Gene description for MGST3
Gene name microsomal glutathione S-transferase 3
Gene symbol MGST3
Other names/aliases GST-III
Species Homo sapiens
 Database cross references - MGST3
ExoCarta ExoCarta_4259
Vesiclepedia VP_4259
Entrez Gene 4259
HGNC 7064
MIM 604564
UniProt O14880  
 MGST3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for MGST3
Molecular Function
    glutathione transferase activity GO:0004364 IDA
    glutathione transferase activity GO:0004364 IDA
    leukotriene-C4 synthase activity GO:0004464 IDA
    glutathione peroxidase activity GO:0004602 IBA
    glutathione peroxidase activity GO:0004602 IDA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    lipid metabolic process GO:0006629 IDA
    prostanoid metabolic process GO:0006692 IDA
    arachidonate metabolic process GO:0019369 IEA
    leukotriene biosynthetic process GO:0019370 IDA
    cellular oxidant detoxification GO:0098869 IEA
Subcellular Localization
    nuclear envelope GO:0005635 IBA
    mitochondrion GO:0005739 HTP
    mitochondrial outer membrane GO:0005741 IDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified MGST3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MGST3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Two-hybrid Homo sapiens
2 PDHA1 5160
Co-fractionation Homo sapiens
3 RAMP3  
Affinity Capture-MS Homo sapiens
4 DNAJC11 55735
Co-fractionation Homo sapiens
5 TOR1A 1861
Affinity Capture-MS Homo sapiens
6 KLK8 11202
Affinity Capture-MS Homo sapiens
7 TSPAN15 23555
Affinity Capture-MS Homo sapiens
8 CMTM5  
Two-hybrid Homo sapiens
9 VAPA 9218
Affinity Capture-MS Homo sapiens
10 GIMAP5  
Two-hybrid Homo sapiens
11 SLC39A4 55630
Affinity Capture-MS Homo sapiens
12 ACOT9 23597
Co-fractionation Homo sapiens
13 SGCA  
Affinity Capture-MS Homo sapiens
14 ATP6V1C1 528
Co-fractionation Homo sapiens
15 TMEM184A  
Affinity Capture-MS Homo sapiens
16 GAPDHS 26330
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SLC2A1 6513
Affinity Capture-MS Homo sapiens
18 HRAS 3265
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 KIF20A 10112
Affinity Capture-MS Homo sapiens
20 SLC25A1 6576
Co-fractionation Homo sapiens
21 PLN  
Two-hybrid Homo sapiens
22 TMEM72  
Affinity Capture-MS Homo sapiens
23 MRPL49 740
Co-fractionation Homo sapiens
24 S100A13 6284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 APOD 347
Two-hybrid Homo sapiens
26 Tubb4b 227613
Affinity Capture-MS Mus musculus
27 LRPPRC 10128
Co-fractionation Homo sapiens
28 GIMAP1  
Two-hybrid Homo sapiens
29 ARL6IP6  
Two-hybrid Homo sapiens
30 GP5 2814
Affinity Capture-MS Homo sapiens
31 NDUFS3 4722
Co-fractionation Homo sapiens
32 TFAM 7019
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
33 MCM2 4171
Affinity Capture-MS Homo sapiens
34 COX7A2L 9167
Co-fractionation Homo sapiens
35 NCR3LG1 374383
Affinity Capture-MS Homo sapiens
36 GRM2 2912
Two-hybrid Homo sapiens
37 SLC35A1 10559
Two-hybrid Homo sapiens
38 C2orf82  
Two-hybrid Homo sapiens
39 STX8 9482
Two-hybrid Homo sapiens
40 COA3 28958
Co-fractionation Homo sapiens
41 C14orf180  
Two-hybrid Homo sapiens
