Gene description for Acta1
Gene name actin, alpha 1, skeletal muscle
Gene symbol Acta1
Other names/aliases AA959943
Acta-2
Acts
Actsk-1
Species Mus musculus
 Database cross references - Acta1
ExoCarta ExoCarta_11459
Entrez Gene 11459
UniProt P68134  
 Acta1 identified in exosomes derived from the following tissue/cell type
Brain cancer cells 19109410    
Pancreatic cells 19351151    
 Gene ontology annotations for Acta1
Molecular Function
    ATP binding GO:0005524 IEA
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
Biological Process
    skeletal muscle thin filament assembly GO:0030240 ISO
    skeletal muscle fiber development GO:0048741 IDA
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    protein complex GO:0043234 ISO
    cytoskeleton GO:0005856 IEA
    blood microparticle GO:0072562 ISO
    stress fiber GO:0001725 ISO
    striated muscle thin filament GO:0005865 TAS
    actin cytoskeleton GO:0015629 ISO
    actin filament GO:0005884 ISO
    sarcomere GO:0030017 ISO
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified Acta1 in exosomes
1
Experiment ID 26
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|GAPDH|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Acta1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Acta1 is involved
PathwayEvidenceSource
Striated Muscle Contraction IEA Reactome





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