Gene description for RIOK1
Gene name RIO kinase 1
Gene symbol RIOK1
Other names/aliases AD034
RRP10
bA288G3.1
Species Homo sapiens
 Database cross references - RIOK1
ExoCarta ExoCarta_83732
Vesiclepedia VP_83732
Entrez Gene 83732
HGNC 18656
UniProt Q9BRS2  
 RIOK1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RIOK1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    hydrolase activity GO:0016787 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    maturation of SSU-rRNA GO:0030490 IBA
    maturation of SSU-rRNA GO:0030490 IMP
    ribosomal small subunit biogenesis GO:0042274 IMP
    positive regulation of rRNA processing GO:2000234 IMP
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    preribosome, small subunit precursor GO:0030688 IBA
    preribosome, small subunit precursor GO:0030688 IDA
    methyltransferase complex GO:0034708 IMP
 Experiment description of studies that identified RIOK1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RIOK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRBN  
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 UBL4A 8266
Affinity Capture-MS Homo sapiens
5 COIL  
Proximity Label-MS Homo sapiens
6 RPS3A 6189
Co-crystal Structure Homo sapiens
7 RPS4X 6191
Co-crystal Structure Homo sapiens
8 RPS17 6218
Co-crystal Structure Homo sapiens
9 CIRBP 1153
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 COPRS  
Affinity Capture-MS Homo sapiens
11 RPS19 6223
Co-crystal Structure Homo sapiens
12 RPL15 6138
Affinity Capture-MS Homo sapiens
13 LSM4 25804
Affinity Capture-MS Homo sapiens
14 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
15 APP 351
Reconstituted Complex Homo sapiens
16 FAU 2197
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
17 KIAA1429 25962
Affinity Capture-MS Homo sapiens
18 RPS20 6224
Proximity Label-MS Homo sapiens
19 UBE2O 63893
Affinity Capture-MS Homo sapiens
20 TRMT112 51504
Co-crystal Structure Homo sapiens
21 RRP12 23223
Co-crystal Structure Homo sapiens
22 FBXW7  
Affinity Capture-MS Homo sapiens
23 RPS8 6202
Co-crystal Structure Homo sapiens
24 FBL 2091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
25 RPS9 6203
Co-crystal Structure Homo sapiens
26 TINAGL1 64129
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SMU1 55234
Affinity Capture-MS Homo sapiens
28 FAM207A  
Co-crystal Structure Homo sapiens
29 LACTB 114294
Affinity Capture-MS Homo sapiens
30 SNRPD3 6634
Affinity Capture-MS Homo sapiens
31 SERF2 10169
Affinity Capture-MS Homo sapiens
32 CSNK2B 1460
Affinity Capture-MS Homo sapiens
33 RPS11 6205
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
34 FAM177A1 283635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 DYNLT1 6993
Proximity Label-MS Homo sapiens
36 KIF14 9928
Affinity Capture-MS Homo sapiens
37 GAR1 54433
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 YWHAB 7529
Affinity Capture-MS Homo sapiens
39 EPB41L3 23136
Affinity Capture-MS Homo sapiens
40 ZHX1  
Affinity Capture-MS Homo sapiens
41 PRPF6 24148
Affinity Capture-MS Homo sapiens
42 DDX31  
Affinity Capture-MS Homo sapiens
43 PRPF8 10594
Affinity Capture-MS Homo sapiens
44 TOP3B 8940
Affinity Capture-MS Homo sapiens
45 RPS28 6234
Co-crystal Structure Homo sapiens
46 CLNS1A 1207
Affinity Capture-MS Homo sapiens
47 CFDP1  
Affinity Capture-MS Homo sapiens
48 PNO1 56902
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
49 SNRNP200 23020
Affinity Capture-MS Homo sapiens
50 AKR7L 246181
Affinity Capture-MS Homo sapiens
51 PMAIP1  
Affinity Capture-MS Homo sapiens
52 WDYHV1  
Affinity Capture-MS Homo sapiens