42 MRPS26 64949
Co-fractionation Homo sapiens
43 ATP2A3 489
Affinity Capture-MS Homo sapiens
44 LPAR3  
Two-hybrid Homo sapiens
45 C3orf52  
Two-hybrid Homo sapiens
46 NEU1 4758
Two-hybrid Homo sapiens
47 SVIP 258010
Affinity Capture-MS Homo sapiens
48 TMEM140  
Two-hybrid Homo sapiens
49 NDUFV1 4723
Co-fractionation Homo sapiens
50 TOMM40 10452
Co-fractionation Homo sapiens
51 BET1 10282
Two-hybrid Homo sapiens
52 TMEM107 84314
Two-hybrid Homo sapiens
53 ANKRD34C  
Co-fractionation Homo sapiens
54 NMT1 4836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 COX2 4513
Co-fractionation Homo sapiens
56 C14orf1 11161
Two-hybrid Homo sapiens
57 UQCRQ 27089
Co-fractionation Homo sapiens
58 ITGAM 3684
Two-hybrid Homo sapiens
59 KIF14 9928
Affinity Capture-MS Homo sapiens
60 IMPDH1 3614
Affinity Capture-MS Homo sapiens
61 UNK  
Affinity Capture-RNA Homo sapiens
62 EFNA2  
Affinity Capture-MS Homo sapiens
63 AQP1 358
Two-hybrid Homo sapiens
64 NDUFA9 4704
Co-fractionation Homo sapiens
65 NDUFS4 4724
Co-fractionation Homo sapiens
66 TSPAN33 340348
Two-hybrid Homo sapiens
67 NSG1  
Two-hybrid Homo sapiens
68 FUNDC2 65991
Two-hybrid Homo sapiens
69 HSP90B1 7184
Co-fractionation Homo sapiens
70 TSPAN12  
Two-hybrid Homo sapiens
71 TSPAN2 10100
Two-hybrid Homo sapiens
72 SNAP47 116841
Two-hybrid Homo sapiens
73 AGTRAP 57085
Two-hybrid Homo sapiens
74 LRP10 26020
Two-hybrid Homo sapiens
75 SLC25A20 788
Co-fractionation Homo sapiens
76 USMG5 84833
Co-fractionation Homo sapiens
77 APOL2 23780
Two-hybrid Homo sapiens
78 YIF1A 10897
Two-hybrid Homo sapiens
79 VCP 7415
Co-fractionation Homo sapiens
80 C20orf24  
Co-fractionation Homo sapiens
81 SLC2A5 6518
Two-hybrid Homo sapiens
82 SSUH2  
Affinity Capture-MS Homo sapiens
83 OCIAD2 132299
Co-fractionation Homo sapiens
84 NMT2 9397
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 TMEM205 374882
Co-fractionation Homo sapiens
86 SCN3B  
Two-hybrid Homo sapiens
87 C17orf62 79415
Two-hybrid Homo sapiens
88 VDAC2 7417
Co-fractionation Homo sapiens
89 NIPSNAP1 8508
Co-fractionation Homo sapiens
90 SLC7A1 6541
Two-hybrid Homo sapiens
91 COX4I1 1327
Co-fractionation Homo sapiens
92 CDIPT 10423
Two-hybrid Homo sapiens
93 TMEM60  
Two-hybrid Homo sapiens
94 LEMD1  
Two-hybrid Homo sapiens
95 NINJ2  
Two-hybrid Homo sapiens
96 MRM1  
Two-hybrid Homo sapiens
97 Lin7c 22343
Affinity Capture-MS Mus musculus
98 APLNR  
Affinity Capture-MS Homo sapiens
99 IRF9 10379
Affinity Capture-MS Homo sapiens
100 HADHB 3032
Co-fractionation Homo sapiens
101 HADHA 3030
Co-fractionation Homo sapiens
102 CANX 821
Co-fractionation Homo sapiens
103 CCNC  
Two-hybrid Homo sapiens
104 ZFPL1 7542
Two-hybrid Homo sapiens
105 GLMN 11146
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 MTIF3  
Two-hybrid Homo sapiens
107 UQCRC2 7385
Co-fractionation Homo sapiens
108 HMOX2 3163
Two-hybrid Homo sapiens
109 CLRN1  
Two-hybrid Homo sapiens
110 GOLT1B 51026
Affinity Capture-MS Homo sapiens
111 Rmdn3  
Affinity Capture-MS Mus musculus
112 CYB5R3 1727
Co-fractionation Homo sapiens
113 FXYD6  
Two-hybrid Homo sapiens
