Gene description for KRIT1
Gene name KRIT1, ankyrin repeat containing
Gene symbol KRIT1
Other names/aliases CAM
CCM1
Species Homo sapiens
 Database cross references - KRIT1
ExoCarta ExoCarta_889
Entrez Gene 889
HGNC 1573
MIM 604214
UniProt O00522  
 KRIT1 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Thymus 23844026    
 Gene ontology annotations for KRIT1
Molecular Function
    GTPase regulator activity GO:0030695 TAS
    microtubule binding GO:0008017 IDA
    protein binding GO:0005515 IPI
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    protein complex binding GO:0032403 IDA
Biological Process
    negative regulation of endothelial cell migration GO:0010596 IMP
    cell redox homeostasis GO:0045454 IMP
    regulation of establishment of cell polarity GO:2000114 IMP
    negative regulation of endothelial cell proliferation GO:0001937 IMP
    angiogenesis GO:0001525 IEA
    negative regulation of endothelial cell apoptotic process GO:2000352 IMP
    negative regulation of angiogenesis GO:0016525 IMP
    positive regulation of protein binding GO:0032092 IEA
    regulation of catalytic activity GO:0050790 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
Subcellular Localization
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 IDA
    cell-cell junction GO:0005911 IDA
    microtubule GO:0005874 IDA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified KRIT1 in exosomes
1
Experiment ID 260
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KRIT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RELA  
Invitro Homo sapiens
Invivo Homo sapiens
2 DAPK1  
Affinity Capture-Western Homo sapiens
3 ITGB1BP1 9270
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
4 RAP1A 5906
Two-hybrid Homo sapiens
5 EWSR1 2130
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which KRIT1 is involved
No pathways found





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