Gene description for CDK16
Gene name cyclin-dependent kinase 16
Gene symbol CDK16
Other names/aliases PCTAIRE
PCTAIRE1
PCTGAIRE
PCTK1
Species Homo sapiens
 Database cross references - CDK16
ExoCarta ExoCarta_5127
Vesiclepedia VP_5127
Entrez Gene 5127
HGNC 8749
MIM 311550
UniProt Q00536  
 CDK16 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
 Gene ontology annotations for CDK16
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IDA
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein serine kinase activity GO:0106310 IEA
Biological Process
    protein phosphorylation GO:0006468 TAS
    exocytosis GO:0006887 IBA
    exocytosis GO:0006887 ISS
    spermatogenesis GO:0007283 ISS
    spermatogenesis GO:0007283 NAS
    positive regulation of autophagy GO:0010508 IDA
    growth hormone secretion GO:0030252 ISS
    neuron projection development GO:0031175 IBA
    neuron projection development GO:0031175 ISS
    regulation of insulin secretion involved in cellular response to glucose stimulus GO:0061178 IMP
    regulation of cell cycle phase transition GO:1901987 IBA
Subcellular Localization
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 IPI
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    synaptic vesicle GO:0008021 IBA
    synaptic vesicle GO:0008021 ISS
    cytoplasmic side of plasma membrane GO:0009898 IDA
    microtubule cytoskeleton GO:0015630 IDA
    neuron projection GO:0043005 IEA
 Experiment description of studies that identified CDK16 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
11
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for CDK16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARHGAP21 57584
Affinity Capture-MS Homo sapiens
2 GTF2F1 2962
Affinity Capture-MS Homo sapiens
3 HDAC4  
Affinity Capture-MS Homo sapiens
4 Cdk1 12534
Affinity Capture-MS Mus musculus
5 KSR1  
Affinity Capture-MS Homo sapiens
6 MARCKS 4082
Proximity Label-MS Homo sapiens
7 LAMP3  
Proximity Label-MS Homo sapiens
8 PKM 5315
Affinity Capture-MS Homo sapiens
9 SH3BP4 23677
Affinity Capture-MS Homo sapiens
10 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 BRCA1 672
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CDC25B 994
Affinity Capture-MS Homo sapiens
13 SRGAP2 23380
Affinity Capture-MS Homo sapiens
14 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
15 CAMSAP2  
Affinity Capture-MS Homo sapiens
16 CBY1  
Affinity Capture-MS Homo sapiens
17 EIF4E2  
Affinity Capture-MS Homo sapiens
18 APP 351
Reconstituted Complex Homo sapiens
19 KIF13B 23303
Affinity Capture-MS Homo sapiens
20 MLLT4 4301
Affinity Capture-MS Homo sapiens
21 ZBTB21  
Affinity Capture-MS Homo sapiens
22 RASAL2 9462
Affinity Capture-MS Homo sapiens
23 ZNF638 27332
Affinity Capture-MS Homo sapiens
24 RAB3IP  
Affinity Capture-MS Homo sapiens
25 YWHAG 7532
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
26 C11orf52 91894
Proximity Label-MS Homo sapiens
27 TIAM1  
Affinity Capture-MS Homo sapiens
28 PHLDB2 90102
Affinity Capture-MS Homo sapiens
29 YWHAH 7533
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
30 ZBTB14  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 LRFN1  
Affinity Capture-MS Homo sapiens
32 RAB35 11021
Proximity Label-MS Homo sapiens
33 CSNK2B 1460
Affinity Capture-MS Homo sapiens
34 KIAA1804  
Affinity Capture-MS Homo sapiens
35 LAMTOR1 55004
Proximity Label-MS Homo sapiens
36 MELK  
Affinity Capture-MS Homo sapiens
37 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 AGAP1  
Affinity Capture-MS Homo sapiens
39 ANKRD34A  
Affinity Capture-MS Homo sapiens
40 USP21  
Affinity Capture-MS Homo sapiens
41 CDK5 1020
Biochemical Activity Homo sapiens
42 FAM110A  
