Gene description for RPS4Y2
Gene name ribosomal protein S4, Y-linked 2
Gene symbol RPS4Y2
Other names/aliases RPS4Y2P
Species Homo sapiens
 Database cross references - RPS4Y2
ExoCarta ExoCarta_140032
Vesiclepedia VP_140032
Entrez Gene 140032
HGNC 18501
MIM 400030
UniProt Q8TD47  
 RPS4Y2 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for RPS4Y2
Molecular Function
    RNA binding GO:0003723 IBA
    structural constituent of ribosome GO:0003735 IBA
    rRNA binding GO:0019843 IEA
Biological Process
    translation GO:0006412 IBA
Subcellular Localization
    cytosolic small ribosomal subunit GO:0022627 IBA
 Experiment description of studies that identified RPS4Y2 in sEVs
1
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RPS4Y2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPSA 3921
Affinity Capture-MS Homo sapiens
2 FTL 2512
Affinity Capture-MS Homo sapiens
3 ANLN 54443
Affinity Capture-MS Homo sapiens
4 ZNF346  
Affinity Capture-MS Homo sapiens
5 RPSAP58 388524
Affinity Capture-MS Homo sapiens
6 SURF6  
Affinity Capture-MS Homo sapiens
7 ZNF428  
Affinity Capture-MS Homo sapiens
8 RPS19 6223
Affinity Capture-MS Homo sapiens
9 ABT1 29777
Affinity Capture-MS Homo sapiens
10 RPS3A 6189
Affinity Capture-MS Homo sapiens
11 RPL7A 6130
Affinity Capture-MS Homo sapiens
12 KIF14 9928
Affinity Capture-MS Homo sapiens
13 RPS2 6187
Affinity Capture-MS Homo sapiens
14 RPL13 6137
Affinity Capture-MS Homo sapiens
15 RPL3 6122
Affinity Capture-MS Homo sapiens
16 LIN28A  
Affinity Capture-MS Homo sapiens
17 ECT2 1894
Affinity Capture-MS Homo sapiens
18 ZNF668  
Affinity Capture-MS Homo sapiens
19 ZNF746  
Affinity Capture-MS Homo sapiens
20 FGFBP1 9982
Affinity Capture-MS Homo sapiens
21 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
22 DGCR8  
Affinity Capture-MS Homo sapiens
23 MFAP4 4239
Affinity Capture-MS Homo sapiens
24 KIF20A 10112
Affinity Capture-MS Homo sapiens
25 RPL14 9045
Affinity Capture-MS Homo sapiens
26 OIT3 170392
Affinity Capture-MS Homo sapiens
27 RPL4 6124
Affinity Capture-MS Homo sapiens
28 KLRG2 346689
Affinity Capture-MS Homo sapiens
29 CCDC97  
Affinity Capture-MS Homo sapiens
30 RBM4B  
Affinity Capture-MS Homo sapiens
31 RPS4X 6191
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
32 NEIL1  
Affinity Capture-MS Homo sapiens
33 AURKB 9212
Affinity Capture-MS Homo sapiens
34 KRR1 11103
Affinity Capture-MS Homo sapiens
35 MBNL1 4154
Affinity Capture-MS Homo sapiens
36 HIST1H2AM 8336
Affinity Capture-MS Homo sapiens
37 CHMP4C 92421
Affinity Capture-MS Homo sapiens
38 EMILIN1 11117
Affinity Capture-MS Homo sapiens
39 CHMP4B 128866
Affinity Capture-MS Homo sapiens
40 RPS10 6204
Affinity Capture-MS Homo sapiens
41 KIF23 9493
Affinity Capture-MS Homo sapiens
42 SET 6418
Affinity Capture-MS Homo sapiens
43 RPS4Y1 6192
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
44 SSSCA1 10534
Affinity Capture-MS Homo sapiens
45 RPS6 6194
Affinity Capture-MS Homo sapiens
46 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
47 PRC1 9055
Affinity Capture-MS Homo sapiens
48 CSNK1E 1454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
50 RPS8 6202
Affinity Capture-MS Homo sapiens
51 LYAR 55646
Affinity Capture-MS Homo sapiens
52 RPL36AL 6166
Affinity Capture-MS Homo sapiens
53 RPLP0 6175
Affinity Capture-MS Homo sapiens
54 LRRC46  
Affinity Capture-MS Homo sapiens
55 MAGEB2 4113
Affinity Capture-MS Homo sapiens
56 NEUROG3  
Affinity Capture-MS Homo sapiens
57 SRP14 6727
Affinity Capture-MS Homo sapiens
58 PRKRA 8575
Affinity Capture-MS Homo sapiens
59 DAXX  
Affinity Capture-MS Homo sapiens
60 MRPL2 51069
Affinity Capture-MS Homo sapiens
61 RPS16 6217
Affinity Capture-MS Homo sapiens
62 RPL19 6143
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RPS4Y2 is involved
PathwayEvidenceSource
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S TAS Reactome
Axon guidance IEA Reactome
Cap-dependent Translation Initiation TAS Reactome
Cellular response to starvation IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Eukaryotic Translation Elongation IEA Reactome
Eukaryotic Translation Initiation TAS Reactome
Eukaryotic Translation Termination IEA Reactome
Formation of a pool of free 40S subunits TAS Reactome
Formation of the ternary complex, and subsequently, the 43S complex TAS Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit TAS Reactome
Infectious disease TAS Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
L13a-mediated translational silencing of Ceruloplasmin expression TAS Reactome
Major pathway of rRNA processing in the nucleolus and cytosol TAS Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome
Metabolism of proteins TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA TAS Reactome
Nervous system development IEA Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) TAS Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) TAS Reactome
Nonsense-Mediated Decay (NMD) TAS Reactome
Peptide chain elongation IEA Reactome
Regulation of expression of SLITs and ROBOs IEA Reactome
Response of EIF2AK4 (GCN2) to amino acid deficiency IEA Reactome
Ribosomal scanning and start codon recognition TAS Reactome
rRNA processing TAS Reactome
rRNA processing in the nucleus and cytosol TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-1 Infection TAS Reactome
SARS-CoV-1 modulates host translation machinery TAS Reactome
SARS-CoV-1-host interactions TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2 modulates host translation machinery TAS Reactome
SARS-CoV-2-host interactions TAS Reactome
Selenoamino acid metabolism IEA Reactome
Selenocysteine synthesis IEA Reactome
Signaling by ROBO receptors IEA Reactome
SRP-dependent cotranslational protein targeting to membrane IEA Reactome
SRP-dependent cotranslational protein targeting to membrane TAS Reactome
Translation TAS Reactome
Translation IEA Reactome
Translation initiation complex formation TAS Reactome
Viral Infection Pathways TAS Reactome
Viral mRNA Translation TAS Reactome





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