Gene description for SLC34A2
Gene name solute carrier family 34 (type II sodium/phosphate cotransporter), member 2
Gene symbol SLC34A2
Other names/aliases NAPI-3B
NAPI-IIb
NPTIIb
Species Homo sapiens
 Database cross references - SLC34A2
ExoCarta ExoCarta_10568
Entrez Gene 10568
HGNC 11020
MIM 604217
UniProt O95436  
 SLC34A2 identified in exosomes derived from the following tissue/cell type
Breast milk 17641064    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Tracheobronchial cells 19190083    
 Gene ontology annotations for SLC34A2
Molecular Function
    phosphate ion binding GO:0042301 IDA
    protein domain specific binding GO:0019904 IEA
    sodium:phosphate symporter activity GO:0005436 IDA
    sodium-dependent phosphate transmembrane transporter activity GO:0015321 IDA
    sodium ion binding GO:0031402 IDA
Biological Process
    ion transport GO:0006811 TAS
    sodium-dependent phosphate transport GO:0044341 IEA
    in utero embryonic development GO:0001701 IEA
    response to fructose GO:0009750 IEA
    transmembrane transport GO:0055085 TAS
    phosphate ion transmembrane transport GO:0035435 IDA
    cellular phosphate ion homeostasis GO:0030643 IDA
    phosphate ion transport GO:0006817 IDA
    aging GO:0007568 IEA
    sodium ion transmembrane transport GO:0035725 IDA
    response to estrogen GO:0043627 IEP
    response to estradiol GO:0032355 IEA
Subcellular Localization
    brush border membrane GO:0031526 ISS
    integral component of membrane GO:0016021 NAS
    plasma membrane GO:0005886 TAS
    microvillus membrane GO:0031528 IEA
    vesicle GO:0031982 IDA
    integral component of plasma membrane GO:0005887 NAS
    apical plasma membrane GO:0016324 IDA
 Experiment description of studies that identified SLC34A2 in exosomes
1
Experiment ID 48
ISEV standards
EM|IEM
EV Biophysical techniques
HSC70
EV Cytosolic markers
CD63|CD86|MHCII
EV Membrane markers
CANX
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 34
ISEV standards
EM|IEM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD63|MHCI|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19190083    
Organism Homo sapiens
Experiment description Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
Authors Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK
Journal name FASEB
Publication year 2009
Sample Tracheobronchial cells
Sample name Tracheobronchial epithelial cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.16 -1.18 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
FACS
 Protein-protein interactions for SLC34A2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SLC34A2 is involved
PathwayEvidenceSource
Type II Na+/Pi cotransporters TAS Reactome





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