Experiment description of studies that identified MYO1A in exosomes

1 Experiment ID 286 ISEV standards ✔ CEM EV Biophysical techniques ✔ Alix|TSG101 EV Cytosolic markers ✔ CD63|CD81|EpCAM EV Membrane markers ✘ EV Negative markers ✔ DLS EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ. Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 Isolation/purification methods Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

2 Experiment ID 224 ISEV standards ✔ EM|AFM EV Biophysical techniques ✔ Alix|TSG101 EV Cytosolic markers ✔ CD63|CD81 EV Membrane markers ✔ GOLGA2 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

Protein-protein interactions for MYO1A

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CNN1 1264 Reconstituted Complex Homo sapiens 2 DISC1 27185 Two-hybrid Homo sapiens View the network

Pathways in which MYO1A is involved

No pathways found