Gene description for TMEM65
Gene name transmembrane protein 65
Gene symbol TMEM65
Other names/aliases -
Species Homo sapiens
 Database cross references - TMEM65
ExoCarta ExoCarta_157378
Vesiclepedia VP_157378
Entrez Gene 157378
HGNC 25203
UniProt Q6PI78  
 TMEM65 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for TMEM65
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cardiac ventricle development GO:0003231 IBA
    cardiac ventricle development GO:0003231 ISS
    cardiac conduction GO:0061337 ISS
    regulation of cardiac conduction GO:1903779 IBA
    regulation of cardiac conduction GO:1903779 ISS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrial inner membrane GO:0005743 IDA
    plasma membrane GO:0005886 IDA
    intercalated disc GO:0014704 IDA
 Experiment description of studies that identified TMEM65 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TMEM65
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSD17B11 51170
Two-hybrid Homo sapiens
2 GJA8  
Two-hybrid Homo sapiens
3 ACAT1 38
Co-fractionation Homo sapiens
4 SLC10A6  
Two-hybrid Homo sapiens
5 TMEM80  
Two-hybrid Homo sapiens
6 ZFYVE27 118813
Two-hybrid Homo sapiens
7 SRPRB 58477
Co-fractionation Homo sapiens
8 TM4SF18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 JPH1 56704
Two-hybrid Homo sapiens
10 ACAA2 10449
Co-fractionation Homo sapiens
11 ERGIC3 51614
Two-hybrid Homo sapiens
12 DNAJA3 9093
Proximity Label-MS Homo sapiens
13 ABHD16A 7920
Two-hybrid Homo sapiens
14 S100A10 6281
Co-fractionation Homo sapiens
15 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
16 REEP4  
Two-hybrid Homo sapiens
17 EBP  
Two-hybrid Homo sapiens
18 JAGN1 84522
Two-hybrid Homo sapiens
19 RTN4 57142
Co-fractionation Homo sapiens
20 SFXN1 94081
Proximity Label-MS Homo sapiens
21 FAM209A 200232
Two-hybrid Homo sapiens
22 PTGES 9536
Two-hybrid Homo sapiens
23 GPR42  
Two-hybrid Homo sapiens
24 TAX1BP3 30851
Co-fractionation Homo sapiens
25 FAM134C 162427
Two-hybrid Homo sapiens
26 COQ9  
Affinity Capture-MS Homo sapiens
27 MPZL1 9019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TIMMDC1  
Two-hybrid Homo sapiens
29 FAM174A 345757
Two-hybrid Homo sapiens
30 FBXW7  
Synthetic Lethality Homo sapiens
31 PDZK1IP1 10158
Two-hybrid Homo sapiens
32 ARL13B 200894
Two-hybrid Homo sapiens
33 SCN3B  
Two-hybrid Homo sapiens
34 IL3RA  
Two-hybrid Homo sapiens
35 NDUFS6  
Co-fractionation Homo sapiens
36 TMX2 51075
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which TMEM65 is involved
No pathways found





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