Gene description for NDUFA12
Gene name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12
Gene symbol NDUFA12
Other names/aliases B17.2
DAP13
Species Homo sapiens
 Database cross references - NDUFA12
ExoCarta ExoCarta_55967
Vesiclepedia VP_55967
Entrez Gene 55967
HGNC 23987
MIM 614530
UniProt Q9UI09  
 NDUFA12 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NDUFA12
Molecular Function
    protein binding GO:0005515 IPI
    NADH dehydrogenase (ubiquinone) activity GO:0008137 NAS
Biological Process
    response to oxidative stress GO:0006979 IBA
    aerobic respiration GO:0009060 NAS
    mitochondrial respiratory chain complex I assembly GO:0032981 IEA
    mitochondrial ATP synthesis coupled electron transport GO:0042775 IMP
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    cytosol GO:0005829 IDA
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IDA
    respiratory chain complex I GO:0045271 IMP
 Experiment description of studies that identified NDUFA12 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NDUFA12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLIRP 81892
Proximity Label-MS Homo sapiens
2 Tmed2 56334
Affinity Capture-MS Mus musculus
3 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
4 NDUFB8 4714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SDHB 6390
Co-fractionation Homo sapiens
6 VAPA 9218
Affinity Capture-MS Homo sapiens
7 TSHZ2  
Affinity Capture-MS Homo sapiens
8 MYO15B 80022
Two-hybrid Homo sapiens
9 TRUB2  
Proximity Label-MS Homo sapiens
10 MTG2  
Proximity Label-MS Homo sapiens
11 NGRN  
Proximity Label-MS Homo sapiens
12 CHCHD1  
Proximity Label-MS Homo sapiens
13 NTNG1  
Affinity Capture-MS Homo sapiens
14 UQCRB 7381
Co-fractionation Homo sapiens
15 GPR107 57720
Affinity Capture-MS Homo sapiens
16 SYT4  
Two-hybrid Homo sapiens
17 NDUFA4 4697
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 GJA8  
Affinity Capture-MS Homo sapiens
19 DNAJC15  
Proximity Label-MS Homo sapiens
20 MTRF1  
Proximity Label-MS Homo sapiens
21 GFM2 84340
Proximity Label-MS Homo sapiens
22 CCDC90B  
Proximity Label-MS Homo sapiens
23 TOMM22 56993
Co-fractionation Homo sapiens
24 ABCD1 215
Co-fractionation Homo sapiens
25 LRPPRC 10128
Proximity Label-MS Homo sapiens
26 FOXA1  
Affinity Capture-MS Homo sapiens
27 NDUFB9 4715
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 NDUFS3 4722
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 CRYZ 1429
Proximity Label-MS Homo sapiens
30 TFAM 7019
Proximity Label-MS Homo sapiens
31 PMPCA 23203
Proximity Label-MS Homo sapiens
32 HINT2 84681
Proximity Label-MS Homo sapiens
33 NDUFA7 4701
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 TIMMDC1  
Affinity Capture-MS Homo sapiens
35 C8orf82  
Proximity Label-MS Homo sapiens
36 NDUFA5 4698
Affinity Capture-MS Homo sapiens
37 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 MRPS26 64949
Proximity Label-MS Homo sapiens
39 SURF1  
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
40 EXD2  
Proximity Label-MS Homo sapiens
41 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
42 NDUFAF1  
Affinity Capture-MS Homo sapiens
43 TRMT1 55621
Affinity Capture-MS Homo sapiens
44 NDUFV3 4731
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ATP5H 10476
Co-fractionation Homo sapiens
46 WIF1 11197
Affinity Capture-MS Homo sapiens
47 PMPCB 9512
Proximity Label-MS Homo sapiens
48 NDUFA11 126328
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 FASTKD2  
Proximity Label-MS Homo sapiens
50 NDUFA6  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
51 VWA8 23078
Proximity Label-MS Homo sapiens
52 NDUFS5 4725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 SLC25A3 5250
Co-fractionation Homo sapiens
54 MDH2 4191
Proximity Label-MS Homo sapiens
55 SLC22A4 6583
Affinity Capture-MS Homo sapiens
56 CYP2J2  
Affinity Capture-MS Homo sapiens
57 EXTL3 2137
Affinity Capture-MS Homo sapiens
58 MPV17 4358
Co-fractionation Homo sapiens
59 C6orf203  
Proximity Label-MS Homo sapiens
60 NDUFS4 4724
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 COX15 1355
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
62 FASTKD5  
Proximity Label-MS Homo sapiens
63 NDUFB7 4713
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 ND5 4540
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 EMP3 2014
Affinity Capture-MS Homo sapiens
66 NDUFA8 4702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 IL1R2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 TUFM 7284
Proximity Label-MS Homo sapiens
69 HSCB 150274
Proximity Label-MS Homo sapiens
70 GALNT6 11226
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 GDPD5 81544
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 CCDC109B 55013
Proximity Label-MS Homo sapiens
73 NDUFA2 4695
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
74 CYC1 1537
Co-fractionation Homo sapiens
75 UQCRC1 7384
Affinity Capture-MS Homo sapiens
76 RPN1 6184
Co-fractionation Homo sapiens
77 MCU 90550
