens
21 DFNA5 1687
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 FEM1A  
Affinity Capture-MS Homo sapiens
23 GFI1B  
Two-hybrid Homo sapiens
24 MARCKS 4082
Proximity Label-MS Homo sapiens
25 ATP1A3 478
Affinity Capture-MS Homo sapiens
26 VSIG4  
Affinity Capture-MS Homo sapiens
27 KIAA1715 80856
Proximity Label-MS Homo sapiens
28 PXMP2  
Proximity Label-MS Homo sapiens
29 SLC2A4  
Affinity Capture-MS Homo sapiens
30 KCNK1  
Affinity Capture-MS Homo sapiens
31 CD70 970
Affinity Capture-MS Homo sapiens
32 PANX1 24145
Proximity Label-MS Homo sapiens
33 EMD 2010
Proximity Label-MS Homo sapiens
34 P2RY8  
Affinity Capture-MS Homo sapiens
35 GPR35  
Affinity Capture-MS Homo sapiens
36 S1PR1 1901
Affinity Capture-MS Homo sapiens
37 GPR182  
Affinity Capture-MS Homo sapiens
38 BRICD5  
Affinity Capture-MS Homo sapiens
39 RPN1 6184
Proximity Label-MS Homo sapiens
40 ELOVL5 60481
Proximity Label-MS Homo sapiens
41 GPR173  
Affinity Capture-MS Homo sapiens
42 SEC61B 10952
Proximity Label-MS Homo sapiens
43 CDIPT 10423
Affinity Capture-MS Homo sapiens
44 REEP5 7905
Proximity Label-MS Homo sapiens
45 BCAP31 10134
Proximity Label-MS Homo sapiens
46 VIPR2  
Affinity Capture-MS Homo sapiens
47 APLNR  
Affinity Capture-MS Homo sapiens
48 RPN2 6185
Proximity Label-MS Homo sapiens
49 HSD17B7 51478
Two-hybrid Homo sapiens
50 LRRC59 55379
Proximity Label-MS Homo sapiens
51 LMNB1 4001
Proximity Label-MS Homo sapiens
52 ZDHHC12  
Affinity Capture-MS Homo sapiens
53 LRRC25  
Affinity Capture-MS Homo sapiens
54 LAMP1 3916
Proximity Label-MS Homo sapiens
55 CMKLR1 1240
Affinity Capture-MS Homo sapiens
56 ERGIC3 51614
Affinity Capture-MS Homo sapiens
57 MGARP  
Affinity Capture-MS Homo sapiens
58 HSD3B7 80270
Proximity Label-MS Homo sapiens
59 STIM1 6786
Proximity Label-MS Homo sapiens
60 KLRD1  
Affinity Capture-MS Homo sapiens
61 KLRG2 346689
Affinity Capture-MS Homo sapiens
62 AQP3  
Affinity Capture-MS Homo sapiens
63 LPAR1 1902
Affinity Capture-MS Homo sapiens
64 NXF1 10482
Affinity Capture-RNA Homo sapiens
65 SYP  
Affinity Capture-MS Homo sapiens
66 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
67 RPA3 6119
Proximity Label-MS Homo sapiens
68 ATP2A1 487
Proximity Label-MS Homo sapiens
69 GPR45  
Affinity Capture-MS Homo sapiens
70 CMTM5  
Affinity Capture-MS Homo sapiens
71 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
72 CTDSP1 58190
Affinity Capture-MS Homo sapiens
73 TP53 7157
Affinity Capture-MS Homo sapiens
74 GP9 2815
Affinity Capture-MS Homo sapiens
75 OPALIN  
Affinity Capture-MS Homo sapiens
76 LDLRAD1  
Affinity Capture-MS Homo sapiens
77 MFSD4  
Affinity Capture-MS Homo sapiens
78 TMEM120B  
Affinity Capture-MS Homo sapiens
79 TSPAN15 23555
Affinity Capture-MS Homo sapiens
80 ERGIC1 57222
Proximity Label-MS Homo sapiens
81 AUP1 550
Affinity Capture-MS Homo sapiens
82 TSPAN8 7103
Affinity Capture-MS Homo sapiens
83 MOV10 4343
Affinity Capture-RNA Homo sapiens
84 SEC62 7095
Proximity Label-MS Homo sapiens
85 SPPL2B 56928
Affinity Capture-MS Homo sapiens
86 RAB7A 7879
Proximity Label-MS Homo sapiens
87 AGPAT6 137964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 VIPR1  
Affinity Capture-MS Homo sapiens
89 RAB5C 5878
Proximity Label-MS Homo sapiens
90 C9orf72  
Affinity Capture-MS Homo sapiens
91 CKAP4 10970
Proximity Label-MS Homo sapiens
92 SLC1A1 6505
Affinity Capture-MS Homo sapiens
93 CLEC4E  
Affinity Capture-MS Homo sapiens
94 FPR2  
Affinity Capture-MS Homo sapiens
95 DERL1 79139
Proximity Label-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here