Gene description for VAMP1
Gene name vesicle-associated membrane protein 1 (synaptobrevin 1)
Gene symbol VAMP1
Other names/aliases SPAX1
SYB1
VAMP-1
Species Homo sapiens
 Database cross references - VAMP1
ExoCarta ExoCarta_6843
Vesiclepedia VP_6843
Entrez Gene 6843
HGNC 12642
MIM 185880
UniProt P23763  
 VAMP1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Neuroblastoma cells 25944692    
 Gene ontology annotations for VAMP1
Molecular Function
    protein binding GO:0005515 IPI
    SNARE binding GO:0000149 IBA
    SNAP receptor activity GO:0005484 IBA
Biological Process
    exocytosis GO:0006887 IBA
    synaptic transmission GO:0007268 IEA
    vesicle fusion GO:0006906 IBA
    SNARE complex assembly GO:0035493 IEA
Subcellular Localization
    cell surface GO:0009986 IEA
    specific granule membrane GO:0035579 IDA
    SNARE complex GO:0031201 IBA
    cytosol GO:0005829 TAS
    neuron projection GO:0043005 IEA
    cell junction GO:0030054 IEA
    synaptic vesicle GO:0008021 IBA
    integral component of plasma membrane GO:0005887 IEA
    tertiary granule membrane GO:0070821 IDA
    azurophil granule membrane GO:0035577 IDA
    mitochondrial outer membrane GO:0005741 IEA
    synaptic vesicle membrane GO:0030672 TAS
 Experiment description of studies that identified VAMP1 in exosomes
1
Experiment ID 21
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for VAMP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STX1B 112755
Two-hybrid Homo sapiens
2 STX1A 6804
Two-hybrid Homo sapiens
3 VAPB 9217
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
4 SNAP23 8773
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
5 VAPA 9218
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
6 STX4 6810
Two-hybrid Homo sapiens
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