Gene description for SLC35A1
Gene name solute carrier family 35 (CMP-sialic acid transporter), member A1
Gene symbol SLC35A1
Other names/aliases CDG2F
CMPST
CST
hCST
Species Homo sapiens
 Database cross references - SLC35A1
ExoCarta ExoCarta_10559
Vesiclepedia VP_10559
Entrez Gene 10559
HGNC 11021
MIM 605634
UniProt P78382  
 SLC35A1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SLC35A1
Molecular Function
    CMP-N-acetylneuraminate transmembrane transporter activity GO:0005456 IBA
    CMP-N-acetylneuraminate transmembrane transporter activity GO:0005456 ISS
    CMP-N-acetylneuraminate transmembrane transporter activity GO:0005456 TAS
    protein binding GO:0005515 IPI
    antiporter activity GO:0015297 ISS
Biological Process
    carbohydrate metabolic process GO:0005975 TAS
    CMP-N-acetylneuraminate biosynthetic process GO:0006055 IEA
    CMP-N-acetylneuraminate transmembrane transport GO:0015782 IBA
    CMP-N-acetylneuraminate transmembrane transport GO:0015782 IDA
    CMP-N-acetylneuraminate transmembrane transport GO:0015782 ISS
    protein modification process GO:0036211 TAS
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 TAS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 ISS
 Experiment description of studies that identified SLC35A1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SLC35A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 F2RL1  
Two-hybrid Homo sapiens
2 F11R 50848
Two-hybrid Homo sapiens
3 GPX8 493869
Two-hybrid Homo sapiens
4 ICK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SDC3  
Two-hybrid Homo sapiens
6 FNDC9  
Two-hybrid Homo sapiens
7 EBAG9 9166
Two-hybrid Homo sapiens
8 SSMEM1  
Two-hybrid Homo sapiens
9 ASZ1  
Two-hybrid Homo sapiens
10 KIR3DL3  
Two-hybrid Homo sapiens
11 CD53 963
Two-hybrid Homo sapiens
12 ASGR2  
Two-hybrid Homo sapiens
13 ERGIC3 51614
Two-hybrid Homo sapiens
14 RELL2  
Two-hybrid Homo sapiens
15 SLC34A2 10568
Two-hybrid Homo sapiens
16 TMEM139  
Two-hybrid Homo sapiens
17 CPLX4  
Two-hybrid Homo sapiens
18 TCTA  
Two-hybrid Homo sapiens
19 MCFD2 90411
Two-hybrid Homo sapiens
20 FAM209A 200232
Two-hybrid Homo sapiens
21 FASN 2194
Negative Genetic Homo sapiens
22 C12orf49  
Positive Genetic Homo sapiens
23 TMPRSS2 7113
Two-hybrid Homo sapiens
24 MRPS18B 28973
Two-hybrid Homo sapiens
25 CASP3 836
Affinity Capture-MS Homo sapiens
26 PSCA 8000
Two-hybrid Homo sapiens
27 PPAPDC1A  
Two-hybrid Homo sapiens
28 FBXW7  
Synthetic Lethality Homo sapiens
29 ARL13B 200894
Two-hybrid Homo sapiens
30 SLC1A1 6505
Two-hybrid Homo sapiens
31 SYT2  
Two-hybrid Homo sapiens
32 LHFPL5  
Two-hybrid Homo sapiens
33 MGST3 4259
Two-hybrid Homo sapiens
34 TMX2 51075
Two-hybrid Homo sapiens
35 ACBD3 64746
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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