Gene description for RTKN
Gene name rhotekin
Gene symbol RTKN
Other names/aliases -
Species Homo sapiens
 Database cross references - RTKN
ExoCarta ExoCarta_6242
Entrez Gene 6242
HGNC 10466
MIM 602288
UniProt Q9BST9  
 RTKN identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for RTKN
Molecular Function
    GTP-Rho binding GO:0017049 IDA
    GTPase inhibitor activity GO:0005095 IDA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
Biological Process
    Rho protein signal transduction GO:0007266 IDA
    small GTPase mediated signal transduction GO:0007264 TAS
    negative regulation of GTPase activity GO:0034260 IEA
    signal transduction GO:0007165 IDA
    apoptotic process GO:0006915 IEA
    septin cytoskeleton organization GO:0032185 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    cellular_component GO:0005575 ND
 Experiment description of studies that identified RTKN in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RTKN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RHOA 387
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
2 RHOC 389
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RTKN is involved
PathwayEvidenceSource
RHO GTPases Activate Rhotekin and Rhophilins TAS Reactome





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