53 NTRK1 4914
Affinity Capture-MS Homo sapiens
54 IFI16 3428
Affinity Capture-MS Homo sapiens
55 NSRP1  
Affinity Capture-MS Homo sapiens
56 SNRPN 6638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 FBXL6  
Proximity Label-MS Homo sapiens
58 NOB1  
Affinity Capture-MS Homo sapiens
59 RPS6 6194
Co-crystal Structure Homo sapiens
60 MAGOH 4116
Affinity Capture-MS Homo sapiens
61 RPS15A 6210
Co-crystal Structure Homo sapiens
62 GAN 8139
Affinity Capture-MS Homo sapiens
63 LPPR1  
Affinity Capture-MS Homo sapiens
64 RPS2 6187
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
65 XPO1 7514
Affinity Capture-MS Homo sapiens
66 SNRPB 6628
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 WBSCR22  
Co-crystal Structure Homo sapiens
68 TBC1D10B 26000
Affinity Capture-MS Homo sapiens
69 PTEN 5728
Affinity Capture-MS Homo sapiens
70 DCAF13 25879
Affinity Capture-MS Homo sapiens
71 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
72 LRRC47 57470
Affinity Capture-MS Homo sapiens
73 FARP2 9855
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 FBXO6 26270
Affinity Capture-MS Homo sapiens
75 EFTUD2 9343
Affinity Capture-MS Homo sapiens
76 RPS27 6232
Co-crystal Structure Homo sapiens
77 PRMT5 10419
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
78 RBFOX1 54715
Affinity Capture-MS Homo sapiens
79 RPA3 6119
Proximity Label-MS Homo sapiens
80 RPS13 6207
Co-crystal Structure Homo sapiens
81 EMC2 9694
Affinity Capture-MS Homo sapiens
82 RPS14 6208
Co-crystal Structure Homo sapiens
83 RPS18 6222
Co-crystal Structure Homo sapiens
84 TSR1 55720
Co-crystal Structure Homo sapiens
85 RPS16 6217
Co-crystal Structure Homo sapiens
86 EIF1AD 84285
Affinity Capture-MS Homo sapiens
87 Prmt5 27374
Affinity Capture-MS Mus musculus
88 RPS27A 6233
Co-crystal Structure Homo sapiens
89 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
90 C1orf115  
Affinity Capture-MS Homo sapiens
91 RPS25 6230
Co-crystal Structure Homo sapiens
92 WDR77 79084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 CHTOP  
Affinity Capture-MS Homo sapiens
94 RPS21 6227
Co-crystal Structure Homo sapiens
95 Fbl 14113
Affinity Capture-MS Mus musculus
96 BYSL 705
Co-crystal Structure Homo sapiens
97 RPS12 6206
Co-crystal Structure Homo sapiens
98 SMURF1 57154
Affinity Capture-MS Homo sapiens
99 RPS15 6209
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
100 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
101 G3BP2 9908
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
102 EIF4A3 9775
Affinity Capture-MS Homo sapiens
103 RPS5 6193
Co-crystal Structure Homo sapiens
104 ESR1  
Affinity Capture-MS Homo sapiens
105 UFL1 23376
Affinity Capture-MS Homo sapiens
106 PPP1R14A 94274
Affinity Capture-MS Homo sapiens
107 RBFOX2 23543
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 RPS24 6229
Co-crystal Structure Homo sapiens
109 NCL 4691
Affinity Capture-MS Homo sapiens
110 RPS23 6228
Co-crystal Structure Homo sapiens
111 Kif4  
Affinity Capture-MS Mus musculus
112 RPS7 6201
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
113 SERBP1 26135
Proximity Label-MS Homo sapiens
114 LSM6 11157
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 SNW1 22938
Affinity Capture-MS Homo sapiens
116 GNB2L1 10399
Affinity Capture-MS Homo sapiens
117 RC3H1 149041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 PRMT1 3276
Affinity Capture-MS Homo sapiens
119 RPSA 3921
Co-crystal Structure Homo sapiens
120 EP300 2033
Affinity Capture-MS Homo sapiens
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