Gene description for RPS4Y1
Gene name ribosomal protein S4, Y-linked 1
Gene symbol RPS4Y1
Other names/aliases RPS4Y
S4
Species Homo sapiens
 Database cross references - RPS4Y1
ExoCarta ExoCarta_6192
Vesiclepedia VP_6192
Entrez Gene 6192
HGNC 10425
MIM 470000
UniProt P22090  
 RPS4Y1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for RPS4Y1
Molecular Function
    RNA binding GO:0003723 IBA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IMP
    rRNA binding GO:0019843 IEA
Biological Process
    translation GO:0006412 IBA
    translation GO:0006412 IMP
Subcellular Localization
    nucleus GO:0005634 HDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    ribosome GO:0005840 IDA
    membrane GO:0016020 HDA
    cytosolic small ribosomal subunit GO:0022627 IBA
    cytosolic small ribosomal subunit GO:0022627 NAS
 Experiment description of studies that identified RPS4Y1 in exosomes
1
Experiment ID 21
MISEV standards
EM|IEM
Biophysical techniques
Alix|TSG101|HSP70|CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for RPS4Y1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FTL 2512
Affinity Capture-MS Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
3 RPSAP58 388524
Affinity Capture-MS Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 SDC3  
Affinity Capture-MS Homo sapiens
6 RPS19 6223
Affinity Capture-MS Homo sapiens
7 AGPAT1 10554
Affinity Capture-MS Homo sapiens
8 KIF14 9928
Affinity Capture-MS Homo sapiens
9 ACOT7 11332
Affinity Capture-MS Homo sapiens
10 RPS4X 6191
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
11 YTHDF1 54915
Affinity Capture-MS Homo sapiens
12 PINK1  
Affinity Capture-MS Homo sapiens
13 ECT2 1894
Affinity Capture-MS Homo sapiens
14 PHYHIPL  
Affinity Capture-MS Homo sapiens
15 HECTD3 79654
Affinity Capture-MS Homo sapiens
16 ZNF746  
Affinity Capture-MS Homo sapiens
17 C1orf123 54987
Affinity Capture-MS Homo sapiens
18 ZZEF1 23140
Affinity Capture-MS Homo sapiens
19 WRAP53  
Affinity Capture-MS Homo sapiens
20 KIF20A 10112
Affinity Capture-MS Homo sapiens
21 RPS15 6209
Affinity Capture-MS Homo sapiens
22 ALDOA 226
Affinity Capture-MS Homo sapiens
23 CCT3 7203
Affinity Capture-MS Homo sapiens
24 CALM1 801
Reconstituted Complex Homo sapiens
25 WDR37 22884
Affinity Capture-MS Homo sapiens
26 SHMT2 6472
Affinity Capture-MS Homo sapiens
27 SLURP1  
Affinity Capture-MS Homo sapiens
28 UGT3A1  
Affinity Capture-MS Homo sapiens
29 SOX2  
Affinity Capture-MS Homo sapiens
30 ZC3H10  
Affinity Capture-MS Homo sapiens
31 RPS4Y2 140032
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
32 AURKB 9212
Affinity Capture-MS Homo sapiens
33 RPL10A 4736
Affinity Capture-MS Homo sapiens
34 RPS6 6194
Cross-Linking-MS (XL-MS) Homo sapiens
35 RBKS  
Affinity Capture-MS Homo sapiens
36 CHMP4C 92421
Affinity Capture-MS Homo sapiens
37 PADI4  
Affinity Capture-MS Homo sapiens
38 ACTR3B 57180
Affinity Capture-MS Homo sapiens
39 CHMP4B 128866
Affinity Capture-MS Homo sapiens
40 MYC  
Affinity Capture-MS Homo sapiens
41 LTV1  
Affinity Capture-MS Homo sapiens
42 KIF23 9493
Affinity Capture-MS Homo sapiens
43 SPRTN  
Affinity Capture-MS Homo sapiens
44 AK3 50808
Affinity Capture-MS Homo sapiens
45 CBX8 57332
Affinity Capture-MS Homo sapiens
46 ACTR3 10096
Affinity Capture-MS Homo sapiens
47 SSSCA1 10534
Affinity Capture-MS Homo sapiens
48 RPS8 6202
Affinity Capture-MS Homo sapiens
49 MEOX2  
Two-hybrid Homo sapiens
50 PRC1 9055
Affinity Capture-MS Homo sapiens
51 NEU2  
Affinity Capture-MS Homo sapiens
52 RPL23A 6147
Affinity Capture-MS Homo sapiens
53 NCAPD3 23310
Affinity Capture-MS Homo sapiens
54 RNF2  
Affinity Capture-MS Homo sapiens
55 WHSC1L1  
Affinity Capture-MS Homo sapiens
56 TMCC2  
Affinity Capture-MS Homo sapiens
57 CA5B 11238
Affinity Capture-MS Homo sapiens
58 CD55 1604
Affinity Capture-MS Homo sapiens
59 PRPS1 5631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 NEUROG3  
Affinity Capture-MS Homo sapiens
61 CEMIP 57214
Affinity Capture-MS Homo sapiens
62 CYLD  
Affinity Capture-MS Homo sapiens
63 HSPBP1 23640
Affinity Capture-MS Homo sapiens
64 AR 367
Affinity Capture-MS Homo sapiens
65 HINT3 135114
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RPS4Y1 is involved
PathwayEvidenceSource
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S TAS Reactome
Axon guidance IEA Reactome
Cap-dependent Translation Initiation TAS Reactome
Cellular response to starvation IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Eukaryotic Translation Elongation IEA Reactome
Eukaryotic Translation Initiation TAS Reactome
Eukaryotic Translation Termination IEA Reactome
Formation of a pool of free 40S subunits TAS Reactome
Formation of the ternary complex, and subsequently, the 43S complex TAS Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit TAS Reactome
Infectious disease TAS Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
L13a-mediated translational silencing of Ceruloplasmin expression TAS Reactome
Major pathway of rRNA processing in the nucleolus and cytosol TAS Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome
Metabolism of proteins TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA TAS Reactome
Nervous system development IEA Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) TAS Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) TAS Reactome
Nonsense-Mediated Decay (NMD) TAS Reactome
Peptide chain elongation IEA Reactome
Regulation of expression of SLITs and ROBOs IEA Reactome
Response of EIF2AK4 (GCN2) to amino acid deficiency IEA Reactome
Ribosomal scanning and start codon recognition TAS Reactome
rRNA processing TAS Reactome
rRNA processing in the nucleus and cytosol TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-1 Infection TAS Reactome
SARS-CoV-1 modulates host translation machinery TAS Reactome
SARS-CoV-1-host interactions TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2 modulates host translation machinery TAS Reactome
SARS-CoV-2-host interactions TAS Reactome
Selenoamino acid metabolism IEA Reactome
Selenocysteine synthesis IEA Reactome
Signaling by ROBO receptors IEA Reactome
SRP-dependent cotranslational protein targeting to membrane IEA Reactome
SRP-dependent cotranslational protein targeting to membrane TAS Reactome
Translation TAS Reactome
Translation IEA Reactome
Translation initiation complex formation TAS Reactome
Viral Infection Pathways TAS Reactome
Viral mRNA Translation TAS Reactome





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