Gene description for Cdk1
Gene name cyclin-dependent kinase 1
Gene symbol Cdk1
Other names/aliases Cdc2
Cdc2a
p34
Species Mus musculus
 Database cross references - Cdk1
ExoCarta ExoCarta_12534
Vesiclepedia VP_12534
Entrez Gene 12534
UniProt P11440  
 Cdk1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cdk1
Molecular Function
    histone kinase activity GO:0035173 ISO
    nucleotide binding GO:0000166 IEA
    Hsp70 protein binding GO:0030544 IPI
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    kinase activity GO:0016301 IDA
    protein binding GO:0005515 IPI
    cyclin binding GO:0030332 ISO
    transferase activity GO:0016740 IEA
    protein serine/threonine kinase activity GO:0004674 ISO
    cyclin-dependent protein serine/threonine kinase activity GO:0004693 ISO
    RNA polymerase II carboxy-terminal domain kinase activity GO:0008353 ISO
    protein kinase activity GO:0004672 ISO
    ATP binding GO:0005524 IEA
Biological Process
    positive regulation of mitotic cell cycle GO:0045931 ISO
    peptidyl-serine phosphorylation GO:0018105 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    protein phosphorylation GO:0006468 IDA
    apoptotic process GO:0006915 IEA
    positive regulation of gene expression GO:0010628 ISO
    positive regulation of protein import into nucleus, translocation GO:0033160 ISO
    positive regulation of DNA replication GO:0045740 ISO
    peptidyl-threonine phosphorylation GO:0018107 ISO
    response to organonitrogen compound GO:0010243 ISO
    mitotic nuclear division GO:0007067 IEA
    cell cycle GO:0007049 IEA
    response to ethanol GO:0045471 ISO
    cell aging GO:0007569 ISO
    organ regeneration GO:0031100 ISO
    positive regulation of cardiac muscle cell proliferation GO:0060045 ISO
    histone phosphorylation GO:0016572 ISO
    cell division GO:0051301 IEA
    protein localization to kinetochore GO:0034501 ISO
    protein complex assembly GO:0006461 ISO
    mitotic G2 DNA damage checkpoint GO:0007095 IDA
    phosphorylation GO:0016310 IEA
    chromosome condensation GO:0030261 ISO
Subcellular Localization
    centrosome GO:0005813 ISO
    nucleus GO:0005634 ISO
    spindle microtubule GO:0005876 ISO
    cytoskeleton GO:0005856 IEA
    midbody GO:0030496 ISO
    mitotic spindle GO:0072686 ISO
    membrane GO:0016020 ISO
    mitochondrion GO:0005739 IEA
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Cdk1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cdk1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Cdk1 is involved
PathwayEvidenceSource
Activation of NIMA Kinases NEK9, NEK6, NEK7 IEA Reactome
Anchoring of the basal body to the plasma membrane IEA Reactome
APC/C:Cdc20 mediated degradation of Cyclin B IEA Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A IEA Reactome
Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex IEA Reactome
Condensation of Prometaphase Chromosomes IEA Reactome
Condensation of Prophase Chromosomes IEA Reactome
Cyclin A/B1 associated events during G2/M transition IEA Reactome
Cyclin B2 mediated events IEA Reactome
Depolymerisation of the Nuclear Lamina IEA Reactome
E2F mediated regulation of DNA replication IEA Reactome
E2F-enabled inhibition of pre-replication complex formation IEA Reactome
ERK1 activation IEA Reactome
ERK1 activation IEA Reactome
G0 and Early G1 IEA Reactome
G1/S-Specific Transcription IEA Reactome
G2/M DNA replication checkpoint IEA Reactome
Golgi Cisternae Pericentriolar Stack Reorganization IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
MASTL Facilitates Mitotic Progression IEA Reactome
Nuclear Pore Complex (NPC) Disassembly IEA Reactome
Phosphorylation of Emi1 IEA Reactome
Phosphorylation of the APC/C IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Regulation of APC/C activators between G1/S and early anaphase IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome





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