Gene description for YTHDF1
Gene name YTH N(6)-methyladenosine RNA binding protein 1
Gene symbol YTHDF1
Other names/aliases C20orf21
Species Homo sapiens
 Database cross references - YTHDF1
ExoCarta ExoCarta_54915
Vesiclepedia VP_54915
Entrez Gene 54915
HGNC 15867
UniProt Q9BYJ9  
 YTHDF1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for YTHDF1
Molecular Function
    RNA binding GO:0003723 HDA
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    ribosome binding GO:0043022 IDA
    N6-methyladenosine-containing RNA reader activity GO:1990247 IBA
    N6-methyladenosine-containing RNA reader activity GO:1990247 IDA
Biological Process
    immune system process GO:0002376 IEA
    regulation of antigen processing and presentation GO:0002577 ISS
    learning GO:0007612 ISS
    memory GO:0007613 ISS
    stress granule assembly GO:0034063 IDA
    regulation of mRNA stability GO:0043488 IDA
    positive regulation of translation GO:0045727 IMP
    positive regulation of translational initiation GO:0045948 IDA
    positive regulation of translational initiation GO:0045948 IDA
    mRNA destabilization GO:0061157 IBA
    mRNA destabilization GO:0061157 IDA
    organelle assembly GO:0070925 IDA
    regulation of long-term synaptic potentiation GO:1900271 ISS
    regulation of axon guidance GO:1902667 ISS
Subcellular Localization
    P-body GO:0000932 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasmic stress granule GO:0010494 IDA
 Experiment description of studies that identified YTHDF1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for YTHDF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ISX  
Proximity Label-MS Homo sapiens
2 SNRPA 6626
Two-hybrid Homo sapiens
3 ST8SIA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LHFPL4  
Affinity Capture-MS Homo sapiens
5 UBE2H 7328
Affinity Capture-MS Homo sapiens
6 RPL26L1 51121
Affinity Capture-MS Homo sapiens
7 Pef1  
Two-hybrid Mus musculus
8 OR10H1  
Affinity Capture-MS Homo sapiens
9 DNAJC11 55735
Affinity Capture-MS Homo sapiens
10 NPRL2  
Affinity Capture-MS Homo sapiens
11 RBPMS 11030
Two-hybrid Homo sapiens
12 DNAJC8 22826
Affinity Capture-MS Homo sapiens
13 POT1  
Affinity Capture-MS Homo sapiens
14 RBMS3  
Two-hybrid Homo sapiens
15 SAR1B 51128
Affinity Capture-MS Homo sapiens
16 AES 166
Two-hybrid Homo sapiens
17 RPA2 6118
Affinity Capture-MS Homo sapiens
18 USP5 8078
Co-fractionation Homo sapiens
19 RPSAP58 388524
Affinity Capture-MS Homo sapiens
20 QKI 9444
Two-hybrid Homo sapiens
21 ZNF34  
Two-hybrid Homo sapiens
22 EMID1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 RPS19 6223
Affinity Capture-MS Homo sapiens
24 RPL10 6134
Affinity Capture-MS Homo sapiens
25 XRN2 22803
Two-hybrid Homo sapiens
26 KRTAP7-1  
Two-hybrid Homo sapiens
27 Ywhaz 22631
Two-hybrid Mus musculus
28 SOX2  
Affinity Capture-MS Homo sapiens
29 RPS3 6188
Affinity Capture-MS Homo sapiens
30 ANKRD29  
Affinity Capture-MS Homo sapiens
31 RPS20 6224
Proximity Label-MS Homo sapiens
32 KCNJ10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 MCM2 4171
Affinity Capture-MS Homo sapiens
34 SOGA1 140710
Affinity Capture-RNA Homo sapiens
35 NUP153 9972
Co-fractionation Homo sapiens
36 HEY1  
Two-hybrid Homo sapiens
37 NUP35 129401
Proximity Label-MS Homo sapiens
38 COQ3  
Affinity Capture-MS Homo sapiens
39 CPSF7 79869
Two-hybrid Homo sapiens
40 TMEM184B  
Affinity Capture-MS Homo sapiens
41 FBL 2091
Proximity Label-MS Homo sapiens
42 OIP5  
Two-hybrid Homo sapiens
43 GCM1  
Proximity Label-MS Homo sapiens
44 PRELP 5549
Affinity Capture-MS Homo sapiens
45 PRRC2B  
Two-hybrid Homo sapiens
46 FAM136A 84908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 SERPINH1 871
