Gene description for KLK8
Gene name kallikrein-related peptidase 8
Gene symbol KLK8
Other names/aliases HNP
NP
NRPN
PRSS19
TADG14
Species Homo sapiens
 Database cross references - KLK8
ExoCarta ExoCarta_11202
Vesiclepedia VP_11202
Entrez Gene 11202
HGNC 6369
MIM 605644
UniProt O60259  
 KLK8 identified in sEVs derived from the following tissue/cell type
Keratinocytes 19530224    
Keratinocytes 19530224    
 Gene ontology annotations for KLK8
Molecular Function
    serine-type endopeptidase activity GO:0004252 IBA
    serine-type endopeptidase activity GO:0004252 IDA
    protein binding GO:0005515 IPI
Biological Process
    proteolysis GO:0006508 IEA
    memory GO:0007613 ISS
    response to wounding GO:0009611 ISS
    keratinocyte proliferation GO:0043616 ISS
    neuron projection morphogenesis GO:0048812 ISS
    regulation of synapse organization GO:0050807 ISS
    synapse organization GO:0050808 IEA
    protein maturation GO:0051604 IBA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISS
    cytoplasm GO:0005737 IDA
    secretory granule GO:0030141 IBA
    serine protease inhibitor complex GO:0097180 IDA
 Experiment description of studies that identified KLK8 in sEVs
1
Experiment ID 189
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 190
MISEV standards
EM
Biophysical techniques
HSC70
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KLK8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HEATR6  
Affinity Capture-MS Homo sapiens
2 RAB39A 54734
Affinity Capture-MS Homo sapiens
3 NOTCH2NL 388677
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 IL17RA 23765
Affinity Capture-MS Homo sapiens
5 RAB39B 116442
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TIMM17A  
Affinity Capture-MS Homo sapiens
7 DDX19B 11269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ZDHHC17 23390
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 ARF5 381
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 KRT34  
Two-hybrid Homo sapiens
11 NBPF19  
Two-hybrid Homo sapiens
12 RGS2  
Two-hybrid Homo sapiens
13 SLC25A15  
Affinity Capture-MS Homo sapiens
14 KRTAP1-1  
Two-hybrid Homo sapiens
15 KRTAP5-9  
Two-hybrid Homo sapiens
16 KRTAP10-3  
Two-hybrid Homo sapiens
17 SLC2A8 29988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ND1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SLC16A10 117247
Affinity Capture-MS Homo sapiens
20 VWDE  
Affinity Capture-MS Homo sapiens
21 TAMM41  
Affinity Capture-MS Homo sapiens
22 CYSRT1 375791
Two-hybrid Homo sapiens
23 KRTAP10-8  
Two-hybrid Homo sapiens
24 SLC1A4 6509
Affinity Capture-MS Homo sapiens
25 WFS1 7466
Affinity Capture-MS Homo sapiens
26 SLC25A4 291
Affinity Capture-MS Homo sapiens
27 ATP4A 495
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 LMF1 64788
Affinity Capture-MS Homo sapiens
29 MGST3 4259
Affinity Capture-MS Homo sapiens
30 SLC22A18 5002
Affinity Capture-MS Homo sapiens
31 HLA-C 3107
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which KLK8 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here