114 RAP1A 5906
Affinity Capture-MS Homo sapiens
115 NRM 11270
Two-hybrid Homo sapiens
116 FAXDC2  
Two-hybrid Homo sapiens
117 AQP3  
Two-hybrid Homo sapiens
118 CREB3L1  
Two-hybrid Homo sapiens
119 GPR152  
Two-hybrid Homo sapiens
120 TMEM86B  
Two-hybrid Homo sapiens
121 GPX8 493869
Two-hybrid Homo sapiens
122 BIRC6 57448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 ATP5J2 9551
Co-fractionation Homo sapiens
124 ZMPSTE24 10269
Co-fractionation Homo sapiens
125 TMEM229B  
Two-hybrid Homo sapiens
126 PPP2R1A 5518
Co-fractionation Homo sapiens
127 RPA3 6119
Proximity Label-MS Homo sapiens
128 COX6C 1345
Co-fractionation Homo sapiens
129 CYC1 1537
Co-fractionation Homo sapiens
130 BMP10  
Two-hybrid Homo sapiens
131 ARL6IP1 23204
Two-hybrid Homo sapiens
132 UQCRC1 7384
Co-fractionation Homo sapiens
133 AQP10  
Two-hybrid Homo sapiens
134 DDRGK1 65992
Affinity Capture-MS Homo sapiens
135 PLP2 5355
Two-hybrid Homo sapiens
136 SYS1  
Two-hybrid Homo sapiens
137 CACNG1  
Two-hybrid Homo sapiens
138 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
139 MIP  
Two-hybrid Homo sapiens
140 NKG7  
Two-hybrid Homo sapiens
141 FFAR1  
Affinity Capture-MS Homo sapiens
142 PPAPDC1A  
Two-hybrid Homo sapiens
143 SCCPDH 51097
Co-fractionation Homo sapiens
144 TMX2 51075
Two-hybrid Homo sapiens
145 GBAS 2631
Co-fractionation Homo sapiens
146 NRG1 3084
Affinity Capture-MS Homo sapiens
147 UNC50  
Two-hybrid Homo sapiens
148 SLC25A11 8402
Co-fractionation Homo sapiens
149 NAAA  
Affinity Capture-MS Homo sapiens
150 EDNRA 1909
Affinity Capture-MS Homo sapiens
151 PLP1 5354
Two-hybrid Homo sapiens
152 TOMM22 56993
Co-fractionation Homo sapiens
153 SMCO4  
Two-hybrid Homo sapiens
154 VAC14 55697
Affinity Capture-MS Homo sapiens
155 PDHB 5162
Co-fractionation Homo sapiens
156 HAVCR2  
Affinity Capture-MS Homo sapiens
157 BCKDHA 593
Co-fractionation Homo sapiens
158 FAM3C 10447
Co-fractionation Homo sapiens
159 COX5A 9377
Co-fractionation Homo sapiens
160 VAMP4 8674
Two-hybrid Homo sapiens
161 TSPAN17 26262
Affinity Capture-MS Homo sapiens
162 NDUFS7 374291
Co-fractionation Homo sapiens
163 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
164 COX8A  
Co-fractionation Homo sapiens
165 WIF1 11197
Affinity Capture-MS Homo sapiens
166 UFL1 23376
Affinity Capture-MS Homo sapiens
167 NME2P1 283458
Affinity Capture-MS Homo sapiens
168 CGRRF1  
Affinity Capture-MS Homo sapiens
169 STX3 6809
Two-hybrid Homo sapiens
170 SPPL2B 56928
Affinity Capture-MS Homo sapiens
171 MGST3 4259
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
172 TMEM109 79073
Two-hybrid Homo sapiens
173 LPAR2 9170
Affinity Capture-MS Homo sapiens
174 ATP5C1 509
Co-fractionation Homo sapiens
175 COX7C 1350
Co-fractionation Homo sapiens
176 VDAC3 7419
Co-fractionation Homo sapiens
177 HSPA12A 259217
Affinity Capture-MS Homo sapiens
178 C11orf24  
Two-hybrid Homo sapiens
179 UBIAD1 29914
Two-hybrid Homo sapiens
180 TOMM5  
Co-fractionation Homo sapiens
181 TMEM120B  
Two-hybrid Homo sapiens
View the network image/svg+xml



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