Co-fractionation Homo sapiens
48 FAF1 11124
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
49 SARNP 84324
Two-hybrid Homo sapiens
50 VPS4B 9525
Co-fractionation Homo sapiens
51 CLK2 1196
Two-hybrid Homo sapiens
52 MLC1 23209
Affinity Capture-MS Homo sapiens
53 C6orf15  
Two-hybrid Homo sapiens
54 Nsa2 59050
Two-hybrid Mus musculus
55 MYCN  
Affinity Capture-MS Homo sapiens
56 NPAS1  
Affinity Capture-MS Homo sapiens
57 RBM22 55696
Two-hybrid Homo sapiens
58 BATF2  
Two-hybrid Homo sapiens
59 RPL19 6143
Affinity Capture-MS Homo sapiens
60 SLC25A3 5250
Affinity Capture-MS Homo sapiens
61 KIF14 9928
Affinity Capture-MS Homo sapiens
62 KRTAP13-1  
Two-hybrid Homo sapiens
63 CTH 1491
Co-fractionation Homo sapiens
64 LIN28A  
Affinity Capture-MS Homo sapiens
65 AGO2 27161
Affinity Capture-MS Homo sapiens
66 KLF16  
Affinity Capture-MS Homo sapiens
67 DNASE2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 KRTAP8-1  
Two-hybrid Homo sapiens
69 FAM96A  
Affinity Capture-MS Homo sapiens
70 ZKSCAN8  
Affinity Capture-MS Homo sapiens
71 CMAS 55907
Affinity Capture-MS Homo sapiens
72 CDKN1A  
Affinity Capture-MS Homo sapiens
73 MEPCE 56257
Affinity Capture-MS Homo sapiens
74 ZMAT5  
Affinity Capture-MS Homo sapiens
75 RPS10 6204
Affinity Capture-MS Homo sapiens
76 MSX2  
Two-hybrid Homo sapiens
77 MICU2 221154
Affinity Capture-MS Homo sapiens
78 PURG  
Affinity Capture-MS Homo sapiens
79 CEP19  
Affinity Capture-MS Homo sapiens
80 ZNF76  
Affinity Capture-MS Homo sapiens
81 RPL5 6125
Affinity Capture-MS Homo sapiens
82 AKR7L 246181
Affinity Capture-MS Homo sapiens
83 Gtf2f1  
Two-hybrid Mus musculus
84 KIAA1683  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 FUT7  
Affinity Capture-MS Homo sapiens
86 LRSAM1 90678
Co-fractionation Homo sapiens
87 RPL31 6160
Affinity Capture-MS Homo sapiens
88 GLI3 2737
Proximity Label-MS Homo sapiens
89 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
90 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 MOV10 4343
Two-hybrid Homo sapiens
92 CLEC11A 6320
Affinity Capture-MS Homo sapiens
93 BICD2 23299
Two-hybrid Homo sapiens
94 FBXL6  
Affinity Capture-MS Homo sapiens
95 Bbs5  
Two-hybrid Mus musculus
96 TECR 9524
Affinity Capture-MS Homo sapiens
97 Ube2i  
Two-hybrid Mus musculus
98 ABHD11 83451
Two-hybrid Homo sapiens
99 ERI3 79033
Two-hybrid Homo sapiens
100 UBXN6 80700
Affinity Capture-MS Homo sapiens
101 HSPA12B 116835
Affinity Capture-MS Homo sapiens
102 CDIPT 10423
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 Nudt21 68219
Two-hybrid Mus musculus
104 PSG11  
Affinity Capture-MS Homo sapiens
105 Rbm14  
Two-hybrid Mus musculus
106 CCL22 6367
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 DYNC1I1  
Two-hybrid Homo sapiens
108 Grb2 14784
Two-hybrid Mus musculus
109 TMED9 54732
Two-hybrid Homo sapiens
110 SLC25A12 8604
Affinity Capture-MS Homo sapiens
111 ABT1 29777
Affinity Capture-MS Homo sapiens
112 Rbm38  
Two-hybrid Mus musculus
113 TRIM31  
Affinity Capture-MS Homo sapiens
114 RPS2 6187
Affinity Capture-MS Homo sapiens
115 OTUD1 220213
Affinity Capture-MS Homo sapiens
116 XPO1 7514
Affinity Capture-MS Homo sapiens
117 PIP 5304
Affinity Capture-MS Homo sapiens
118 RC3H2  
Affinity Capture-MS Homo sapiens
119 PTH2R  
Affinity Capture-MS Homo sapiens
120 KCTD12 115207
Affinity Capture-MS Homo sapiens
121 MGARP  
Affinity Capture-MS Homo sapiens
122 HSD3B7 80270
Proximity Label-MS Homo sapiens
123 CUL3 8452
Affinity Capture-MS Homo sapiens
124 CTTN 2017
Two-hybrid Homo sapiens
125 RPL3 6122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
126 EGFR 1956
Affinity Capture-MS Homo sapiens
127 DHX30 22907
