Gene description for DNAJA3
Gene name DnaJ (Hsp40) homolog, subfamily A, member 3
Gene symbol DNAJA3
Other names/aliases HCA57
TID1
hTID-1
Species Homo sapiens
 Database cross references - DNAJA3
ExoCarta ExoCarta_9093
Vesiclepedia VP_9093
Entrez Gene 9093
HGNC 11808
MIM 608382
UniProt Q96EY1  
 DNAJA3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for DNAJA3
Molecular Function
    NF-kappaB binding GO:0051059 IPI
    interferon-gamma receptor binding GO:0005133 IDA
    transcription factor binding GO:0008134 IPI
    protein kinase binding GO:0019901 IPI
    receptor tyrosine kinase binding GO:0030971 IEA
    unfolded protein binding GO:0051082 IBA
    chaperone binding GO:0051087 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
    Hsp70 protein binding GO:0030544 IEA
Biological Process
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 IDA
    negative regulation of interferon-gamma-mediated signaling pathway GO:0060336 IDA
    protein stabilization GO:0050821 IDA
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 IDA
    skeletal muscle acetylcholine-gated channel clustering GO:0071340 ISS
    positive regulation of T cell proliferation GO:0042102 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    protein refolding GO:0042026 IBA
    mitochondrion organization GO:0007005 IBA
    negative regulation of programmed cell death GO:0043069 IEA
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IDA
    negative regulation of cell proliferation GO:0008285 IDA
    negative regulation of apoptotic process GO:0043066 IDA
    response to interferon-gamma GO:0034341 IDA
    positive regulation of apoptotic process GO:0043065 IDA
    small GTPase mediated signal transduction GO:0007264 IEA
    protein folding GO:0006457 IDA
    activation-induced cell death of T cells GO:0006924 IEA
    mitochondrial DNA replication GO:0006264 IEA
    negative regulation of protein kinase activity GO:0006469 IDA
    response to heat GO:0009408 IEA
    T cell differentiation in thymus GO:0033077 IEA
    neuromuscular junction development GO:0007528 IDA
    cell aging GO:0007569 IEA
    positive regulation of protein ubiquitination GO:0031398 IDA
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IMP
    I-kappaB/NF-kappaB complex GO:0033256 IDA
    neuromuscular junction GO:0031594 ISS
    mitochondrial nucleoid GO:0042645 IDA
    cell junction GO:0030054 IEA
    mitochondrion GO:0005739 IDA
    IkappaB kinase complex GO:0008385 IDA
    actin filament GO:0005884 IDA
    nucleus GO:0005634 IEA
    extrinsic component of plasma membrane GO:0019897 ISS
    postsynaptic membrane GO:0045211 ISS
    mitochondrial matrix GO:0005759 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified DNAJA3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DNAJA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RASA1 5921
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
2 RNF34  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 ZNF297  
Two-hybrid Homo sapiens
4 FLJ36766  
Two-hybrid Homo sapiens
5 BRCA1 672
Protein-peptide Homo sapiens
6 DMC1  
Co-localization Homo sapiens
7 Jak2 16452
Two-hybrid Mus musculus
8 JAK2 3717
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 SMAD9  
Affinity Capture-MS Homo sapiens
10 RAD51  
Co-localization Homo sapiens
11 HSPA1A  
Affinity Capture-MS Homo sapiens
12 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 IFNGR2  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which DNAJA3 is involved
No pathways found





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