Gene description for AK3
Gene name adenylate kinase 3
Gene symbol AK3
Other names/aliases AK3L1
AK6
AKL3L
AKL3L1
FIX
Species Homo sapiens
 Database cross references - AK3
ExoCarta ExoCarta_50808
Vesiclepedia VP_50808
Entrez Gene 50808
HGNC 17376
MIM 609290
UniProt Q9UIJ7  
 AK3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
 Gene ontology annotations for AK3
Molecular Function
    adenylate kinase activity GO:0004017 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    GTP binding GO:0005525 IEA
    ligase activity GO:0016874 IEA
    nucleoside triphosphate adenylate kinase activity GO:0046899 EXP
    nucleoside triphosphate adenylate kinase activity GO:0046899 IBA
    nucleoside triphosphate adenylate kinase activity GO:0046899 IDA
Biological Process
    ADP biosynthetic process GO:0006172 IEA
    blood coagulation GO:0007596 TAS
    nucleoside triphosphate biosynthetic process GO:0009142 IBA
    AMP metabolic process GO:0046033 IBA
    AMP metabolic process GO:0046033 IDA
    GTP metabolic process GO:0046039 IDA
    ITP metabolic process GO:0046041 IDA
    UTP metabolic process GO:0046051 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISS
    mitochondrial matrix GO:0005759 IBA
    mitochondrial matrix GO:0005759 ISS
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified AK3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for AK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 COA4  
Co-fractionation Homo sapiens
3 AK4 205
Co-fractionation Homo sapiens
4 CLPP 8192
Proximity Label-MS Homo sapiens
5 SUMF2 25870
Co-fractionation Homo sapiens
6 FAHD1 81889
Co-fractionation Homo sapiens
7 GABARAPL2 11345
Co-fractionation Homo sapiens
8 COX17  
Co-fractionation Homo sapiens
9 ACAT1 38
Co-fractionation Homo sapiens
10 TXN2 25828
Co-fractionation Homo sapiens
11 TSFM 10102
Proximity Label-MS Homo sapiens
12 SOD2 6648
Co-fractionation Homo sapiens
13 AK2 204
Co-fractionation Homo sapiens
14 IBA57  
Affinity Capture-MS Homo sapiens
15 GIMAP8 155038
Co-fractionation Homo sapiens
16 KRTAP10-8  
Two-hybrid Homo sapiens
17 MSRB2  
Co-fractionation Homo sapiens
18 TRIAP1  
Co-fractionation Homo sapiens
19 PKM 5315
Co-fractionation Homo sapiens
20 MDH2 4191
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
21 ACAA1 30
Co-fractionation Homo sapiens
22 ISOC2  
Co-fractionation Homo sapiens
23 ZDHHC17 23390
Two-hybrid Homo sapiens
24 LGALS3 3958
Co-fractionation Homo sapiens
25 ACAA2 10449
Co-fractionation Homo sapiens
26 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
27 PRDX5 25824
Co-fractionation Homo sapiens
28 MSRB3 253827
Co-fractionation Homo sapiens
29 PPIA 5478
Co-fractionation Homo sapiens
30 TXNDC12 51060
Co-fractionation Homo sapiens
31 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
32 CFL1 1072
Co-fractionation Homo sapiens
33 PARK7 11315
Co-fractionation Homo sapiens
34 C21orf33  
Proximity Label-MS Homo sapiens
35 TRIM54  
Two-hybrid Homo sapiens
36 APPBP2  
Two-hybrid Homo sapiens
37 DAPK3 1613
Two-hybrid Homo sapiens
38 TXN 7295
Co-fractionation Homo sapiens
39 TBRG4 9238
Proximity Label-MS Homo sapiens
40 AARS2  
Proximity Label-MS Homo sapiens
41 NUDT19 390916
Co-fractionation Homo sapiens
42 RAB7A 7879
Co-fractionation Homo sapiens
43 PTRHD1 391356
Co-fractionation Homo sapiens
44 RPS14 6208
Co-fractionation Homo sapiens
45 CYB5R3 1727
Co-fractionation Homo sapiens
46 ACO2 50
Co-fractionation Homo sapiens
47 PDHA1 5160
Proximity Label-MS Homo sapiens
48 CTSB 1508
Co-fractionation Homo sapiens
49 RPS4Y1 6192
Affinity Capture-MS Homo sapiens
50 FXN  
Co-fractionation Homo sapiens
51 LYPLA1 10434
Co-fractionation Homo sapiens
52 AK1 203
Co-fractionation Homo sapiens
53 RANBP2 5903
Co-fractionation Homo sapiens
54 HNRNPUL2 221092
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which AK3 is involved
PathwayEvidenceSource
Factors involved in megakaryocyte development and platelet production TAS Reactome
Hemostasis TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here