Gene description for COX6C
Gene name cytochrome c oxidase subunit VIc
Gene symbol COX6C
Other names/aliases -
Species Homo sapiens
 Database cross references - COX6C
ExoCarta ExoCarta_1345
Vesiclepedia VP_1345
Entrez Gene 1345
HGNC 2285
MIM 124090
UniProt P09669  
 COX6C identified in sEVs derived from the following tissue/cell type
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Mesenchymal stem cells 36408942    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for COX6C
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    generation of precursor metabolites and energy GO:0006091 TAS
    mitochondrial electron transport, cytochrome c to oxygen GO:0006123 NAS
    cellular respiration GO:0045333 NAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrial intermembrane space GO:0005758 TAS
    mitochondrial membrane GO:0031966 IDA
    respiratory chain complex IV GO:0045277 IEA
 Experiment description of studies that identified COX6C in sEVs
1
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for COX6C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 COX5A 9377
Co-fractionation Homo sapiens
3 DNAJC11 55735
Co-fractionation Homo sapiens
4 VAPA 9218
Affinity Capture-MS Homo sapiens
5 CRK 1398
Affinity Capture-MS Homo sapiens
6 NDUFB5 4711
Co-fractionation Homo sapiens
7 NDUFA12 55967
Affinity Capture-MS Homo sapiens
8 CALM1 801
Affinity Capture-MS Homo sapiens
9 NDUFA4 4697
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
10 FFAR1  
Affinity Capture-MS Homo sapiens
11 NDUFS2 4720
Co-fractionation Homo sapiens
12 NDUFB9 4715
Co-fractionation Homo sapiens
13 NDUFS3 4722
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
14 TFAM 7019
Co-fractionation Homo sapiens
15 COQ9  
Affinity Capture-MS Homo sapiens
16 COX7A2L 9167
Co-fractionation Homo sapiens
17 CHCHD2  
Affinity Capture-MS Homo sapiens
18 PRC1 9055
Affinity Capture-MS Homo sapiens
19 NDUFA5 4698
Co-fractionation Homo sapiens
20 CHCHD10  
Affinity Capture-MS Homo sapiens
21 HADHA 3030
Co-fractionation Homo sapiens
22 COX7B2  
Affinity Capture-MS Homo sapiens
23 MINOS1  
Co-fractionation Homo sapiens
24 C20orf24  
Co-fractionation Homo sapiens
25 LACTB 114294
Co-fractionation Homo sapiens
26 PHB 5245
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
27 ATP5H 10476
Co-fractionation Homo sapiens
28 CALR 811
Co-fractionation Homo sapiens
29 NDUFS5 4725
Co-fractionation Homo sapiens
30 Lima1  
Affinity Capture-MS Mus musculus
31 KLF16  
Affinity Capture-MS Homo sapiens
32 APOO 79135
Proximity Label-MS Homo sapiens
33 E2F4  
Affinity Capture-MS Homo sapiens
34 MTCH2 23788
Co-fractionation Homo sapiens
35 P4HA1 5033
Co-fractionation Homo sapiens
36 NDUFS4 4724
Co-fractionation Homo sapiens
37 SURF1  
Proximity Label-MS Homo sapiens
38 STOM 2040
Co-fractionation Homo sapiens
39 HSP90B1 7184
Co-fractionation Homo sapiens
40 CYB5A 1528
Co-fractionation Homo sapiens
41 NELFE 7936
Affinity Capture-MS Homo sapiens
42 CYC1 1537
Co-fractionation Homo sapiens
43 RPA4  
Proximity Label-MS Homo sapiens
44 COX7B 1349
Co-fractionation Homo sapiens
45 DNAJC30  
Proximity Label-MS Homo sapiens
46 SAT1  
Two-hybrid Homo sapiens
47 TMEM70  
Proximity Label-MS Homo sapiens
48 NIPSNAP1 8508
Co-fractionation Homo sapiens
49 SLC25A5 292
Cross-Linking-MS (XL-MS) Homo sapiens
50 COX4I1 1327
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
51 CISD3 284106
Affinity Capture-MS Homo sapiens
52 PTN  
Two-hybrid Homo sapiens
53 C15orf48  
Affinity Capture-MS Homo sapiens
54 SLC25A24 29957
Co-fractionation Homo sapiens
55 HADHB 3032
Co-fractionation Homo sapiens
56 SLC25A12 8604
Proximity Label-MS Homo sapiens
57 VAPB 9217
Co-fractionation Homo sapiens
58 CANX 821
Co-fractionation Homo sapiens
59 NDUFB10 4716
Co-fractionation Homo sapiens
60 SOX6  
Affinity Capture-MS Homo sapiens
61 NDUFA9 4704
Co-fractionation Homo sapiens
62 NELFCD 51497
Affinity Capture-MS Homo sapiens
63 RMND1 55005
Proximity Label-MS Homo sapiens
64 BCAP31 10134
Co-fractionation Homo sapiens
65 COX6A1  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
66 EGFR 1956
Negative Genetic Homo sapiens
67 COX6B1 1340
Affinity Capture-MS Homo sapiens
68 OCIAD1 54940
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
69 SMDT1  
Co-fractionation Homo sapiens
70 NDUFB3 4709
Co-fractionation Homo sapiens
71 RPA3 6119
Proximity Label-MS Homo sapiens
72 APOOL 139322
Affinity Capture-MS Homo sapiens
73 COX2 4513
Co-fractionation Homo sapiens
74 SCCPDH 51097
Co-fractionation Homo sapiens
75 GBAS 2631
Co-fractionation Homo sapiens
76 COX7A1  
Affinity Capture-MS Homo sapiens
77 CLPP 8192
Proximity Label-MS Homo sapiens
78 AUH 549
Co-fractionation Homo sapiens
79 COX4I2 84701
Affinity Capture-MS Homo sapiens
80 BCKDHA 593
Co-fractionation Homo sapiens
81 PDHA1 5160
Co-fractionation Homo sapiens
82 CAPZA2 830
Affinity Capture-MS Homo sapiens
83 Htt  
Affinity Capture-MS Mus musculus
84 PDHB 5162
Co-fractionation Homo sapiens
85 COX8A  
Co-fractionation Homo sapiens
86 RCHY1  
Two-hybrid Homo sapiens
87 SFXN1 94081
Proximity Label-MS Homo sapiens
88 VDAC2 7417
Co-fractionation Homo sapiens
89 COQ2  
Affinity Capture-MS Homo sapiens
90 RAB2A 5862
Affinity Capture-MS Homo sapiens
91 SLC25A51  
Proximity Label-MS Homo sapiens
92 KRAS 3845
Synthetic Lethality Homo sapiens
93 COX7C 1350
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
94 RAC1 5879
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 VDAC3 7419
Co-fractionation Homo sapiens
96 MGST3 4259
Co-fractionation Homo sapiens
97 NDUFS7 374291
Co-fractionation Homo sapiens
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