Affinity Capture-MS Homo sapiens
43 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
44 TESK2  
Affinity Capture-MS Homo sapiens
45 LYN 4067
Proximity Label-MS Homo sapiens
46 CDK5R1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
47 YWHAQ 10971
Two-hybrid Homo sapiens
48 SPDYE4  
Affinity Capture-MS Homo sapiens
49 FAM110B  
Affinity Capture-MS Homo sapiens
50 KCTD3  
Affinity Capture-MS Homo sapiens
51 CCNY 219771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 CDK17 5128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 LIMA1 51474
Affinity Capture-MS Homo sapiens
54 DCLK1 9201
Affinity Capture-MS Homo sapiens
55 XPO1 7514
Affinity Capture-MS Homo sapiens
56 RC3H2  
Affinity Capture-MS Homo sapiens
57 CCNYL1 151195
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 LAMP1 3916
Proximity Label-MS Homo sapiens
59 SIRPA 140885
Reconstituted Complex Homo sapiens
60 CUL3 8452
Affinity Capture-MS Homo sapiens
61 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
62 ISG20L2 81875
Affinity Capture-MS Homo sapiens
63 ATRIP  
Two-hybrid Homo sapiens
64 ARF6 382
Proximity Label-MS Homo sapiens
65 NADK 65220
Affinity Capture-MS Homo sapiens
66 SNX27 81609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 SYDE1 85360
Affinity Capture-MS Homo sapiens
68 PLEKHA5 54477
Affinity Capture-MS Homo sapiens
69 CYP2C18  
Affinity Capture-MS Homo sapiens
70 CDK2 1017
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 MYC  
Synthetic Lethality Homo sapiens
72 RPA3 6119
Proximity Label-MS Homo sapiens
73 MCMBP 79892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 GIGYF1  
Affinity Capture-MS Homo sapiens
75 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 CGN  
Affinity Capture-MS Homo sapiens
77 Mllt1  
Affinity Capture-MS Mus musculus
78 RHOB 388
Proximity Label-MS Homo sapiens
79 CDKN1B 1027
Affinity Capture-MS Homo sapiens
80 PPP2CB 5516
Affinity Capture-MS Homo sapiens
81 TBC1D25  
Affinity Capture-MS Homo sapiens
82 DENND4C 55667
Affinity Capture-MS Homo sapiens
83 DEPDC1B 55789
Affinity Capture-MS Homo sapiens
84 NUP54 53371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
86 ICK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 MAPKAP1 79109
Affinity Capture-MS Homo sapiens
88 KIF14 9928
Affinity Capture-MS Homo sapiens
89 FAM53C 51307
Affinity Capture-MS Homo sapiens
90 CDK8 1024
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 RTKN 6242
Affinity Capture-MS Homo sapiens
92 DENND1A 57706
Affinity Capture-MS Homo sapiens
93 PPM1H  
Affinity Capture-MS Homo sapiens
94 SRSF12  
Affinity Capture-MS Homo sapiens
95 CDK18 5129
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 S100A10 6281
Two-hybrid Homo sapiens
97 INPPL1 3636
Affinity Capture-MS Homo sapiens
98 EDC3 80153
Affinity Capture-MS Homo sapiens
99 GAB2 9846
Affinity Capture-MS Homo sapiens
100 APPBP2  
Two-hybrid Homo sapiens
101 NF1 4763
Affinity Capture-MS Homo sapiens
102 RAB2A 5862
Proximity Label-MS Homo sapiens
103 RAB11A 8766
Proximity Label-MS Homo sapiens
104 PLEKHA7 144100
Affinity Capture-MS Homo sapiens
105 EPHA2 1969
Proximity Label-MS Homo sapiens
106 SERBP1 26135
Affinity Capture-MS Homo sapiens
107 KRAS 3845
Proximity Label-MS Homo sapiens
108 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
109 KIF1C 10749
Affinity Capture-MS Homo sapiens
110 MAST3  
Affinity Capture-MS Homo sapiens
111 PPP2R2A 5520
Affinity Capture-MS Homo sapiens
112 MAGI1  
Affinity Capture-MS Homo sapiens
113 PPP2R2D 55844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
115 CCND3  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CDK16 is involved
No pathways found





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