Gene description for ACAT1
Gene name acetyl-CoA acetyltransferase 1
Gene symbol ACAT1
Other names/aliases ACAT
MAT
T2
THIL
Species Homo sapiens
 Database cross references - ACAT1
ExoCarta ExoCarta_38
Entrez Gene 38
HGNC 93
MIM 607809
UniProt P24752  
 ACAT1 identified in exosomes derived from the following tissue/cell type
Squamous carcinoma cells 20124223    
Urine 19056867    
 Gene ontology annotations for ACAT1
Molecular Function
    metal ion binding GO:0046872 IEA
    protein homodimerization activity GO:0042803 IEA
    enzyme binding GO:0019899 IEA
    acetyl-CoA C-acetyltransferase activity GO:0003985 EXP
    coenzyme binding GO:0050662 IEA
Biological Process
    branched-chain amino acid catabolic process GO:0009083 TAS
    response to hormone GO:0009725 IEA
    liver development GO:0001889 IEA
    brain development GO:0007420 IEA
    cellular ketone body metabolic process GO:0046950 TAS
    adipose tissue development GO:0060612 IEA
    ketone body catabolic process GO:0046952 TAS
    cellular nitrogen compound metabolic process GO:0034641 TAS
    protein homooligomerization GO:0051260 IEA
    response to starvation GO:0042594 IEA
    cellular lipid metabolic process GO:0044255 TAS
    metanephric proximal convoluted tubule development GO:0072229 IEA
    ketone body biosynthetic process GO:0046951 TAS
    small molecule metabolic process GO:0044281 TAS
    response to organic cyclic compound GO:0014070 IEA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    mitochondrial inner membrane GO:0005743 IEA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified ACAT1 in exosomes
1
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ACAT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GC20  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ACAT1 is involved
PathwayEvidenceSource
Branched-chain amino acid catabolism TAS Reactome
Synthesis of Ketone Bodies TAS Reactome
Utilization of Ketone Bodies TAS Reactome





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