Gene description for FARP2
Gene name FERM, RhoGEF and pleckstrin domain protein 2
Gene symbol FARP2
Other names/aliases FIR
FRG
PLEKHC3
Species Homo sapiens
 Database cross references - FARP2
ExoCarta ExoCarta_9855
Vesiclepedia VP_9855
Entrez Gene 9855
HGNC 16460
UniProt O94887  
 FARP2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for FARP2
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    cytoskeletal protein binding GO:0008092 IEA
Biological Process
    cell adhesion GO:0007155 ISS
    neuron remodeling GO:0016322 IDA
    Rac protein signal transduction GO:0016601 IDA
    hair cycle process GO:0022405 IEA
    actin cytoskeleton organization GO:0030036 ISS
    osteoclast differentiation GO:0030316 ISS
    regulation of integrin activation GO:0033623 ISS
    semaphorin-plexin signaling pathway GO:0071526 ISS
    podosome assembly GO:0071800 ISS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
 Experiment description of studies that identified FARP2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for FARP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TACR3  
Affinity Capture-MS Homo sapiens
2 SSR4 6748
Affinity Capture-MS Homo sapiens
3 PRICKLE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 PSD2  
Affinity Capture-MS Homo sapiens
5 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
6 MYCBP 26292
Affinity Capture-MS Homo sapiens
7 RPS27A 6233
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 RIOK1 83732
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CHN1  
Affinity Capture-MS Homo sapiens
10 C17orf85  
Affinity Capture-MS Homo sapiens
11 MAX  
Affinity Capture-MS Homo sapiens
12 NEURL4  
Affinity Capture-MS Homo sapiens
13 GAR1 54433
Affinity Capture-MS Homo sapiens
14 TP53 7157
Affinity Capture-MS Homo sapiens
15 GPR156  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 XPO1 7514
Affinity Capture-MS Homo sapiens
17 PIP 5304
Affinity Capture-MS Homo sapiens
18 MGA  
Affinity Capture-MS Homo sapiens
19 AGK 55750
Affinity Capture-MS Homo sapiens
20 BSG 682
Co-fractionation Homo sapiens
21 NUP93 9688
Affinity Capture-MS Homo sapiens
22 TRIM41 90933
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 MISP 126353
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 COPRS  
Affinity Capture-MS Homo sapiens
25 SRP9 6726
Affinity Capture-MS Homo sapiens
26 CD6 923
Affinity Capture-MS Homo sapiens
27 VAV1 7409
Affinity Capture-MS Homo sapiens
28 RPS19 6223
Affinity Capture-MS Homo sapiens
29 CLNS1A 1207
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PRKCI 5584
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TMEM263 90488
Affinity Capture-MS Homo sapiens
32 RBFOX2 23543
Affinity Capture-MS Homo sapiens
33 CDK6 1021
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
34 C11orf52 91894
Proximity Label-MS Homo sapiens
35 RIPK4  
Affinity Capture-MS Homo sapiens
36 PNKD  
Affinity Capture-MS Homo sapiens
37 LYN 4067
Proximity Label-MS Homo sapiens
38 UBE2G2  
Two-hybrid Homo sapiens
39 CHRM3 1131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 PRMT5 10419
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 KCNE3  
Affinity Capture-MS Homo sapiens
42 NOC2L 26155
Affinity Capture-MS Homo sapiens
43 RNPS1 10921
Affinity Capture-MS Homo sapiens
44 CDH1 999
Reconstituted Complex Homo sapiens
45 PSPC1 55269
Affinity Capture-MS Homo sapiens
46 CDK4 1019
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
47 ARRDC2  
Affinity Capture-MS Homo sapiens
48 WWC1  
Affinity Capture-MS Homo sapiens
49 EPHA2 1969
Proximity Label-MS Homo sapiens
50 ARRDC3 57561
Affinity Capture-MS Homo sapiens
51 CSNK1E 1454
Affinity Capture-MS Homo sapiens
52 NSRP1  
Affinity Capture-MS Homo sapiens
53 KRAS 3845
Proximity Label-MS Homo sapiens
54 CCDC96  
Affinity Capture-MS Homo sapiens
55 CCDC88A 55704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 PDZK1 5174
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
57 NOG 9241
Affinity Capture-MS Homo sapiens
58 MAGEB2 4113
Affinity Capture-MS Homo sapiens
59 DNAJB1 3337
Affinity Capture-MS Homo sapiens
60 C3orf49  
Affinity Capture-MS Homo sapiens
61 PTGER3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 STIP1 10963
Affinity Capture-MS Homo sapiens
63 HERC2 8924
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 TUBB4A 10382
Affinity Capture-MS Homo sapiens
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