Gene description for NAA30
Gene name N(alpha)-acetyltransferase 30, NatC catalytic subunit
Gene symbol NAA30
Other names/aliases C14orf35
MAK3
Mak3p
NAT12
NAT12P
Species Homo sapiens
 Database cross references - NAA30
ExoCarta ExoCarta_122830
Vesiclepedia VP_122830
Entrez Gene 122830
HGNC 19844
UniProt Q147X3  
 NAA30 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NAA30
Molecular Function
    peptide alpha-N-acetyltransferase activity GO:0004596 IBA
    peptide alpha-N-acetyltransferase activity GO:0004596 IDA
    protein binding GO:0005515 IPI
    peptide-methionine-alpha-N-acetyltransferase activity GO:0120518 IEA
Biological Process
    protein stabilization GO:0050821 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 IDA
    NatC complex GO:0031417 IBA
    NatC complex GO:0031417 IDA
    NatC complex GO:0031417 IPI
 Experiment description of studies that identified NAA30 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NAA30
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DUSP19  
Affinity Capture-MS Homo sapiens
2 NAA38  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CCDC28B  
Affinity Capture-MS Homo sapiens
4 ATP1A3 478
Affinity Capture-MS Homo sapiens
5 RTKN 6242
Affinity Capture-MS Homo sapiens
6 CD2BP2 10421
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 BTF3 689
Affinity Capture-MS Homo sapiens
8 ACTA2 59
Affinity Capture-MS Homo sapiens
9 RLIM 51132
Affinity Capture-MS Homo sapiens
10 EPB41L5 57669
Affinity Capture-MS Homo sapiens
11 TUBB8 347688
Affinity Capture-MS Homo sapiens
12 ATP12A 479
Affinity Capture-MS Homo sapiens
13 C1orf35  
Affinity Capture-MS Homo sapiens
14 TUBA1A 7846
Affinity Capture-MS Homo sapiens
15 WDTC1 23038
Affinity Capture-MS Homo sapiens
16 NKIRAS1  
Affinity Capture-MS Homo sapiens
17 NAA35 60560
Affinity Capture-MS Homo sapiens
18 MAP4K2  
Affinity Capture-MS Homo sapiens
19 ISYNA1 51477
Affinity Capture-MS Homo sapiens
20 HARS2 23438
Affinity Capture-MS Homo sapiens
21 NARS2  
Affinity Capture-MS Homo sapiens
22 PTAR1 375743
Synthetic Lethality Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here