Gene description for ATP4A
Gene name ATPase, H+/K+ exchanging, alpha polypeptide
Gene symbol ATP4A
Other names/aliases ATP6A
Species Homo sapiens
 Database cross references - ATP4A
ExoCarta ExoCarta_495
Vesiclepedia VP_495
Entrez Gene 495
HGNC 819
MIM 137216
UniProt P20648  
 ATP4A identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Colorectal cancer cells 19837982    
Colorectal cancer cells 34887515    
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for ATP4A
Molecular Function
    magnesium ion binding GO:0000287 ISS
    P-type sodium:potassium-exchanging transporter activity GO:0005391 IBA
    ATP binding GO:0005524 IEA
    P-type potassium:proton transporter activity GO:0008900 IBA
    P-type potassium:proton transporter activity GO:0008900 TAS
    ATP hydrolysis activity GO:0016887 IEA
    potassium ion binding GO:0030955 ISS
Biological Process
    intracellular sodium ion homeostasis GO:0006883 IBA
    response to xenobiotic stimulus GO:0009410 IEA
    regulation of proton transport GO:0010155 IEA
    establishment or maintenance of transmembrane electrochemical gradient GO:0010248 IEA
    intracellular potassium ion homeostasis GO:0030007 IBA
    monoatomic ion transmembrane transport GO:0034220 TAS
    sodium ion export across plasma membrane GO:0036376 IBA
    pH reduction GO:0045851 IEA
    potassium ion transmembrane transport GO:0071805 ISO
    proton transmembrane transport GO:1902600 IBA
    potassium ion import across plasma membrane GO:1990573 IBA
Subcellular Localization
    extracellular space GO:0005615 HDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    potassium:proton exchanging ATPase complex GO:0005889 ISO
    apical plasma membrane GO:0016324 IEA
 Experiment description of studies that identified ATP4A in sEVs
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
4
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ATP4A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
2 HSD3B2 3284
Affinity Capture-MS Homo sapiens
3 TPRA1  
Affinity Capture-MS Homo sapiens
4 GPM6A 2823
Affinity Capture-MS Homo sapiens
5 LGR4 55366
Affinity Capture-MS Homo sapiens
6 KLK8 11202
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 RAB1A 5861
Affinity Capture-MS Homo sapiens
8 NAPA 8775
Affinity Capture-MS Homo sapiens
9 CD163 9332
Affinity Capture-MS Homo sapiens
10 ARL6IP5 10550
Affinity Capture-MS Homo sapiens
11 PSCA 8000
Affinity Capture-MS Homo sapiens
12 GNAQ 2776
Affinity Capture-MS Homo sapiens
13 VAMP2 6844
Affinity Capture-MS Homo sapiens
14 FAM3B 54097
Affinity Capture-MS Homo sapiens
15 ANXA6 309
Affinity Capture-MS Homo sapiens
16 PTPN1 5770
Affinity Capture-MS Homo sapiens
17 SLC12A2 6558
Affinity Capture-MS Homo sapiens
18 ERO1LB 56605
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
20 LSS 4047
Affinity Capture-MS Homo sapiens
21 SLC26A9 115019
Affinity Capture-MS Homo sapiens
22 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 CPD 1362
Affinity Capture-MS Homo sapiens
24 STX7 8417
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
25 VAT1 10493
Affinity Capture-MS Homo sapiens
26 CDCA8 55143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 LMAN2 10960
Affinity Capture-MS Homo sapiens
28 APOH 350
Affinity Capture-MS Homo sapiens
29 LIPC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 FAF2 23197
Affinity Capture-MS Homo sapiens
31 RAB25 57111
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
32 RRP7A 27341
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 ATP1A3 478
Affinity Capture-MS Homo sapiens
34 BSG 682
Affinity Capture-MS Homo sapiens
35 ANXA7 310
Affinity Capture-MS Homo sapiens
36 SYPL1 6856
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
37 GNAI1 2770
Affinity Capture-MS Homo sapiens
38 UBXN8  
Affinity Capture-MS Homo sapiens
39 SCAMP2 10066
Affinity Capture-MS Homo sapiens
40 TFR2 7036
Affinity Capture-MS Homo sapiens
41 ATP4B  
Affinity Capture-MS Homo sapiens
42 ARMC1 55156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 VAMP1 6843
Affinity Capture-MS Homo sapiens
44 GANAB 23193
Affinity Capture-MS Homo sapiens
45 ATP4A 495
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
46 GNAI2 2771
Affinity Capture-MS Homo sapiens
47 RAB10 10890
Affinity Capture-MS Homo sapiens
48 KCNE2  
Affinity Capture-Western Homo sapiens
49 LIPF  
Affinity Capture-MS Homo sapiens
50 CA9 768
Affinity Capture-MS Homo sapiens
51 BCAP31 10134
Affinity Capture-MS Homo sapiens
52 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 VAMP8 8673
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
54 AHNAK 79026
Affinity Capture-MS Homo sapiens
55 ANXA1 301
Affinity Capture-MS Homo sapiens
56 AOC3 8639
Affinity Capture-MS Homo sapiens
57 VAMP3 9341
Affinity Capture-MS Homo sapiens
58 SKP2  
Affinity Capture-MS Homo sapiens
59 AGR2 10551
Affinity Capture-MS Homo sapiens
60 GIF  
Affinity Capture-MS Homo sapiens
61 ATP6V1A 523
Affinity Capture-MS Homo sapiens
62 RAB11B 9230
Affinity Capture-MS Homo sapiens
63 ATP1B1 481
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 PGC  
Affinity Capture-MS Homo sapiens
65 CLTC 1213
Affinity Capture-MS Homo sapiens
66 HAX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 ACSL1 2180
Affinity Capture-MS Homo sapiens
68 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
69 MRC1  
Affinity Capture-MS Homo sapiens
70 GNG2 54331
Affinity Capture-MS Homo sapiens
71 SYNGR2 9144
Affinity Capture-MS Homo sapiens
72 M6PR 4074
Affinity Capture-MS Homo sapiens
73 MAL2 114569
Affinity Capture-MS Homo sapiens
74 SCAMP3 10067
Affinity Capture-MS Homo sapiens
75 EPHX1 2052
Affinity Capture-MS Homo sapiens
76 KCNQ1  
Affinity Capture-MS Homo sapiens
77 SCARB2 950
Affinity Capture-MS Homo sapiens
78 UFL1 23376
Two-hybrid Homo sapiens
79 ANXA11 311
Affinity Capture-MS Homo sapiens
80 STX3 6809
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
81 AIRE  
Affinity Capture-MS Homo sapiens
82 RAB7A 7879
Proximity Label-MS Homo sapiens
83 ATP1A1 476
Affinity Capture-MS Homo sapiens
84 RAB11A 8766
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
85 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 VAMP5 10791
Affinity Capture-MS Homo sapiens
87 RAB5C 5878
Affinity Capture-MS Homo sapiens
88 ANXA2 302
Affinity Capture-MS Homo sapiens
89 APOA1 335
Affinity Capture-MS Homo sapiens
90 CAV1 857
Affinity Capture-MS Homo sapiens
91 CTNND1 1500
Affinity Capture-MS Homo sapiens
92 FAM3C 10447
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ATP4A is involved
PathwayEvidenceSource
Ion channel transport TAS Reactome
Ion transport by P-type ATPases TAS Reactome
Transport of small molecules TAS Reactome





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