Proximity Label-MS Homo sapiens
78 MRPL11 65003
Proximity Label-MS Homo sapiens
79 MTRF1L  
Proximity Label-MS Homo sapiens
80 DNAJC30  
Affinity Capture-MS Homo sapiens
81 VDAC2 7417
Co-fractionation Homo sapiens
82 ATP5B 506
Co-fractionation Homo sapiens
83 NDUFV1 4723
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 CRELD1 78987
Affinity Capture-MS Homo sapiens
85 ND1  
Affinity Capture-MS Homo sapiens
86 CISD1 55847
Co-fractionation Homo sapiens
87 GPIHBP1  
Affinity Capture-MS Homo sapiens
88 Rab5c 19345
Affinity Capture-MS Mus musculus
89 C15orf48  
Affinity Capture-MS Homo sapiens
90 OTC 5009
Proximity Label-MS Homo sapiens
91 CYB5B 80777
Co-fractionation Homo sapiens
92 MRRF  
Proximity Label-MS Homo sapiens
93 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 MRPS12  
Proximity Label-MS Homo sapiens
95 MTIF2 4528
Proximity Label-MS Homo sapiens
96 NDUFB10 4716
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
97 NDUFA13 51079
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 NDUFA9 4704
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 CLPP 8192
Proximity Label-MS Homo sapiens
100 MTIF3  
Proximity Label-MS Homo sapiens
101 UQCRC2 7385
Co-fractionation Homo sapiens
102 TMED8 283578
Two-hybrid Homo sapiens
103 Bmpr1a  
Affinity Capture-MS Mus musculus
104 GOLT1B 51026
Affinity Capture-MS Homo sapiens
105 Rmdn3  
Affinity Capture-MS Mus musculus
106 RMND1 55005
Proximity Label-MS Homo sapiens
107 ATP1B3 483
Co-fractionation Homo sapiens
108 AQP3  
Affinity Capture-MS Homo sapiens
109 C17orf80 55028
Proximity Label-MS Homo sapiens
110 MRPL15 29088
Co-fractionation Homo sapiens
111 C12orf65  
Proximity Label-MS Homo sapiens
112 TACO1  
Proximity Label-MS Homo sapiens
113 MTG1  
Proximity Label-MS Homo sapiens
114 RPUSD4 84881
Proximity Label-MS Homo sapiens
115 NDUFB3 4709
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 APOOL 139322
Affinity Capture-MS Homo sapiens
117 COX6C 1345
Affinity Capture-MS Homo sapiens
118 DHX30 22907
Proximity Label-MS Homo sapiens
119 NDUFB6 4712
Affinity Capture-MS Homo sapiens
120 ATP1B1 481
Co-fractionation Homo sapiens
121 ACAD9 28976
Proximity Label-MS Homo sapiens
122 NDUFS1 4719
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
123 NDUFA10 4705
Co-fractionation Homo sapiens
124 NDUFA1 4694
Affinity Capture-MS Homo sapiens
125 HSPA9 3313
Proximity Label-MS Homo sapiens
126 NDUFV2 4729
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 NDUFC2 4718
Affinity Capture-MS Homo sapiens
128 METTL15  
Proximity Label-MS Homo sapiens
129 BAIAP2 10458
Affinity Capture-MS Homo sapiens
130 MCUR1 63933
Proximity Label-MS Homo sapiens
131 NDUFS2 4720
Affinity Capture-MS Homo sapiens
132 IMMT 10989
Affinity Capture-MS Homo sapiens
133 MTERF3  
Proximity Label-MS Homo sapiens
134 DNAJC28  
Proximity Label-MS Homo sapiens
135 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
136 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
137 TSFM 10102
Proximity Label-MS Homo sapiens
138 AUH 549
Proximity Label-MS Homo sapiens
139 TBRG4 9238
Proximity Label-MS Homo sapiens
140 DNAJC19 131118
Proximity Label-MS Homo sapiens
141 ERLIN1 10613
Co-fractionation Homo sapiens
142 C3AR1  
Affinity Capture-MS Homo sapiens
143 ICT1 3396
Proximity Label-MS Homo sapiens
144 UQCRH 7388
Affinity Capture-MS Homo sapiens
145 LONP1 9361
Proximity Label-MS Homo sapiens
146 PDHA1 5160
Proximity Label-MS Homo sapiens
147 DNAJA3 9093
Proximity Label-MS Homo sapiens
148 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 COX8A  
Proximity Label-MS Homo sapiens
150 E2F4  
Affinity Capture-MS Homo sapiens
151 C21orf33  
Proximity Label-MS Homo sapiens
152 SSBP1 6742
Proximity Label-MS Homo sapiens
153 MMGT1 93380
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
154 TMEM70  
Proximity Label-MS Homo sapiens
155 FASTKD3  
Proximity Label-MS Homo sapiens
156 NDUFS8 4728
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
157 GFM1 85476
Proximity Label-MS Homo sapiens
158 AARS2  
Proximity Label-MS Homo sapiens
159 TEFM  
Proximity Label-MS Homo sapiens
160 ZNF768 79724
Two-hybrid Homo sapiens
161 SLC25A51  
Proximity Label-MS Homo sapiens
162 RPUSD3  
Proximity Label-MS Homo sapiens
163 GRSF1 2926
Proximity Label-MS Homo sapiens
164 C1QBP 708
Proximity Label-MS Homo sapiens
165 BAD  
Affinity Capture-MS Homo sapiens
166 ATP5C1 509
Co-fractionation Homo sapiens
167 NDUFB11 54539
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
168 NDUFB5 4711
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
169 METTL17  
Proximity Label-MS Homo sapiens
170 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
171 YBEY  
Affinity Capture-MS Homo sapiens
172 NDUFS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
173 DDX28  
Proximity Label-MS Homo sapiens
174 MTCH2 23788
Co-fractionation Homo sapiens
175 NDUFB4 4710
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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