Gene description for CRB3
Gene name crumbs family member 3
Gene symbol CRB3
Other names/aliases -
Species Homo sapiens
 Database cross references - CRB3
ExoCarta ExoCarta_92359
Vesiclepedia VP_92359
Entrez Gene 92359
HGNC 20237
MIM 609737
UniProt Q9BUF7  
 CRB3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Urine 19056867    
 Gene ontology annotations for CRB3
Molecular Function
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IPI
    protein domain specific binding GO:0019904 IPI
Biological Process
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 NAS
    protein localization to plasma membrane GO:0072659 IDA
    positive regulation of cell junction assembly GO:1901890 ISS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    bicellular tight junction GO:0005923 IEA
    apical plasma membrane GO:0016324 NAS
    cell junction GO:0030054 IDA
    subapical complex GO:0035003 IEA
    apical junction complex GO:0043296 NAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CRB3 in exosomes
1
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CRB3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BET1 10282
Two-hybrid Homo sapiens
2 PSMA3 5684
Two-hybrid Homo sapiens
3 VTI1B 10490
Two-hybrid Homo sapiens
4 YIPF6 286451
Two-hybrid Homo sapiens
5 PSENEN  
Two-hybrid Homo sapiens
6 HMOX2 3163
Two-hybrid Homo sapiens
7 TREX1  
Two-hybrid Homo sapiens
8 MIB1 57534
Co-localization Homo sapiens
9 FUNDC2 65991
Two-hybrid Homo sapiens
10 PLLP  
Two-hybrid Homo sapiens
11 RABAC1 10567
Two-hybrid Homo sapiens
12 PARD6B 84612
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 MPP5 64398
Affinity Capture-MS Homo sapiens
14 TMEM222 84065
Two-hybrid Homo sapiens
15 WDR83  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 SERF1B  
Two-hybrid Homo sapiens
17 VAMP1 6843
Two-hybrid Homo sapiens
18 PLP2 5355
Two-hybrid Homo sapiens
19 NDUFA3  
Two-hybrid Homo sapiens
20 STX5 6811
Two-hybrid Homo sapiens
21 ANKRD46 157567
Two-hybrid Homo sapiens
22 SERP1 27230
Two-hybrid Homo sapiens
23 UBIAD1 29914
Two-hybrid Homo sapiens
24 SERF1A  
Two-hybrid Homo sapiens
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