Gene description for SLC34A2
Gene name solute carrier family 34 (type II sodium/phosphate cotransporter), member 2
Gene symbol SLC34A2
Other names/aliases NAPI-3B
NAPI-IIb
NPTIIb
Species Homo sapiens
 Database cross references - SLC34A2
ExoCarta ExoCarta_10568
Vesiclepedia VP_10568
Entrez Gene 10568
HGNC 11020
MIM 604217
UniProt O95436  
 SLC34A2 identified in sEVs derived from the following tissue/cell type
Breast milk 17641064    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Tracheobronchial cells 19190083    
 Gene ontology annotations for SLC34A2
Molecular Function
    sodium:phosphate symporter activity GO:0005436 IBA
    sodium:phosphate symporter activity GO:0005436 IDA
    sodium:phosphate symporter activity GO:0005436 TAS
    protein binding GO:0005515 IPI
    sodium ion binding GO:0031402 IDA
    phosphate ion binding GO:0042301 IDA
Biological Process
    in utero embryonic development GO:0001701 IEA
    phosphate ion transport GO:0006817 IDA
    intracellular phosphate ion homeostasis GO:0030643 IBA
    intracellular phosphate ion homeostasis GO:0030643 IDA
    sodium ion transmembrane transport GO:0035725 IEA
    response to estrogen GO:0043627 IEP
    sodium-dependent phosphate transport GO:0044341 IBA
Subcellular Localization
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    brush border GO:0005903 IBA
    membrane GO:0016020 IDA
    membrane GO:0016020 NAS
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISS
    brush border membrane GO:0031526 ISS
    vesicle GO:0031982 HDA
    vesicle GO:0031982 IBA
 Experiment description of studies that identified SLC34A2 in sEVs
1
Experiment ID 48
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|CD86|MHCII
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
2
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 34
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
TSG101|CD63|MHCI|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19190083    
Organism Homo sapiens
Experiment description Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
Authors "Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK"
Journal name FASEB
Publication year 2009
Sample Tracheobronchial cells
Sample name Tracheobronchial epithelial cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.16 -1.18 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
FACS
 Protein-protein interactions for SLC34A2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NUDCD1 84955
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
2 TMEM239  
Two-hybrid Homo sapiens
3 LOC100507537  
Two-hybrid Homo sapiens
4 GUCA2A  
Two-hybrid Homo sapiens
5 PLN  
Two-hybrid Homo sapiens
6 MYADM 91663
Affinity Capture-MS Homo sapiens
7 LINGO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SLC35A1 10559
Two-hybrid Homo sapiens
9 SYNGR2 9144
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CACNA2D1 781
Affinity Capture-MS Homo sapiens
11 ATP6V0D2 245972
Affinity Capture-MS Homo sapiens
12 LMTK2 22853
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 CYBRD1 79901
Affinity Capture-MS Homo sapiens
14 C14orf1 11161
Two-hybrid Homo sapiens
15 TMEM63B 55362
Affinity Capture-MS Homo sapiens
16 RTP2  
Two-hybrid Homo sapiens
17 KIAA1715 80856
Affinity Capture-MS Homo sapiens
18 TUSC5  
Two-hybrid Homo sapiens
19 ZDHHC3 51304
Affinity Capture-MS Homo sapiens
20 SLC39A7 7922
Two-hybrid Homo sapiens
21 CD47 961
Affinity Capture-MS Homo sapiens
22 TMEM128  
Two-hybrid Homo sapiens
23 ENDOD1 23052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PNLIPRP1  
Two-hybrid Homo sapiens
25 AIG1  
Two-hybrid Homo sapiens
26 TMEM42  
Two-hybrid Homo sapiens
27 FXYD6-FXYD2  
Two-hybrid Homo sapiens
28 RABL6 55684
Affinity Capture-MS Homo sapiens
29 JAGN1 84522
Two-hybrid Homo sapiens
30 ATP6V0C 527
Affinity Capture-MS Homo sapiens
31 TEX264 51368
Two-hybrid Homo sapiens
32 CMSS1  
Affinity Capture-MS Homo sapiens
33 PCDH10 57575
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 KRIT1 889
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 ZDHHC18 84243
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 SLC30A7 148867
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 C12orf43  
Affinity Capture-MS Homo sapiens
38 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 SLC30A3  
Two-hybrid Homo sapiens
40 QRSL1  
Affinity Capture-MS Homo sapiens
41 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 ATP11C 286410
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ATP6AP1 537
Affinity Capture-MS Homo sapiens
44 FLOT2 2319
Affinity Capture-MS Homo sapiens
45 LHFPL5  
Two-hybrid Homo sapiens
46 TSPO2  
Two-hybrid Homo sapiens
47 FLOT1 10211
Affinity Capture-MS Homo sapiens
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