Gene description for ROBO2
Gene name roundabout, axon guidance receptor, homolog 2 (Drosophila)
Gene symbol ROBO2
Other names/aliases SAX3
Species Homo sapiens
 Database cross references - ROBO2
ExoCarta ExoCarta_6092
Vesiclepedia VP_6092
Entrez Gene 6092
HGNC 10250
MIM 602431
UniProt Q9HCK4  
 ROBO2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for ROBO2
Molecular Function
    protein binding GO:0005515 IPI
    axon guidance receptor activity GO:0008046 ISS
    axon guidance receptor activity GO:0008046 NAS
    identical protein binding GO:0042802 IDA
Biological Process
    metanephros development GO:0001656 ISS
    ureteric bud development GO:0001657 IMP
    heart induction GO:0003129 ISS
    outflow tract septum morphogenesis GO:0003148 ISS
    aortic valve morphogenesis GO:0003180 ISS
    pulmonary valve morphogenesis GO:0003184 ISS
    endocardial cushion formation GO:0003272 ISS
    chemotaxis GO:0006935 IEA
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IDA
    axon guidance GO:0007411 ISS
    central nervous system development GO:0007417 NAS
    brain development GO:0007420 IEP
    axon midline choice point recognition GO:0016199 ISS
    olfactory bulb interneuron development GO:0021891 ISS
    retinal ganglion cell axon guidance GO:0031290 ISS
    cellular response to hormone stimulus GO:0032870 IEP
    aorta development GO:0035904 ISS
    positive regulation of Notch signaling pathway GO:0045747 ISS
    positive regulation of axonogenesis GO:0050772 IDA
    negative regulation of negative chemotaxis GO:0050925 IDA
    negative regulation of synapse assembly GO:0051964 ISS
    ventricular septum morphogenesis GO:0060412 ISS
    apoptotic process involved in luteolysis GO:0061364 IEP
    cell-cell adhesion GO:0098609 IBA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    axolemma GO:0030673 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ROBO2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ROBO2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPS6KA5 9252
Two-hybrid Homo sapiens
2 KIF2A 3796
Affinity Capture-MS Homo sapiens
3 HLA-DPB1 3115
Two-hybrid Homo sapiens
4 TRIM13  
Two-hybrid Homo sapiens
5 UBQLN4 56893
Two-hybrid Homo sapiens
6 NR4A1  
Two-hybrid Homo sapiens
7 VAPA 9218
Affinity Capture-MS Homo sapiens
8 GJA1 2697
Proximity Label-MS Homo sapiens
9 TMEM248 55069
Affinity Capture-MS Homo sapiens
10 BNIP3L  
Two-hybrid Homo sapiens
11 CAV1 857
Proximity Label-MS Homo sapiens
12 MYC  
Affinity Capture-MS Homo sapiens
13 Tmem109  
Affinity Capture-MS Mus musculus
14 LYN 4067
Proximity Label-MS Homo sapiens
15 GPR152  
Two-hybrid Homo sapiens
16 CENPU  
Two-hybrid Homo sapiens
17 Cep72  
Affinity Capture-MS Mus musculus
18 B3GAT1  
Proximity Label-MS Homo sapiens
19 TMEM237 65062
Two-hybrid Homo sapiens
20 PARD6B 84612
Affinity Capture-MS Homo sapiens
21 METTL3  
Affinity Capture-MS Homo sapiens
22 RDH11 51109
Two-hybrid Homo sapiens
23 EPHA2 1969
Proximity Label-MS Homo sapiens
24 KRAS 3845
Proximity Label-MS Homo sapiens
25 RHOB 388
Proximity Label-MS Homo sapiens
26 KIF1C 10749
Affinity Capture-MS Homo sapiens
27 NR1H2  
Two-hybrid Homo sapiens
28 OCLN 100506658
Proximity Label-MS Homo sapiens
29 CPE 1363
Two-hybrid Homo sapiens
View the network image/svg+xml



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