Gene description for VAMP1
Gene name vesicle-associated membrane protein 1 (synaptobrevin 1)
Gene symbol VAMP1
Other names/aliases SPAX1
SYB1
VAMP-1
Species Homo sapiens
 Database cross references - VAMP1
ExoCarta ExoCarta_6843
Vesiclepedia VP_6843
Entrez Gene 6843
HGNC 12642
MIM 185880
UniProt P23763  
 VAMP1 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Neuroblastoma cells 25944692    
 Gene ontology annotations for VAMP1
Molecular Function
    SNAP receptor activity GO:0005484 IBA
    protein binding GO:0005515 IPI
    syntaxin binding GO:0019905 IBA
Biological Process
    vesicle fusion GO:0006906 IBA
    SNARE complex assembly GO:0035493 IBA
Subcellular Localization
    mitochondrial outer membrane GO:0005741 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    synaptic vesicle membrane GO:0030672 TAS
    SNARE complex GO:0031201 IBA
    azurophil granule membrane GO:0035577 IDA
    specific granule membrane GO:0035579 IDA
    neuron projection GO:0043005 IEA
    tertiary granule membrane GO:0070821 IDA
 Experiment description of studies that identified VAMP1 in sEVs
1
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for VAMP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TMEM35  
Two-hybrid Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 SLC30A8  
Two-hybrid Homo sapiens
4 RNF24  
Two-hybrid Homo sapiens
5 VAPB 9217
Reconstituted Complex Homo sapiens
6 VAPA 9218
Reconstituted Complex Homo sapiens
7 CRB3 92359
Two-hybrid Homo sapiens
8 FCER1G 2207
Two-hybrid Homo sapiens
9 ERGIC3 51614
Two-hybrid Homo sapiens
10 TMEM14B  
Two-hybrid Homo sapiens
11 VAMP3 9341
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
12 VAMP2 6844
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
13 REEP4  
Two-hybrid Homo sapiens
14 CCDC155  
Two-hybrid Homo sapiens
15 STX4 6810
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 CREB3L1  
Two-hybrid Homo sapiens
17 APEX1 328
Affinity Capture-RNA Homo sapiens
18 GPR152  
Two-hybrid Homo sapiens
19 LAPTM5 7805
Two-hybrid Homo sapiens
20 STX1B 112755
Two-hybrid Homo sapiens
21 EBP  
Two-hybrid Homo sapiens
22 FAM134C 162427
Two-hybrid Homo sapiens
23 STX1A 6804
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
24 SGMS2 166929
Two-hybrid Homo sapiens
25 ELOVL7 79993
Two-hybrid Homo sapiens
26 SEC11C 90701
Two-hybrid Homo sapiens
27 TMX2 51075
Two-hybrid Homo sapiens
28 ATP4A 495
Affinity Capture-MS Homo sapiens
29 ARL13B 200894
Two-hybrid Homo sapiens
30 SCN3B  
Two-hybrid Homo sapiens
31 ELOVL4  
Two-hybrid Homo sapiens
32 FAM210B  
Two-hybrid Homo sapiens
33 SNAP29 9342
Two-hybrid Homo sapiens
View the network image/svg+xml



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