Gene description for ATP12A
Gene name ATPase, H+/K+ transporting, nongastric, alpha polypeptide
Gene symbol ATP12A
Other names/aliases ATP1AL1
Species Homo sapiens
 Database cross references - ATP12A
ExoCarta ExoCarta_479
Entrez Gene 479
HGNC 13816
MIM 182360
UniProt P54707  
 ATP12A identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ATP12A
Molecular Function
    ATP binding GO:0005524 IEA
    sodium:potassium-exchanging ATPase activity GO:0005391 IEA
    metal ion binding GO:0046872 IEA
    hydrogen:potassium-exchanging ATPase activity GO:0008900 IEA
Biological Process
    transmembrane transport GO:0055085 TAS
    potassium ion transport GO:0006813 IEA
    regulation of pH GO:0006885 IEA
    sodium ion transmembrane transport GO:0035725 IEA
    potassium ion homeostasis GO:0055075 IEA
    hydrogen ion transmembrane transport GO:1902600 IEA
    metabolic process GO:0008152 IEA
    ion transmembrane transport GO:0034220 TAS
Subcellular Localization
    basolateral plasma membrane GO:0016323 IEA
    hydrogen:potassium-exchanging ATPase complex GO:0005889 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified ATP12A in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ATP12A
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ATP12A is involved
PathwayEvidenceSource
Ion transport by P-type ATPases TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here