Gene description for AP3S1
Gene name adaptor-related protein complex 3, sigma 1 subunit
Gene symbol AP3S1
Other names/aliases CLAPS3
Sigma3A
Species Homo sapiens
 Database cross references - AP3S1
ExoCarta ExoCarta_1176
Vesiclepedia VP_1176
Entrez Gene 1176
HGNC 2013
MIM 601507
UniProt Q92572  
 AP3S1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for AP3S1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    intracellular protein transport GO:0006886 IEA
    Golgi to vacuole transport GO:0006896 IEA
    anterograde axonal transport GO:0008089 ISS
    insulin receptor signaling pathway GO:0008286 TAS
    synaptic vesicle coating GO:0016183 NAS
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 NAS
    clathrin-coated vesicle cargo loading, AP-3-mediated GO:0035654 NAS
    synaptic vesicle recycling GO:0036465 NAS
    intracellular transport GO:0046907 NAS
    anterograde synaptic vesicle transport GO:0048490 ISS
    platelet dense granule organization GO:0060155 NAS
    melanosome assembly GO:1903232 NAS
Subcellular Localization
    early endosome GO:0005769 NAS
    Golgi apparatus GO:0005794 IEA
    AP-type membrane coat adaptor complex GO:0030119 TAS
    AP-3 adaptor complex GO:0030123 IDA
    AP-3 adaptor complex GO:0030123 NAS
    transport vesicle GO:0030133 TAS
    cytoplasmic vesicle membrane GO:0030659 IEA
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
    presynapse GO:0098793 IEA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified AP3S1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for AP3S1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 CSTL1  
Affinity Capture-MS Homo sapiens
3 Cdk1 12534
Affinity Capture-MS Mus musculus
4 EEF1G 1937
Affinity Capture-MS Homo sapiens
5 KIAA1107  
Affinity Capture-MS Homo sapiens
6 RNF167 26001
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 KLK5 25818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ATG9A 79065
Proximity Label-MS Homo sapiens
9 SRPRB 58477
Affinity Capture-MS Homo sapiens
10 RPA2 6118
Proximity Label-MS Homo sapiens
11 ARF3 377
Proximity Label-MS Homo sapiens
12 LAMP2 3920
Proximity Label-MS Homo sapiens
13 SPG7  
Affinity Capture-MS Homo sapiens
14 MUC20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 IGSF6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CHODL 140578
Affinity Capture-MS Homo sapiens
17 AGO3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ZNF460  
Affinity Capture-MS Homo sapiens
19 DUSP2  
Affinity Capture-MS Homo sapiens
20 DUSP5  
Affinity Capture-MS Homo sapiens
21 PMEL 6490
Affinity Capture-MS Homo sapiens
22 FBXO11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TACSTD2 4070
Affinity Capture-MS Homo sapiens
24 ATG16L1 55054
Affinity Capture-MS Homo sapiens
25 CD14 929
Affinity Capture-MS Homo sapiens
26 ZWINT  
Affinity Capture-MS Homo sapiens
27 LAMTOR1 55004
Proximity Label-MS Homo sapiens
28 SMC4 10051
Co-fractionation Homo sapiens
29 EPB41L3 23136
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 VSIG1  
Affinity Capture-MS Homo sapiens
31 AP3M1 26985
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 DLD 1738
Affinity Capture-MS Homo sapiens
33 RUFY1 80230
Reconstituted Complex Homo sapiens
34 AGK 55750
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 STAU1 6780
Affinity Capture-MS Homo sapiens
36 DNAJC5 80331
Affinity Capture-MS Homo sapiens
37 VSIG4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 DHRS2 10202
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 DTNBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SP110  
Affinity Capture-MS Homo sapiens
41 RPA4  
Proximity Label-MS Homo sapiens
42 TMEM63A 9725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 AP3B1 8546
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 ZYG11B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 DUSP23 54935
Affinity Capture-MS Homo sapiens
46 DUSP1  
Affinity Capture-MS Homo sapiens
47 CDC73  
Co-fractionation Homo sapiens
48 ICAM1 3383
Affinity Capture-MS Homo sapiens
49 XPO1 7514
Affinity Capture-MS Homo sapiens
50 ECT2 1894
Affinity Capture-MS Homo sapiens
51 KLK11 11012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 PCDHB15  
Affinity Capture-MS Homo sapiens
53 ATP5F1 515
Affinity Capture-MS Homo sapiens
54 WDR55  
Affinity Capture-MS Homo sapiens
55 EGFR 1956
Affinity Capture-MS Homo sapiens
56 DUSP6 1848
Affinity Capture-MS Homo sapiens
57 BLK 640
Affinity Capture-MS Homo sapiens
58 Mast3  
Affinity Capture-MS Mus musculus
59 AP3M2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 EMC2 9694
Affinity Capture-MS Homo sapiens
61 FNDC3A 22862
Affinity Capture-MS Homo sapiens
62 TPCN2  
Affinity Capture-MS Homo sapiens
63 RNF13 11342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
64 DUSP19  
Affinity Capture-MS Homo sapiens
65 CLPP 8192
Proximity Label-MS Homo sapiens
66 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
67 TRIM13  
Affinity Capture-MS Homo sapiens
68 Tmed10 68581
Affinity Capture-MS Mus musculus
69 SIDT2 51092
Affinity Capture-MS Homo sapiens
70 PGRMC1 10857
Affinity Capture-MS Homo sapiens
71 ARF5 381
Proximity Label-MS Homo sapiens
72 CLPTM1 1209
Affinity Capture-MS Homo sapiens
73 PLCD3 113026
Affinity Capture-MS Homo sapiens
74 LCK 3932
Proximity Label-MS Homo sapiens
75 PLEKHJ1  
Affinity Capture-MS Homo sapiens
76 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
77 COX8A  
Proximity Label-MS Homo sapiens
78 RABL6 55684
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 ARF4 378
Proximity Label-MS Homo sapiens
80 IRS1 3667
Reconstituted Complex Homo sapiens
81 TRIM37  
Proximity Label-MS Homo sapiens
82 Kif4  
Affinity Capture-MS Mus musculus
83 SSSCA1 10534
Co-fractionation Homo sapiens
84 AP3B2 8120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 FLNC 2318
Co-fractionation Homo sapiens
86 DUSP4 1846
Affinity Capture-MS Homo sapiens
87 SERBP1 26135
Affinity Capture-MS Homo sapiens
88 IRGC  
Affinity Capture-MS Homo sapiens
89 AP3D1 8943
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 RPA3 6119
Proximity Label-MS Homo sapiens
91 KRT7 3855
Co-fractionation Homo sapiens
View the network image/svg+xml



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