Gene description for KIF1C
Gene name kinesin family member 1C
Gene symbol KIF1C
Other names/aliases LTXS1
SATX2
SAX2
SPAX2
SPG58
Species Homo sapiens
 Database cross references - KIF1C
ExoCarta ExoCarta_10749
Vesiclepedia VP_10749
Entrez Gene 10749
HGNC 6317
MIM 603060
UniProt O43896  
 KIF1C identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for KIF1C
Molecular Function
    RNA binding GO:0003723 HDA
    cytoskeletal motor activity GO:0003774 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    plus-end-directed microtubule motor activity GO:0008574 IBA
    ATP hydrolysis activity GO:0016887 IBA
Biological Process
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 TAS
    vesicle-mediated transport GO:0016192 IBA
    anterograde neuronal dense core vesicle transport GO:1990048 ISS
    retrograde neuronal dense core vesicle transport GO:1990049 IBA
    retrograde neuronal dense core vesicle transport GO:1990049 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    endoplasmic reticulum GO:0005783 TAS
    Golgi apparatus GO:0005794 TAS
    kinesin complex GO:0005871 IBA
    microtubule GO:0005874 IBA
    axon GO:0030424 IBA
    dendrite GO:0030425 IBA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified KIF1C in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
7
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
8
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
9
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for KIF1C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 POLR2G 5436
Affinity Capture-MS Homo sapiens
3 HDAC4  
Affinity Capture-MS Homo sapiens
4 HOOK1  
Proximity Label-MS Homo sapiens
5 GIGYF1  
Affinity Capture-MS Homo sapiens
6 ZNF346  
Affinity Capture-MS Homo sapiens
7 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
8 THOC2 57187
Affinity Capture-MS Homo sapiens
9 CDC25B 994
Affinity Capture-MS Homo sapiens
10 SRGAP2 23380
Affinity Capture-MS Homo sapiens
11 CALM1 801
Affinity Capture-MS Homo sapiens
12 CAMSAP2  
Affinity Capture-MS Homo sapiens
13 CBY1  
Affinity Capture-MS Homo sapiens
14 MYO1A 4640
Affinity Capture-MS Homo sapiens
15 UBE2N 7334
Affinity Capture-MS Homo sapiens
16 EIF4E2  
Affinity Capture-MS Homo sapiens
17 ILF2 3608
Affinity Capture-MS Homo sapiens
18 KIF13B 23303
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 GSPT2 23708
Affinity Capture-MS Homo sapiens
20 ZBTB21  
Affinity Capture-MS Homo sapiens
21 ACTR3 10096
Proximity Label-MS Homo sapiens
22 RASAL2 9462
Affinity Capture-MS Homo sapiens
23 ZNF638 27332
Affinity Capture-MS Homo sapiens
24 FBXW7  
Affinity Capture-MS Homo sapiens
25 CALM3 808
Affinity Capture-MS Homo sapiens
26 YWHAG 7532
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
27 TIAM1  
Affinity Capture-MS Homo sapiens
28 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 FAM110B  
Affinity Capture-MS Homo sapiens
30 LRFN1  
Affinity Capture-MS Homo sapiens
31 KSR1  
Affinity Capture-MS Homo sapiens
32 THOC1 9984
Affinity Capture-MS Homo sapiens
33 KIAA1804  
Affinity Capture-MS Homo sapiens
34 HSPA8 3312
Affinity Capture-MS Homo sapiens
35 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 AGAP1  
Affinity Capture-MS Homo sapiens
37 EPB41L3 23136
Affinity Capture-MS Homo sapiens
38 ANXA7 310
Affinity Capture-MS Homo sapiens
39 CDK16 5127
