Gene description for TMPRSS2
Gene name transmembrane protease, serine 2
Gene symbol TMPRSS2
Other names/aliases PP9284
PRSS10
Species Homo sapiens
 Database cross references - TMPRSS2
ExoCarta ExoCarta_7113
Vesiclepedia VP_7113
Entrez Gene 7113
HGNC 11876
MIM 602060
UniProt O15393  
 TMPRSS2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Saliva 19199708    
Urine 15326289    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for TMPRSS2
Molecular Function
    serine-type endopeptidase activity GO:0004252 IDA
    serine-type endopeptidase activity GO:0004252 TAS
    protein binding GO:0005515 IPI
    serine-type peptidase activity GO:0008236 TAS
Biological Process
    proteolysis GO:0006508 IDA
    protein autoprocessing GO:0016540 IBA
    protein autoprocessing GO:0016540 IMP
    viral translation GO:0019081 TAS
    positive regulation of viral entry into host cell GO:0046598 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    nucleoplasm GO:0005654 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified TMPRSS2 in exosomes
1
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
6
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
7
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
8
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TMPRSS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UPK2 7379
Two-hybrid Homo sapiens
2 TMEM60  
Two-hybrid Homo sapiens
3 NINJ2  
Two-hybrid Homo sapiens
4 CMTM7  
Two-hybrid Homo sapiens
5 C2CD2L 9854
Two-hybrid Homo sapiens
6 MOSPD3  
Two-hybrid Homo sapiens
7 BMP10  
Two-hybrid Homo sapiens
8 PLP1 5354
Two-hybrid Homo sapiens
9 TMEM218  
Two-hybrid Homo sapiens
10 C14orf1 11161
Two-hybrid Homo sapiens
11 ZFPL1 7542
Two-hybrid Homo sapiens
12 TNF  
Two-hybrid Homo sapiens
13 SMIM1 388588
Two-hybrid Homo sapiens
14 AQP1 358
Two-hybrid Homo sapiens
15 TMEM120B  
Two-hybrid Homo sapiens
16 TMEM128  
Two-hybrid Homo sapiens
17 TMEM229B  
Two-hybrid Homo sapiens
18 DEFB103B  
Two-hybrid Homo sapiens
19 CLEC7A  
Two-hybrid Homo sapiens
20 IGFBP5 3488
Two-hybrid Homo sapiens
21 FAXDC2  
Two-hybrid Homo sapiens
22 TMEM11 8834
Two-hybrid Homo sapiens
23 PLLP  
Two-hybrid Homo sapiens
24 SFTPC  
Two-hybrid Homo sapiens
25 DEFB103A  
Two-hybrid Homo sapiens
26 TMEM86B  
Two-hybrid Homo sapiens
27 CNIH2  
Two-hybrid Homo sapiens
28 PLN  
Two-hybrid Homo sapiens
29 ADIPOQ 9370
Two-hybrid Homo sapiens
30 BRICD5  
Two-hybrid Homo sapiens
31 EDDM3B  
Two-hybrid Homo sapiens
32 CTXN3  
Two-hybrid Homo sapiens
33 TMEM79  
Two-hybrid Homo sapiens
34 TMEM243  
Two-hybrid Homo sapiens
35 TMEM222 84065
Two-hybrid Homo sapiens
36 SEC22A  
Two-hybrid Homo sapiens
37 BNIP2  
Two-hybrid Homo sapiens
38 TMEM86A  
Two-hybrid Homo sapiens
39 SLC35A1 10559
Two-hybrid Homo sapiens
40 VAMP5 10791
Two-hybrid Homo sapiens
41 STX8 9482
Two-hybrid Homo sapiens
42 PGAP2  
Two-hybrid Homo sapiens
43 CXCL9  
Two-hybrid Homo sapiens
44 CFHR5  
Two-hybrid Homo sapiens
45 PLP2 5355
Two-hybrid Homo sapiens
46 SELK  
Two-hybrid Homo sapiens
47 BNIP3 664
Two-hybrid Homo sapiens
48 ANKRD46 157567
Two-hybrid Homo sapiens
49 PTCH1  
Two-hybrid Homo sapiens
50 CNIH3  
Two-hybrid Homo sapiens
51 C17orf62 79415
Two-hybrid Homo sapiens
52 PTTG1IP 754
Two-hybrid Homo sapiens
53 C3orf52  
Two-hybrid Homo sapiens
54 C1QL4  
Two-hybrid Homo sapiens
55 FAM3C 10447
Two-hybrid Homo sapiens
56 CLN6  
Two-hybrid Homo sapiens
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