Two-hybrid Homo sapiens
128 METTL21B  
Affinity Capture-MS Homo sapiens
129 SF1 7536
Two-hybrid Homo sapiens
130 Dynlrb1 67068
Two-hybrid Mus musculus
131 Acta1 11459
Two-hybrid Mus musculus
132 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
133 BLK 640
Affinity Capture-MS Homo sapiens
134 ACADSB 36
Affinity Capture-MS Homo sapiens
135 PPIL3 53938
Two-hybrid Homo sapiens
136 PRKCSH 5589
Affinity Capture-MS Homo sapiens
137 PSPC1 55269
Affinity Capture-MS Homo sapiens
138 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
139 SOHLH1  
Affinity Capture-MS Homo sapiens
140 SAV1  
Affinity Capture-MS Homo sapiens
141 RPS4Y1 6192
Affinity Capture-MS Homo sapiens
142 PSKH2  
Affinity Capture-MS Homo sapiens
143 ACOX3 8310
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
144 HSPA1A 3303
Affinity Capture-MS Homo sapiens
145 ZNF653  
Affinity Capture-MS Homo sapiens
146 PRKY  
Affinity Capture-MS Homo sapiens
147 RNF2  
Affinity Capture-MS Homo sapiens
148 BMI1  
Affinity Capture-MS Homo sapiens
149 Dynlt1b  
Two-hybrid Mus musculus
150 COQ4  
Affinity Capture-MS Homo sapiens
151 H2AFB2  
Affinity Capture-MS Homo sapiens
152 LPPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
153 YTHDF2 51441
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
154 AKAP1 8165
Proximity Label-MS Homo sapiens
155 Npm3  
Two-hybrid Mus musculus
156 RPS16 6217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
157 Mapre2 212307
Two-hybrid Mus musculus
158 IRAK2  
Affinity Capture-MS Homo sapiens
159 SLC25A11 8402
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
160 FADS3 3995
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
161 NXF1 10482
Affinity Capture-RNA Homo sapiens
162 HSF4  
Two-hybrid Homo sapiens
163 TP53 7157
Affinity Capture-MS Homo sapiens
164 VPS37C 55048
Two-hybrid Homo sapiens
165 IGHD  
Affinity Capture-MS Homo sapiens
166 Eif4a1 13681
Two-hybrid Mus musculus
167 Fbl 14113
Two-hybrid Mus musculus
168 SF3B4 10262
Co-fractionation Homo sapiens
169 ASPSCR1 79058
Co-fractionation Homo sapiens
170 RPA1 6117
Affinity Capture-MS Homo sapiens
171 DMRTB1  
Two-hybrid Homo sapiens
172 CTSG 1511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
173 PRR32  
Two-hybrid Homo sapiens
174 G3BP1 10146
Affinity Capture-MS Homo sapiens
175 C1orf35  
Affinity Capture-MS Homo sapiens
176 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
177 CPEB1  
Proximity Label-MS Homo sapiens
178 MKI67  
Affinity Capture-MS Homo sapiens
179 TUT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
180 YTHDF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
181 ESR1  
Affinity Capture-MS Homo sapiens
182 RBFOX2 23543
Two-hybrid Homo sapiens
183 ITSN2 50618
Two-hybrid Homo sapiens
184 NQO1 1728
Co-fractionation Homo sapiens
185 MAGED1 9500
Two-hybrid Homo sapiens
186 KRTAP26-1  
Two-hybrid Homo sapiens
187 GLUL 2752
Affinity Capture-MS Homo sapiens
188 RPSA 3921
Affinity Capture-MS Homo sapiens
189 Gnb2l1 14694
Two-hybrid Mus musculus
190 Rps15 20054
Two-hybrid Mus musculus
191 Fez2  
Two-hybrid Mus musculus
192 SLC9A3R1 9368
Co-fractionation Homo sapiens
193 TOLLIP 54472
Affinity Capture-MS Homo sapiens
194 SERBP1 26135
Proximity Label-MS Homo sapiens
195 FHIT 2272
Two-hybrid Homo sapiens
196 QRSL1  
Affinity Capture-MS Homo sapiens
197 TNFSF14  
Affinity Capture-MS Homo sapiens
198 MYC  
Affinity Capture-MS Homo sapiens
199 MFGE8 4240
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
200 SBK1  
Affinity Capture-MS Homo sapiens
201 C12orf57 113246
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which YTHDF1 is involved
No pathways found





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