Gene description for Fbl
Gene name fibrillarin
Gene symbol Fbl
Other names/aliases AL022665
FIB
FLRN
RNU3IP1
Species Mus musculus
 Database cross references - Fbl
ExoCarta ExoCarta_14113
Vesiclepedia VP_14113
Entrez Gene 14113
UniProt P35550  
 Fbl identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Fbl
Molecular Function
    TFIID-class transcription factor complex binding GO:0001094 IEA
    TFIID-class transcription factor complex binding GO:0001094 ISO
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IPI
    rRNA methyltransferase activity GO:0008649 IBA
    ATPase binding GO:0051117 IEA
    ATPase binding GO:0051117 ISO
    U6 snRNA 2'-O-ribose methyltransferase activity GO:0180021 IEA
    histone H2AQ104 methyltransferase activity GO:1990259 IBA
    histone H2AQ104 methyltransferase activity GO:1990259 ISO
    histone H2AQ104 methyltransferase activity GO:1990259 ISS
Biological Process
    box C/D sno(s)RNA 3'-end processing GO:0000494 IBA
    chromatin remodeling GO:0006338 IEA
    sno(s)RNA metabolic process GO:0016074 IMP
    rRNA methylation GO:0031167 IBA
    rRNA methylation GO:0031167 ISO
    rRNA methylation GO:0031167 ISS
    ribosomal small subunit biogenesis GO:0042274 ISO
    ribosomal small subunit biogenesis GO:0042274 ISS
    snoRNA localization GO:0048254 IEA
    snoRNA localization GO:0048254 ISO
Subcellular Localization
    fibrillar center GO:0001650 IEA
    fibrillar center GO:0001650 ISO
    dense fibrillar component GO:0001651 ISO
    granular component GO:0001652 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    chromosome GO:0005694 ISO
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    nucleolus GO:0005730 ISO
    nucleolus GO:0005730 ISS
    Cajal body GO:0015030 IBA
    Cajal body GO:0015030 IDA
    Cajal body GO:0015030 ISO
    box C/D methylation guide snoRNP complex GO:0031428 IBA
    small-subunit processome GO:0032040 IBA
    small-subunit processome GO:0032040 ISO
    small-subunit processome GO:0032040 ISS
 Experiment description of studies that identified Fbl in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Fbl
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIOK1 83732
Affinity Capture-MS Homo sapiens
2 CLK1  
Two-hybrid Homo sapiens
3 NOP58 51602
Affinity Capture-MS Homo sapiens
4 MRPS2 51116
Affinity Capture-MS Homo sapiens
5 COIL  
Affinity Capture-MS Homo sapiens
6 CHAF1B  
Affinity Capture-MS Homo sapiens
7 DHCR24 1718
Affinity Capture-MS Homo sapiens
8 YTHDF1 54915
Two-hybrid Homo sapiens
9 JADE1  
Affinity Capture-MS Homo sapiens
10 ABCF1 23
Affinity Capture-MS Homo sapiens
11 Atxn1  
Affinity Capture-MS Mus musculus
12 NOP56 10528
Affinity Capture-MS Homo sapiens
13 COPS8 10920
Affinity Capture-MS Homo sapiens
14 SMNDC1  
Two-hybrid Homo sapiens
15 Tfe3  
Affinity Capture-MS Mus musculus
16 PINX1  
Affinity Capture-MS Homo sapiens
17 RRP9 9136
Affinity Capture-MS Homo sapiens
18 RPS27 6232
Affinity Capture-MS Homo sapiens
19 PRMT5 10419
Affinity Capture-MS Homo sapiens
20 BECN1 8678
Affinity Capture-MS Homo sapiens
21 SLCO4A1 28231
Affinity Capture-MS Homo sapiens
22 IFI16 3428
Affinity Capture-MS Homo sapiens
23 LSM11  
Affinity Capture-MS Homo sapiens
24 DNAJA1 3301
Affinity Capture-MS Homo sapiens
25 FLNB 2317
Affinity Capture-MS Homo sapiens
26 Eed  
Affinity Capture-MS Mus musculus
27 RPLP0 6175
Affinity Capture-MS Homo sapiens
28 HMGA1 3159
Affinity Capture-MS Homo sapiens
29 HELLS 3070
Affinity Capture-MS Homo sapiens
30 STIP1 10963
Affinity Capture-MS Homo sapiens
31 H3F3C 440093
Affinity Capture-MS Homo sapiens
32 Fancd2  
Affinity Capture-MS Mus musculus
33 WDR77 79084
Affinity Capture-MS Homo sapiens
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