Gene description for AGK
Gene name acylglycerol kinase
Gene symbol AGK
Other names/aliases CATC5
CTRCT38
MTDPS10
MULK
Species Homo sapiens
 Database cross references - AGK
ExoCarta ExoCarta_55750
Vesiclepedia VP_55750
Entrez Gene 55750
HGNC 21869
MIM 610345
UniProt Q53H12  
 AGK identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for AGK
Molecular Function
    ceramide kinase activity GO:0001729 IBA
    ATP-dependent diacylglycerol kinase activity GO:0004143 IBA
    ATP-dependent diacylglycerol kinase activity GO:0004143 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    D-erythro-sphingosine kinase activity GO:0017050 IBA
    acylglycerol kinase activity GO:0047620 IBA
    acylglycerol kinase activity GO:0047620 IDA
Biological Process
    protein insertion into mitochondrial inner membrane GO:0045039 IDA
    glycerolipid metabolic process GO:0046486 IEA
    sphingosine biosynthetic process GO:0046512 IBA
    ceramide biosynthetic process GO:0046513 IBA
    lipid phosphorylation GO:0046834 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 TAS
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial intermembrane space GO:0005758 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IBA
    mitochondrial membrane GO:0031966 IDA
    TIM22 mitochondrial import inner membrane insertion complex GO:0042721 IDA
    TIM22 mitochondrial import inner membrane insertion complex GO:0042721 IPI
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified AGK in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AGK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AP3S1 1176
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 INTS4  
Affinity Capture-MS Homo sapiens
4 HECTD1 25831
Affinity Capture-MS Homo sapiens
5 FAS 355
Affinity Capture-MS Homo sapiens
6 ZRANB1 54764
Affinity Capture-MS Homo sapiens
7 MECP2 4204
Affinity Capture-MS Homo sapiens
8 FGD5  
Affinity Capture-MS Homo sapiens
9 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
10 MYCN  
Affinity Capture-MS Homo sapiens
11 CBX1 10951
Affinity Capture-MS Homo sapiens
12 FARP2 9855
Affinity Capture-MS Homo sapiens
13 SLC25A12 8604
Proximity Label-MS Homo sapiens
14 UBE2H 7328
Affinity Capture-MS Homo sapiens
15 RAB29 8934
Affinity Capture-MS Homo sapiens
16 FBXL6  
Affinity Capture-MS Homo sapiens
17 LAMTOR1 55004
Proximity Label-MS Homo sapiens
18 ATP1A3 478
Affinity Capture-MS Homo sapiens
19 C9orf78 51759
Affinity Capture-MS Homo sapiens
20 LMNB1 4001
Affinity Capture-MS Homo sapiens
21 ICT1 3396
Affinity Capture-MS Homo sapiens
22 METTL13 51603
Affinity Capture-MS Homo sapiens
23 KLF16  
Affinity Capture-MS Homo sapiens
24 TIMM9  
Affinity Capture-MS Homo sapiens
25 XPO1 7514
Affinity Capture-MS Homo sapiens
26 UFL1 23376
Affinity Capture-MS Homo sapiens
27 RSPRY1 89970
Affinity Capture-MS Homo sapiens
28 RYK 6259
Affinity Capture-MS Homo sapiens
29 ATP12A 479
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TIMM10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CUL3 8452
Affinity Capture-MS Homo sapiens
32 NR3C1 2908
Proximity Label-MS Homo sapiens
33 GPR107 57720
Affinity Capture-MS Homo sapiens
34 PARK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 NDUFA4 4697
Affinity Capture-MS Homo sapiens
36 OTUB1 55611
Affinity Capture-MS Homo sapiens
37 KIAA0368 23392
Affinity Capture-MS Homo sapiens
38 RIPK4  
Affinity Capture-MS Homo sapiens
39 RAB18 22931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 PRDM1  
Affinity Capture-MS Homo sapiens
41 SFXN1 94081
Proximity Label-MS Homo sapiens
42 HCCS 3052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 AURKB 9212
Affinity Capture-MS Homo sapiens
44 KIAA1429 25962
Affinity Capture-MS Homo sapiens
45 WWP2 11060
Affinity Capture-MS Homo sapiens
46 PRKACA 5566
Affinity Capture-MS Homo sapiens
47 AGO4  
Affinity Capture-MS Homo sapiens
48 TTI1 9675
Affinity Capture-MS Homo sapiens
49 FBXO6 26270
Affinity Capture-MS Homo sapiens
50 COX11  
Affinity Capture-MS Homo sapiens
51 NTRK1 4914
Affinity Capture-MS Homo sapiens
52 COQ9  
Affinity Capture-MS Homo sapiens
53 C19orf52  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 SPRTN  
Affinity Capture-MS Homo sapiens
55 TIMM10B 26515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 PRSS8 5652
Affinity Capture-MS Homo sapiens
57 EP300 2033
Affinity Capture-MS Homo sapiens
58 PCSK9 255738
Affinity Capture-MS Homo sapiens
59 RNF2  
Affinity Capture-MS Homo sapiens
60 BMI1  
Affinity Capture-MS Homo sapiens
61 DDRGK1 65992
Affinity Capture-MS Homo sapiens
62 TUBA1A 7846
Affinity Capture-MS Homo sapiens
63 DYM  
Affinity Capture-MS Homo sapiens
64 ATP2C1 27032
Affinity Capture-MS Homo sapiens
65 C2CD2 25966
Affinity Capture-MS Homo sapiens
66 C9orf72  
Affinity Capture-MS Homo sapiens
67 NEU1 4758
Affinity Capture-MS Homo sapiens
68 TULP3 7289
Affinity Capture-MS Homo sapiens
69 TNFAIP2 7127
Affinity Capture-MS Homo sapiens
70 DERL1 79139
Proximity Label-MS Homo sapiens
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