Gene description for ANKRD46
Gene name ankyrin repeat domain 46
Gene symbol ANKRD46
Other names/aliases ANK-S
GENX-115279
Species Homo sapiens
 Database cross references - ANKRD46
ExoCarta ExoCarta_157567
Vesiclepedia VP_157567
Entrez Gene 157567
HGNC 27229
UniProt Q86W74  
 ANKRD46 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ANKRD46
Molecular Function
    protein binding GO:0005515 IPI
Subcellular Localization
    membrane GO:0016020 IEA
 Experiment description of studies that identified ANKRD46 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ANKRD46
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HMP19  
Two-hybrid Homo sapiens
2 RNF149 284996
Affinity Capture-MS Homo sapiens
3 HSD17B11 51170
Two-hybrid Homo sapiens
4 SLC10A6  
Two-hybrid Homo sapiens
5 SEP15 9403
Affinity Capture-MS Homo sapiens
6 SRPRB 58477
Affinity Capture-MS Homo sapiens
7 BSCL2  
Two-hybrid Homo sapiens
8 BNIP1 662
Affinity Capture-MS Homo sapiens
9 FAM3B 54097
Affinity Capture-MS Homo sapiens
10 ZMPSTE24 10269
Affinity Capture-MS Homo sapiens
11 STX4 6810
Two-hybrid Homo sapiens
12 TMX1 81542
Affinity Capture-MS Homo sapiens
13 AMIGO1  
Two-hybrid Homo sapiens
14 APEX1 328
Affinity Capture-RNA Homo sapiens
15 ADAM21  
Affinity Capture-MS Homo sapiens
16 TMPRSS2 7113
Two-hybrid Homo sapiens
17 STX1A 6804
Two-hybrid Homo sapiens
18 ATRNL1 26033
Affinity Capture-MS Homo sapiens
19 TIMMDC1  
Two-hybrid Homo sapiens
20 FAM174A 345757
Two-hybrid Homo sapiens
21 PVRIG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MUC1 4582
Two-hybrid Homo sapiens
23 ST14 6768
Affinity Capture-MS Homo sapiens
24 PGRMC2 10424
Affinity Capture-MS Homo sapiens
25 PSG8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
27 TMEM259 91304
Affinity Capture-MS Homo sapiens
28 KIR2DL3  
Two-hybrid Homo sapiens
29 TSHB  
Affinity Capture-MS Homo sapiens
30 TMEM106A  
Affinity Capture-MS Homo sapiens
31 FAF2 23197
Affinity Capture-MS Homo sapiens
32 HLA-G 3135
Affinity Capture-MS Homo sapiens
33 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 TMEM248 55069
Two-hybrid Homo sapiens
35 VEZT 55591
Affinity Capture-MS Homo sapiens
36 CLDN7 1366
Two-hybrid Homo sapiens
37 SHISA3  
Two-hybrid Homo sapiens
38 ST3GAL1 6482
Affinity Capture-MS Homo sapiens
39 ST6GALNAC6 30815
Two-hybrid Homo sapiens
40 CXADR 1525
Two-hybrid Homo sapiens
41 RELL2  
Two-hybrid Homo sapiens
42 CD1E  
Affinity Capture-MS Homo sapiens
43 ITM2C 81618
Affinity Capture-MS Homo sapiens
44 PON2 5445
Affinity Capture-MS Homo sapiens
45 TMEM45B 120224
Two-hybrid Homo sapiens
46 GML  
Affinity Capture-MS Homo sapiens
47 SPACA3  
Affinity Capture-MS Homo sapiens
48 ARL13B 200894
Two-hybrid Homo sapiens
49 SCN3B  
Two-hybrid Homo sapiens
50 STX2 2054
Two-hybrid Homo sapiens
51 MFF 56947
Two-hybrid Homo sapiens
52 CST9  
Affinity Capture-MS Homo sapiens
53 MEGF8 1954
Affinity Capture-MS Homo sapiens
54 NBAS 51594
Affinity Capture-MS Homo sapiens
55 CANX 821
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 LRRC25  
Two-hybrid Homo sapiens
57 SECTM1 6398
Two-hybrid Homo sapiens
58 CRB3 92359
Two-hybrid Homo sapiens
59 ATRN 8455
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 CBLN4  
Affinity Capture-MS Homo sapiens
61 TSPAN18 90139
Affinity Capture-MS Homo sapiens
62 CCDC155  
Two-hybrid Homo sapiens
63 HSD17B13  
Two-hybrid Homo sapiens
64 IL5RA  
Affinity Capture-MS Homo sapiens
65 INSL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 SUSD3 203328
Two-hybrid Homo sapiens
67 MGRN1 23295
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 GPR152  
Two-hybrid Homo sapiens
69 FAM209A 200232
Two-hybrid Homo sapiens
70 BTNL2  
Affinity Capture-MS Homo sapiens
71 GPR42  
Two-hybrid Homo sapiens
72 BMP5 653
Affinity Capture-MS Homo sapiens
73 FAM134C 162427
Two-hybrid Homo sapiens
74 CLEC4D  
Two-hybrid Homo sapiens
75 FBXO2 26232
Affinity Capture-MS Homo sapiens
76 PSCA 8000
Affinity Capture-MS Homo sapiens
77 PPAPDC1A  
Two-hybrid Homo sapiens
78 AP1M2 10053
Affinity Capture-MS Homo sapiens
79 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
80 EFNB2 1948
Two-hybrid Homo sapiens
81 CLDN20  
Two-hybrid Homo sapiens
82 CREB3L1  
Two-hybrid Homo sapiens
83 TMEM31  
Two-hybrid Homo sapiens
84 CD46 4179
Two-hybrid Homo sapiens
85 INHBE 83729
Affinity Capture-MS Homo sapiens
86 SPCS3 60559
Affinity Capture-MS Homo sapiens
87 PSG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 PSG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 NDP  
Affinity Capture-MS Homo sapiens
90 SLC16A2  
Two-hybrid Homo sapiens
91 LDLRAD1  
Two-hybrid Homo sapiens
92 ADAM33 80332
Affinity Capture-MS Homo sapiens
93 LMAN2L 81562
Affinity Capture-MS Homo sapiens
94 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
95 EBP  
Two-hybrid Homo sapiens
96 SLAMF1 6504
Affinity Capture-MS Homo sapiens
97 IFNLR1  
Two-hybrid Homo sapiens
98 MMGT1 93380
Two-hybrid Homo sapiens
99 SIRPD  
Affinity Capture-MS Homo sapiens
100 THAP4 51078
Two-hybrid Homo sapiens
101 SEC62 7095
Affinity Capture-MS Homo sapiens
102 TMEM79  
Two-hybrid Homo sapiens
103 C16orf54 283897
Two-hybrid Homo sapiens
104 RAB7A 7879
Proximity Label-MS Homo sapiens
105 ALPP 250
Affinity Capture-MS Homo sapiens
106 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 EDN3  
Affinity Capture-MS Homo sapiens
108 APH1A 51107
Two-hybrid Homo sapiens
109 GOLM1 51280
Two-hybrid Homo sapiens
110 GPHA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 RNF157 114804
Affinity Capture-MS Homo sapiens
112 PTCH1  
Affinity Capture-MS Homo sapiens
113 ELOVL4  
Two-hybrid Homo sapiens
114 HLA-C 3107
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ANKRD46 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here