Affinity Capture-MS Homo sapiens
40 ANKRD34A  
Affinity Capture-MS Homo sapiens
41 USP21  
Affinity Capture-MS Homo sapiens
42 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
43 TESK2  
Affinity Capture-MS Homo sapiens
44 STAU1 6780
Affinity Capture-MS Homo sapiens
45 IFI27L1  
Affinity Capture-MS Homo sapiens
46 PTPN21 11099
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
47 RAN 5901
Affinity Capture-MS Homo sapiens
48 INTS7  
Affinity Capture-MS Homo sapiens
49 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
50 UTY  
Affinity Capture-MS Homo sapiens
51 LYST 1130
Affinity Capture-MS Homo sapiens
52 AGPS 8540
Proximity Label-MS Homo sapiens
53 KCTD3  
Affinity Capture-MS Homo sapiens
54 LIMA1 51474
Affinity Capture-MS Homo sapiens
55 LAMC3  
Two-hybrid Homo sapiens
56 KIF1B 23095
Affinity Capture-MS Homo sapiens
57 S100P 6286
Affinity Capture-MS Homo sapiens
58 PRPF4B 8899
Affinity Capture-MS Homo sapiens
59 DCLK1 9201
Affinity Capture-MS Homo sapiens
60 DES 1674
Affinity Capture-MS Homo sapiens
61 PSENEN  
Affinity Capture-MS Homo sapiens
62 INPP5E 56623
Affinity Capture-MS Homo sapiens
63 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
64 THOC5 8563
Affinity Capture-MS Homo sapiens
65 NADK 65220
Affinity Capture-MS Homo sapiens
66 ZC3H11A  
Affinity Capture-MS Homo sapiens
67 SYDE1 85360
Affinity Capture-MS Homo sapiens
68 HOOK3 84376
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
69 MAPRE3  
Proximity Label-MS Homo sapiens
70 CCDC9  
Affinity Capture-MS Homo sapiens
71 KIF1BP 26128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 INO80B 83444
Affinity Capture-MS Homo sapiens
73 CGN  
Affinity Capture-MS Homo sapiens
74 Junb  
Affinity Capture-MS Mus musculus
75 KRT19 3880
Proximity Label-MS Homo sapiens
76 Ccdc9  
Affinity Capture-MS Mus musculus
77 PAIP1 10605
Affinity Capture-MS Homo sapiens
78 ROBO2 6092
Affinity Capture-MS Homo sapiens
79 DENND4C 55667
Affinity Capture-MS Homo sapiens
80 KIF1A 547
Affinity Capture-MS Homo sapiens
81 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
82 GIGYF2 26058
Affinity Capture-MS Homo sapiens
83 MAPKAP1 79109
Affinity Capture-MS Homo sapiens
84 FAM53C 51307
Affinity Capture-MS Homo sapiens
85 TP53 7157
Affinity Capture-MS Homo sapiens
86 RTKN 6242
Affinity Capture-MS Homo sapiens
87 MKNK1  
Affinity Capture-MS Homo sapiens
88 DENND1A 57706
Affinity Capture-MS Homo sapiens
89 PPM1H  
Affinity Capture-MS Homo sapiens
90 SRSF12  
Affinity Capture-MS Homo sapiens
91 Ccdc64  
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
92 CDC25C  
Affinity Capture-MS Homo sapiens
93 DDX58 23586
Affinity Capture-RNA Homo sapiens
94 MMGT1 93380
Affinity Capture-MS Homo sapiens
95 PRDM5  
Affinity Capture-MS Homo sapiens
96 NBEAL1 65065
Affinity Capture-MS Homo sapiens
97 C20orf197  
Affinity Capture-MS Homo sapiens
98 NF1 4763
Affinity Capture-MS Homo sapiens
99 SRSF7 6432
Affinity Capture-MS Homo sapiens
100 PINK1  
Affinity Capture-MS Homo sapiens
101 CUL7 9820
Affinity Capture-MS Homo sapiens
102 MAST3  
Affinity Capture-MS Homo sapiens
103 Kif1c  
Affinity Capture-MS Mus musculus
104 MAGI1  
Affinity Capture-MS Homo sapiens
105 CASC3  
Affinity Capture-MS Homo sapiens
106 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
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